紫外-可见漫反射光谱

1、紫外-可见漫反射光谱 原理及操作,March 20, 2021,diffuse reflection spectrum,一. 漫反射光谱原理,二. 漫反射光谱与积分球,三. 紫外-可见漫反射光谱仪器原理及操作,主要内容,四. 紫外-可见漫反射趣味实验,镜面反射,漫 反 射,反射角=入射角,K 为吸收系数，S 为散射系数，R 表示无限厚样品 的反射系数R 的极限值,KubelkaMunk 方程式,一.漫反射光谱原理,漫反射光谱简介,紫外分光光度计与紫外漫反射的区别,后者：采用漫反射的方式（积分球），所测样品为固 体、粉末、乳浊液和悬浊液,前者：采用透射方式 ，所测样品为溶液,漫反射光是指从光源发

2、出的光进入样品内部，经过多 次反射、折射、散射及吸收后返回样品表面的光,二.漫反射光与积分球,The characteristics of typical integrating sphere coatings,Sample有吸收 反射量减少,PTFE（聚四氟乙烯）和一般漫反射材料（BaSO4）的反射率比较,System Hardware,三. 紫外-可见漫反射光谱仪器及操作,System Layout,Cuvette,Flash Xenon,Thickness Sheet Sample Holder,Fiber Connector,BTC611 Spectrometer,Accessorie

3、s,Transmission Test,1. Open the sliding cover on the top,1,2,3,2. Loose two screws and open the cover on cuvette holder,3. Insert the cuvette with reference material into the cuvette holder,4. Replace the cuvette with sample material,4,Reflectance Test,1,2,3,1.Put the port reducer on the sampling po

4、rt,2.Put the light trap on the sampling port -Dark scan,3. Put the Reference Standard on the sampling port Reference Scan,4,4.Take off reference and put the sample material on the sampling port,3.Plug and screw the fiber coming from the light source into the SMA connector,1.Take off the port plug or port reducer,2.Install the fiber adapter on the sampling port,Fiber Coupling,Reflectance Test: Check Transmission Test: Uncheck Fiber Coupling: Uncheck,1.Dark scan,2.Reference scan,3.Acquire one spectrum,Software Operation,不同颜色叶子的UV-vis漫反射光谱图,四. 紫外-可见漫反射趣味实验,比比谁的手更白,