[生物医药论文精品]hplc法测定降酶片虎杖中白藜芦醇和虎杖苷的含量

HPLC法测定降酶片虎杖中白藜芦醇和虎杖苷的含量降酶片由虎杖、白花舌蛇草等中药组成。虎杖为蓼科属多年生草本植物，药用部位为根及根茎。虎杖始载于名医别录，列为中品，具有祛风利湿、散瘀定痛、止咳化痰等功效；用于关节痹痛，湿热黄疸，经闲，徵瘕，水火烫伤，跌扑损伤，痈肿疮毒，咳嗽痰多。虎杖中主要含蒽醌类、二苯乙烯类、黄酮类等多酚性化合物。我们采用HPLC法测定虎杖中白藜芦醇和虎杖苷，为降酶片的质量标准的制定提供了实验依据。本法专属性强，操作简G1427，G12946G4506G5242G20652，G18337G10628性G3921，G14033G7368G3921的G6523制降酶片的质量，G1457G19568G1032G5214G5224用的有效性及G12295定性。1G1214G3132G994G16809G2070UV2000G20652效G9094G11468G14406G16901G1214；SP8800TERNARYHPLCPUMPSPECTRAPHYSICS；虎杖苷G4557G10043品G708G6221G250711575200301G709，白藜芦醇G4557G10043品G708G6221G2507111535200301G709由中G3281药品生物制品G7828定G6164提供。降酶片由本G19510制G2070G4472提供，G6221G2507060820、060821、060822。2G7053法G994G13479G753621G14406G16901G2010G7524G7477G1226G726IRREGULARC18G7621G70846G104250MM5G173G709；G8981G2172G11468G726乙G14108G726水G70825G72675G709；G8981G17907G72610MLG103MIN1；G7621G9213G72635G263；G7828测G8886G19283G726306NM；G17839G7691量G72620G173L；G10714G16782G3624G7507G6980G1209虎杖苷G4804G16757G993G1314于3000，G1209白藜芦醇G4804G16757G993G1314于3000。22G9354G9094的制G3803221G4557G10043品G9354G9094的制G3803G12946G4506G12228G2474虎杖苷G4557G10043品804MGG13634100MLG4493量G10954中，G2164G11014醇G9354G16311G5194G12244G18334G14279G2063G5242，G6683G2260G5483G8999G5242为00804MGG103ML1虎杖苷G4557G10043品G9354G9094。G12946G4506G12228G2474白藜芦醇612MGG13634100MLG4493量G10954中，G2164G11014醇G9354G16311G5194G12244G18334G14279G2063G5242，G6683G2260，G5483G8999G5242为00612MGG103ML1的白藜芦醇G4557G10043品G9354G9094。222供G16809品G9354G9094的制G3803G2474G14270制降酶片20片，G11752G2260，G247450MG，G12946G4506G12228定，G1363450MLG4493量G10954中，G2164G11014醇G17878量，G17241G378030G2010G19059，G2164G11014醇G12244G18334G14279G2063G5242，G6683G2260，G12946G4506量G247410MLG1427910ML量G10954中，G2164G11014醇G12244G18334G14279G2063G5242，G6683G2260，045G173MG5506G4392G9400G14192G17819G9400，G13505G9400G9094G3803用。23标准G7366G13459的制G3803G726G12946G4506量G2474G8999G5242为00804MGG103ML1虎杖苷G4557G10043品G9354G90940ML、10ML、20ML、30ML、40ML、50ML于10ML量G10954中，G2164G11014醇G14279G2063G5242，G6683G2260，045G173MG5506G4392G9400G14192G17819G9400，G2474G13505G9400G9094，G6365G990G17860G14406G16901G7477G1226测定。G1209虎杖苷的G8999G5242为G8190G3364标G708X1G709，G1866G4804G19766G12227为G13449G3364标G708Y1G709，G13484制标准G7366G13459。G16757G12651G5483G1866G3250G5414G7053G12255Y138364300X1G25251941，R09999。虎杖苷G33240G794004020MGG103ML1G994G4804G19766G12227G2588G14403G3921的G13459性关G13007。G12946G4506量G2474G8999G5242为00612MGG103ML1的白藜芦醇G4557G10043品G9354G90940ML、10ML、20ML、30ML、40ML、50ML于10ML量G10954中，G2164G11014醇G14279G2063G5242，G6683G2260，045G173MG5506G4392G9400G14192G17819G9400，G2474G13505G9400G9094，G6365G990G17860G14406G16901G7477G1226测定。G1209白藜芦醇的G8999G5242为G8190G3364标G708X2G709，G1866G4804G19766G12227为G13449G3364标G708Y2G709，G13484制标准G7366G13459。G16757G12651G5483G1866G3250G5414G7053G12255Y285777166X2G252227248，R09999。白藜芦醇G33240G794003060MGG103ML1G994G4804G19766G12227G2588G14403G3921的G13459性关G13007。24G12366白实验G12946G4506G12228G2474G6365制G3803G5049G14414制G3803的G2376虎杖的G19464性G7691品167MGG6365222供G16809品G9354G9094的制G3803G7053法制G3803。G19464性G7691品HPLCG14406G16901中，G3324虎杖苷、白藜芦醇G4804处无G4804，表明G19464性G7691品G993干扰测定。25G12946G4506G5242G16809验G2474虎杖苷标准G7366G13459项下G8999G5242为002412MGG103ML1G4557G10043品G9354G9094，G6365G990G17860G14406G16901G7477G1226，G18337复G17839G76916次，G16757G12651RSD036。G2474白藜芦醇标准G7366G13459项下G8999G5242为001836MGG103ML1的G4557G10043品G9354G9094，G6365G990G17860G14406G16901G7477G1226，G18337复G17839G76916次，G16757G12651RSD087。26G12295定性G16809验G2474G6221G2507为060820G7691品，G6365222项下供G16809品G9354G9094的制G3803G7053法，G6365G990G17860G14406G16901G7477G1226G2010别于0、2、4、6、8小时G17839G7691，测定。测G5483虎杖苷G4804G19766G12227的RSD007；白藜芦醇G4804G19766G12227的RSD056，表明本品G33248小时内测G5483的G13479G7536是G12295定的。27G18337G10628性G16809验G2474G6221G2507为060820G7691品，G6365222项下供G16809品G9354G9094的制G3803G7053法制G3803，G6365G990G17860G14406G16901G7477G1226测定，测G5483虎杖苷、白藜芦醇含量。测G5483虎杖苷平均含量为2422，RSD105。白藜芦醇平均含量为1861，RSD142。28G2164G7691G3250收率G16809验G2474G6221G2507为060821G7691品，G12946G4506G12228G247425MG，G18337复5份，G2010别G2164虎杖苷G4557G10043品605MG，白藜芦醇G4557G10043品465MG，G6365222项下供G16809品G9354G9094的制G3803G7053法制G3803，G6365G990G17860G14406G16901G7477G1226测定，G16757G12651G3250收率。测G5483虎杖苷平均G3250收率为10044，RSD167；白藜芦醇平均G3250收率为10102，RSD186。29G7691品含量测定G2474G6221G2507060820、060821、060822的3G6221G7691品G708每G6221平行3份G709，G6365222项下供G16809品G9354G9094的制G3803G7053法制G3803，G6365G990G17860G14406G16901G7477G1226测定。表1降酶片中虎杖苷含量测定的G13479G7536G708N3G709TABLE1ANALYSISOFCONTENTOFA1A0A2A0A3A4INJIANGMEITABLETN3G6221G2507虎杖苷的含量MGG103G1RSDG708G709060820242504906082124480320608222423030表2降酶片中白藜芦醇含量测定的G13479G7536G708N3G709TABLE2ANALYSISOFCONTENTOFA6A0A5A7A0A8A9A10A11A12A3A13A14A3A9A13A15A16A0A3A17A9A18A12A0A10A21A13A19A20A24G6221G2507白藜芦醇的含量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讨G1678231一般白藜芦醇和虎杖苷都G14033同时存G3324于植物中，二者G3324一定外界G7477G1226下会G11468互转化。目前的G11752究表明反式异构体是G1866活性成G2010，G10628一般测定反式的白藜芦醇和虎杖苷的总量作为评价标准。G3324葡萄及葡萄酒中同时发G10628了四种异构体，而目前虎杖中的顺式白藜芦醇及G1866苷鲜见报道。因此用反式白藜芦醇及虎杖苷为指标性评价虎杖质量是很有必要且合G10714的。32本文建立了用HPLC