常用缓冲溶液的配制方法

常用缓冲溶液的配制方法磷酸氢二钠柠檬酸缓冲液pH0.2mol/L Na2HPO4（毫升）0.1mol/L柠檬酸（毫升）pH0.2mol/L Na2HPO4 （毫升）0.1mol/L柠檬酸（毫升）2.22.42.62.83.03.23.43.63.84.04.24.44.64.85.00.401.242.183.174.114.945.706.447.107.718.288.829.359.8610.3010.6018.7617.8216.8315.8915.0614.3013.5612.9012.2911.7211.1810.6510.149.705.25.45.65.86.06.26.46.66.87.07.27.47.67.88.010.7211.1511.6012.0912.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.459.288.858.407.917.376.786.155.454.553.532.611.831.270.850.55Na2HPO4分子量 = 141.98，0.2 mol/L溶液为28.40克/升。Na2HPO42H2O分子量 = 178.05，0.2 mol/L溶液含35.01克/升。C4H2O7H2O分子量 = 210.14，0.1 mol/L溶液为21.01克/升。pH 4.0 20mL：Na2HPO4 0.219g + C4H2O7H2O 0.258g柠檬酸柠檬酸钠缓冲液（0.1 mol/L）pH0.1 mol/L柠檬酸（毫升）0.1 mol/L柠檬酸钠（毫升）pH0.1 mol/L柠檬酸（毫升）0.1 mol/L柠檬酸钠（毫升）3.03.23.43.63.84.04.24.44.64.818.617.216.014.914.013.112.311.410.39.21.42.84.05.16.06.97.78.69.710.85.05.25.45.65.86.06.26.46.68.27.36.45.54.73.82.82.01.411.812.713.614.515.316.217.218.018.6柠檬酸C6H8O7H2O：分子量210.14，0.1 mol/L溶液为21.01克/升。柠檬酸钠Na3 C6H5O72H2O：分子量294.12，0.1 mol/L溶液为29.41克/毫升。pH 4.0 20mL： C4H2O7H2O 0.275g + Na3 C6H5O72H2O 0.203g乙酸乙酸钠缓冲液（0.2 mol/L）pH(18)0.2 mol/L NaAc(毫升)0.3 mol/L HAc(毫升)pH(18)0.2 mol/LNaAc(毫升)0.3 mol/LHAc(毫升)2.63.84.04.24.44.60.751.201.802.653.704.909.258.808.207.356.305.104.85.05.25.45.65.85.907.007.908.609.109.404.103.002.101.400.900.60Na2Ac3H2O分子量 = 136.09，0.2 mol/L溶液为27.22克/升。 pH 4.0 20mL：NaAc 0.098g + HAc 0.282mL甘氨酸氢氧化钠缓冲液（0.05M）X毫升0.2M（mol/L）甘氨酸+Y毫升0.2N（g/L）NaOH加水稀释至200毫升pHXYpHXY8.68.89.09.29.450505050504.06.08.812.016.89.69.810.010.410.6505050505022.427.232.038.645.5 甘氨酸分子量=75.07; 0.2M溶液含15.01克/升。 pH 10.0 20mL：甘氨酸0.075g + NaOH 0.013g碳酸钠-碳酸氢钠缓冲液（0.1M）Ca2+、Mg2+存在时不得使用pH0.1MNa2CO3(毫升)0.1MNa2HCO3(毫升)20379.169.409.519.789.9010.1410.2810.5310.838.779.129.409.509.729.9010.0810.2810.57123456789987654321 无水Na2CO2分子量=105.99;0.1M溶液为10.60克/升。 Na2CO210H2O分子量=286.2;0.1M溶液为28.62克/升。 Na2HCO3分子量=84.0;0.1M溶液为8.40克/升。 pH 10.0 20mL：无水碳酸钠 0.127g +碳酸氢钠0.067g补注：pH 4.0 EDTA2-McIlvaine2O 0. 05 mol/L EDTA（372.2）+ 0. 06 mol/L Na2HPO412HO（358.14） + 0. 08 mol/L 柠檬酸（210.14） 18. 61g/