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1HIS肽修饰的人干扰素的研制A1A2A3A4A0A5A6A7A8A9A10A11A12A13A14A15A16A17A18A196HISA20A21A22A9A10A11A12A23A24A25A26A27A28A29A30A31PCRA32A33A34A35A36A37A38A39A40CDNAA41A42,A43A44A45A46A47PUC19,A48A41A49A50A51A52A53A54A55A45PQE80LA56A57A46A47A58A59A60BAMHIA61HINDIIIA62A63A64A53A59A6512A66A67A68A69A70A71A72A49A50A73A74A27SDSPAGEA61WESTERNBLOTA75A48A56A57A40A76A40A77A78,NI2NTAA79A80A81A77A78A59A63A82A83A84A85A86A48A50A80A81A40A76A40A77A78A87A88A89A90A91A92A93PCRA94A95A96A97A98A99A100A101A102A103CDNAA104A105A106A107A108A109A110A111A112,SDSPAGEA113WESTERNBLOTA114A115A116A117HISA118A119A120A100A101A102A103A121A122A123A124A125A126A127A96A128A129A130A131A132,SDSPAGEA133A134A135A120A136A137A138A139A140A129A141A142A106A143A144A145A146A147A148A149A150A151A152A153A154A155A156A157A158A159A154A160A161A162A163A164A165A166A167A168A169A170A1716HISA172A173A174A154A175A176A177A178A164A179A180A181A182A183A184A185A186A187A188A186A189A190A191A192KEYWORDSHUMANINTERFERONGAMMAEXPESSIONPROTEINPURIFICATIONA193A183A184A185A194HUMANINTERFERONGAMMA,HUINFA195A196A197A198A199A200A201A198A202A203A204A205A206A207A208A209A210A2111A212A213A214A215A216A217A218A211A219A220A221HUINFA222A223A224A225A226A227A228A229A230A231A232HUINFA233A234A235A236A237A235A238A228A239A240A241A242HUINFA222A243A244A228A245A242A246A247A248A249A250A251A235A236A237A235A238A252HISA253A243A244A254A255A103A0A1A2A228A93A3A4A5A229A6A241A242A228A14A76HISA16A8A222A9A10A11A12A141A230A142A243A244A228A245A242HISA253A243A244A13A15A232A142A235A236A237A235A238A228A95A17A18A10A11A12A222A225A226A19A254A252A147A20A245A242A21A22A19A23A24A25A228A1546HISA253A26A27A28A9A10A11A12A222NA156A118A228A14A76HISA16A8A222A9A10A11A12A2521A29A30A31A32A3311A35A34A36A37A38PUC19A109PQE80LA35A34A36DH5A37A38A39A158A40A41A42A43A44A45A4612A162A47A36A48A49TRIPURERNAA166A50A162A47A51ROCHEA168A52A53A54A39A170A55A56A57A58A59BAMHIA60HINDIII,DNAA172A61A62A63T4A173A64A62A65A66A67A173A68A69A70A71A72A73PTPCRA74A75A76A77A78A79PROMGAA71A72A80A81A63A82A83A84A85A74A75A76A86DNAA87A88A89A181A90A60PCRA80A70A181A90A74A75A76A77A78A79OMEGAA71A72A80A81A63A183A70A91A92A94A69A96A71A72A61A97A63A106HISA131A106A60NI2NTAA185A98A99A100A65A66A101A79QIAGENA71A72A63A189A102A104A105A107A108A110A111A112IPTGA77A78A79SIGMAA71A72A80A81A63A196A113A114A115A111A116A117A119A120A203A77A121A122A123A124A69A70A125A126A127A128A71A72A80A81A7313A183A70A129A130A132A61A97A77A133A134A135A136A137A138A139A129A87A88,A212A140A130A132A143A144A145A146A148A149A150A151A129HUIFNA183A70A73A92A86A218A152A183A70A153A155A157A159BAMHIA60HINDIIIA222A160A62A161A163A164A63A183A70A165A167A169A218A227A92A152A183A70A2275A228GTGGATCCCAGGACCCATATGTAAAAG3A228A63A218A152A183A70A2275A228GGCAAGCTTTTACTGGGATGCTCTTCA7314A171A131A160A116A229A174A175PBMCA176A231RNAA84A85A232A177A64A178A196A113A114A115A111A116A117A119A120A203A159A126A129A179A171A63IM36HA180A63A85A235A182A18410MLA134A186A187A188A190A191A19202MLA129A193A194A195A197,A198A159A199A200A229A174A153A193A203A153A193A131A160A116A229A174A63A248A84A85PBMCA231RNAA73A127A201A202A204A205A206RNAA84A85A74A75A76A207A208A202A204,A137A133A231RNAA209A210A21170A213A214A197A21570A216A217A210A7315PTPCRA219A220A144A145A221A223HUIFNA87A88A198A159PTPCRA224A225A144A145A226A221A223HUIFNCDNAA87A88A73PTPCRA219A220A230PBMCA231RNAA77A233A234A73A236A237A238A239A22745A216A240A241A24245MINA24394A2442MINA245A246A247A249A250TAQDNAA251A252A253A243A254CDNAA255A22A21A1A8A0,94A24430S,57A2441MIN,68A2442MIN,A2A127A335A223A10A4A243A23A4268A244A24A57MINA19A181A6RTPCRA135A7A47A9A11A9A12A13,A48BAMHIA49HINDIIIA50A253A14,08A26A15A225A16A17A18A20A25A27A28A42A25A181A6HUIFNA12A13A29A43A30A31PUC19A118A29A241A69A32A234A33DH5A229A34A29A35A66A36A37A221A29A38A221A66A36A131A39A40A41A44A45A46A42,A51A129A52A53A54A55A56,A127A3A50A253A14A57A58A292A59A60A101A61A159A55A56A229A34A62A63A1916PQE80L/HUIFNA64A65A67A68A9A43A30A70A62A63A71A72A9A61A159PUC19/HUIFNA55A56A48A51A129A52A53A54,A48BAMHIA49HINDIIIA50A253A14A42A16A17A18A27A28A25A181A6HUIFNA12A13,A43A30A73PQE80LA67A68A118A19A248A241A69A32A234A33DH5A229A34,A74A75A76A77A78A79A101A37A221A29A38A221A241A69A131A39A40A41A44A45A46A42,A51A129A52A53A54A55A56,A127A3A50A253A14A57A58A60A101A39A40A1917A80A66A9A81A82A64A65A38A54A83A61A159A84A199A64A65A67A68A9A131A223A34A85A41A44A86LBA45A46A203A47,37A244,225RMIN1A87A88A45A46A89A90,A233A91A2541A41A44A735MLLBA45A46A203A47,37A244,A87A88A45A46A92A600A106A93,A240IPTGA92A94A95A97A11MMOLL1,A96A98A45A464HA1918SDSPAGEA99A252A100A102A192A103A104A105A7A106A107A108A109A110A111A127A32,A25415A9A251A112A113A114A115A1A103A116A25,A95A117A25A95A97A15,A54A118A119A45A46A2031ML,A116A24310000G,1MIN,A120A121A240100A122L1A123A118A124A125A126A203,100A244A12810MIN,A5420A122LA118A124,A27A28A42,A130A132A133A206A134A136A137A138A13919WESTERNBLOTA140A141A142A143A144A145PQE80L/HUIFN/DH5A146A147A148A149A130SDSA172A150A151A152A153A154A155A156A157A158,A160A154A155A161A145A162A163A164A165A166A156A167A168A169A170A200A171A173A174A175A161,A176A177A178SA137A138A158A179A180MARKERA145A182A183,A214A184A185A186A187A214A175A161A145A176A177A178S,5A188A189A190A191A193A194A195A196,A197A198HISA201A198A202A204,A187A205A158A197HRPA179A207A145A208A209A198A210A193A194A202A2041H,A187A175A158A197DABA196A211A212A138A139110NI2NTAA213A215A216A141A217A218A219A220A222A162A163A160A224A220A222A226A227PQE80L/HUIFNA145A228A230A149A231500MLLBA232A235A236A237A238A239A166A24117,A184A242A244A245A149A210,A246A247A249A149,A184A242A250A251A253A254A255A210,A21A81A0A203A8MOL/LA123A174A10901MOL/LA124A200A209A112A1A109001MOL/LTRISHCLPH80A127A2,A127A2A161A129A3NI2NTAA216A141A217,A251A4A21A81A0A203AA215A81A0A203B8MOLL1A123A174A10901MOLL1A124A200A209A112A1A109001MOLL1TRISHCLPH63A187A217,A130A169A280A37A50001,A21A81A0A203CA81A0A203BA232A224025MMOLL1A131A6A187A188A64A145A162A163A13A20A7A133A23A134A8A123A174A62A97A145A239A166,A135A141A136A214A218A219A162A163A137A203A232A145A123A174,A121A138A101A162A163A231A139A3A148A232A151A140A9A101,A142A165A253A75HUIFNA13A130SDSPAGEA140A141A80A10A162A163A218A97A139111ELISAA63A11HUIFNA143A7A80A10A145HUIFNA144A12A13A21PBSA145A14A7571000PGML1A147A15A13A16A17A18A19A22A24ELISAA162A25A26A27A28A292A30A3121RTPCRA32A33A34HUIFNCDNAA76A3512A54A36A38A39A40A41A42A43A44A45480BPA149A95A46A150A189A47A48A49A50,A51A52A53A55A56A57A58A238A155A59A46A157A60A158A611A104A160A105A6522DNAA66A67A68A69A70A71A211A72HUIFNA73CDNAA66A67A74A2275CAGGACCCATATGTAAAAGAAGCAGAAAACCTTAAGAAATATTTTAATGCAGGTCATTCAGATGTAGCGGATAATGGAACTCTTTTCTTAGGCATTTTGAAGAATTGGAAAGAGGAGAGTGACAGAAAAATAATGCAGAGCCAAATTGTCTCCTTTTACTTCAAACTTTTTAAAAACTTTAAAGATGACCAGAGCATCCAAAAGAGTGTGGAGACCATCAAGGAAGACATGAATGTCAAGTTTTTCAATAGCAACAAAAAGAAACGAGATGACTTCGAAAAGCTGACTAATTATTCGGTAACTGACTTGAATGTCCAACGCAAAGCAATACATGAACTCATCCAAGTGATGGCTGAACTGTCGCCAGCAGCTAAAACAGGGAAGCGAAAAAGGAGTCAGATGCTGTTTCGAGGTCGAAGAG3CATCCCAGTAA3BLASTA161A126A77A78A56GENBANKA79A82A83A84A85A86A87CDNAA66A67A46A157A6523PQE80LHUIFNA88BAMHIA89HINDIIIA90A91A92A93A94A96A98A99A100A102A103A77A78A106480BPA8947KBA107A95A46A49A50A60A108A110PQE80L/HUIFNA111A159A113A114A115A88A163A116A117A118A119A6524A120A122A73A125A128A132A141PQE80L/HUIFNA113A114A73DH5A146A148A88IPTGA125A128A93A132A141A152A153A73A120A122A60A164A154A156A165A166A167A168A169A106A6525A120A122A73A170A69WESTERNBLOTA171A172A77A78,A88A125A128A73A148A166A173A174A175A56A176HISA177A176A178A179,A106A171A180A181A18217KDA149A183A184A177A46A49A50,A185A186A88A125A128A73A148A166A187A188A190A178A179,A253A110A191A192A956HISA153A73A120A122A193A194A132A141A60A158A612A104A160A105A6526A120A122A73A195A196A197A198A68A120A122A88A132A141A199A200A201A89NI2NTAA195A196A93A202SDSPAGE,A70A71A102A103A204A79A205A177A46A49A50,A253A110A153A73A120A122A254A95A206A207A
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