纳米银自催化氧化测定过氧苯甲酰的光谱研究 - nature

1、A new silver nanorod SPR probe for detection of trace benzoyl peroxideZhiliang Jiang*, Guiqing Wen, Yanghe Luo, Xinghui Zhang, Qingye Liu, Aihui Liang*(Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin 54100

2、4, China)Key words: silver nanorod; stable nanosol; SPR; Rayleigh scattering; SERS; BPO; detection. AfgabcdeWavelength/nmFigure 1S Absorption spectra of the AgNPB-NaCl-BPO systema: 5.010-5 mol/L AgNPB -6.2510-4 mol/L NaCl-pH 6.2; b:a-0.4g/mL BPO; c: a-1.6g/mL BPO; d:a-3.2g/mLBPO; e:a-4.0g/mL BPO; f:

3、a-6.4g/mL BPO; g:a-7.2g/mL BPO.Aa b c d e f g habcdefghWavelength/nmFigure 2S Absorption spectra of the AgNR-NaCl-BPO systema: 5.010-5 mol/L AgNR -5.010-4 mol/L NaCl-pH 6.2; b:a-0.5g/mLBPO; c: a-1.5g/mLBPO; d:a-2.0g/mLBPO; e:a-2.5g/mLBPO; f:a-3.0g/mLBPO; g:a-3.5g/mLBPO; h:a-4.0g/mLBPO.abcdefFigure 3

4、S RRS spectra of the AgNR-BPO systema: 50mol/L AgNR; b:a+0.4 mg/L BPO-pH 6.2; c:a+2 mg/L BPO; d: a+ 4 mg/L BPO; e: a+8 mg/L BPO; f: a+10mg/L BPO.IfedcbaWavelength/nmFigure 4S RRS spectra of the AgNPB-NaCl-BPO systema: 5.010-5 mol/L AgNPB-6.2510-4 mol/L NaCl-pH 6.2; b:a-0.8mg/L BPO; c: a-1.6mg/L BPO;

5、 d:a-2.4mg/L BPO; e:a-3.2mg/L BPO; f:a-4.0mg/L BPO.hgfedcbaI Wavelength/nmFigure 5S RRS spectra of the AgNR-NaCl-BPO systema: 5.010-5 mol/L AgNR -5.010-4 mol/L NaCl-pH 6.2; b:a-0.5g/mLBPO; c: a-1.0g/mLBPO; d:a-1.5g/mLBPO; e:a-2.0g/mLBPO; f:a-2.5g/mLBPO; g:a-3.0g/mLBPO; h:a-3.5g/mLBPO.Iabcde Raman sh

6、ift/cm-1Figure 6S SERS spectra of the AgNR-NaClBPO-VBB systema: 5.010-5 mol/L AgNR -5.010-4 mol/L NaCl-4.010-7 mol/L VBB-pH 6.2; b:a-0.4g/mLBPO; c: a-0.8g/mL BPO; d:a-1.5g/mLBPO; e:a-2.0g/mLBPO.abcdIRaman shift/cm-1Figure 7S SERS spectra of the AgNR-BPO-VBB systema: 5.010-5 mol/L AgNR -4.010-7 mol/L

7、 VBB-pH 6.2; b:a-1.0g/mLBPO; c: a-2.0g/mLBPO; d:a-3.0g/mLBPO. abIM VBBFigure 8S Effect of VBB concentration on I5.010-5 mol/L AgNR-5.010-4 mol/L NaCl-4.010-7 mol/L VBB-0.4g/mLBPO-pH 6.2a: VBB was added before reaction; b: VBB was added after reaction.a b c dFigure 9S Colors of the four AgNP solsa: 0

8、.1 mmol/L AgNO3-3.6mM sodium citrate -0.072% H2O2-0.4 mM NaBH4;b: a after heating at 100 water bath for 10min；c: 0.1 mmol/L AgNO3-0.6mM sodium citrate -0.072% H2O2-0.4 mM NaBH4; d: c after heating at 100 water bath for 10min.AI Wavelength (nm)Figure 10S RRS and absorption spectra of AgNR (n=5)25mol/

9、L AgNR.abIM AgNPFigure 11S Effect of AgNP concentration on Ia: AgNR-5.010-4 mol/L NaCl-4.0g/mL BPO-pH 6.2; b: AgNPB-6.2510-4 mol/L NaCl-4.0g/mL BPO-pH 6.2.abImM NaClFigure 12S Effect of NaCl concentration on I of AgNR and AgNPB systema: 5.010-5 mol/L AgNR-4.0g/mL BPO-pH 6.2; b: 5.010-5 mol/L AgNPB-4

10、.0g/mL BPO-pH 6.2.abIT/Figure 13S Effect of reaction temperature on Ia: 5.010-5 mol/L AgNR-5.010-4 mol/L NaCl-4.0g/mL BPO-pH 6.2; b: 5.010-5 mol/L AgNPB-5.010-4 mol/L NaCl-4.0g/mL BPO-pH 6.2.Iab t/minFigure 14S Effect of reaction time on Ia: 5.010-5 mol/L AgNPB-5.010-4 mol/L NaCl-4.0g/mL BPO-pH 6.2;

11、 b: 5.010-5 mol/L AgNR-5.010-4 mol/L NaCl-4.0g/mL BPO-pH 6.2.abImg/L BPOFigure 15S Working curve for AgNP-NaCl RRS method a: 5.010-5 mol/L AgNR-5.010-4 mol/L NaCl-pH 6.2; b: 5.010-5 mol/L AgNPB-6.2510-4 mol/L NaCl-pH 6.2.abAmg/L BPOFigure 16S Working curve of SPR method of AgNP-NaCl system a: 5.010-

12、5 mol/L AgNR-5.010-4 mol/L NaCl-pH 6.2; b: 5.010-5 mol/L AgNPB-6.2510-4 mol/L NaCl-pH 6.2.Figure 17S Working curve of SPR method of AgNR-BPO system 50mol/L AgNR-pH 6.2baIIg/mL BPOFigure 18S Working curve of SERS method of AgNP-NaCl system a. 5.010-5 mol/L AgNR -5.010-4 mol/L NaCl-4.010-7 mol/L VBB-p

13、H 6.2 ; b. 5.010-5 mol/L AgNR -4.010-7 mol/L VBB-pH 6.2.abcdABdcbaT% Figure 19S Extinction spectra (A) and RRS spectra (B) of AgNRa: 5mol/L AgNRs; b:12.5mol/L AgNRs; c: 25mol/L AgNRs; d:50mol/L AgNRs.Figure 20S Principle of the SERS enhancement mechanism of aggregated nanorodsTable 1S Analytical per

14、formance of some reported methods for BPOMethodPrincipleLR(g/mL)DL(g/mL)CommentsRef.Fluore-scenceBPO oxidizes Fe2+ into Fe3+ that induces the opening of the spirolactam ring of N-methoxy rhodamine-6G spirolactam, switching on fluorescence of the detection system.0.2-3.20.06Sensitive23Chemilum-inesce

15、neThe determination of benzoyl peroxide was based on a capillary microliter order droplet sample jectionluminol chemiluminescence system. 0.0005-10.00014Highly sensitive, but complicated.24Electro-chemicalCarbon paste electrode contained fibers of coconut fruit, which are very rich in peroxidase enz

16、ymes naturally immobilized on its structure.1110-12210555Low sensitivity.25Chromato-graphyBPO was determined as benzoic acid after preceding reduction by potassium iodide.0.5-300.29Low sensitivity.26Fluoresce-nce BPO oxidized the probe to form strong fluorescence0.12-6.30.06Selective.27RRSBPO oxidized AgNR to form big particles with strong RRS effect. 0.