细胞生物学第十章 细胞核（四）.ppt

Chapter10NucleiandChromosomes细胞核和染色体-4,MedicalCellBiology,JingChangqin(井长勤)associateprofessorE-mail：jingchangqin,2,SectionfourNucleolus（核仁）,3,4.1GeneralinformationofNucleolus,Structure:HavenomembraneNumber:oneormanyLocation:centerorverge,4,FibrillarcentersDensefibrillarcomponentGranularcomponentNucleolusMatrix,4.2structureofnucleolus,5,envelope,nucleolus,tenloopswithrDNAatinterphase,cutting,Partofnucleoluswithchromatinloops,4.2structureofnucleolus,6,Nucleolusorganizationregion(NOR),ChromosomesorganizingtoformnucleolusDgroup：13、14、15;Ggroup：21、22ContainingrDNA(rRNAgene),7,4.3functionofnucleolus,RibosomalBiogenesis:pre-rRNAsynthesis,Process,Assembly,FC.DFC,DFC.GC,Cytosol,Directionalprocess,8,CharacteristicsofRNAtranscriptionNORsinhumanchromosomes:1314152122Codefor(ineukaryotes):18s,28s,5.8srRNA,4.3functionofnucleolus,9,SynthesisandprocessingofrRNAprecursorCleavageofpre-rRNA,4.3functionofnucleolus,10,Synthesisandprocessingof5srRNA,5srRNAsareencodedbyalargenumbergenes(Human,2000)5srRNAgenearelocatedoutsidethenucleolus.5srRNAsaretranscibedbyRNApolyIII.The3endof5srRNAisremovedduringprocessing.,11,Theassemblyofribosomes,12,4.4nucleolarcycle,Thenucleolusdisappearsduringmitosis,13,4.4nucleolarcycle,14,Summaryfornucleolus,Generalinformationstructureofnucleolusfunctionofnucleolusnucleolarcycle,15,SectionfiveFunctionofNucleus（细胞核的功能）,16,6.1importanceofDNAandnucleus,AngelmansyndromePrader-Willisyndromedel（15）（q11-q13）Deletionfrommother,thenASDeletionfromfather,thenPWS,17,6.2DNAmainlylocatedinnucleus,GenomesizegenerallyincreaseswithanorganismscomplexityCvalueparadox,18,6.3DNAreplication,semiconservativereplicationsemidiscontinuousreplicationRNAprimerEnzymesandprotein,19,6.4DNAtranscription,SensechainandantisensechainProcessingofpre-RNARegulationoftheprocessoftranscriptionOperoncis-actingelementandtrans-actingfactor,Summary,NucleolusFunctionsofnucleus,20,21,Summaryfornucleus,PrefaceStructureNuclearenvelopeChromatinChromosomeNucleolusFunction,22,Exercise,1.Explainthekeywordsnucleolus2.Whatarethefunctionsofnuclei?,23,1.翟中和等主编.细胞生物学，第3版,高等教育出版社，20072.韩贻仁主编.分子细胞生物学(第3版),高等教育出版社,20073.艾伯茨等著，赵寿元等译.基础细胞生物学，上海科学技术出版社，20024.王金发.细胞生物学,科学出版社，20045.杨恬.细胞生物学，人民卫生出版社，20056.KarpG.CellandMolecularBiology,4thed(200