医学检验技术资格考试复习资料（Medical laboratory technology qualification exam review materials）

1、医学检验技术资格考试复习资料（Medical laboratory technology qualification exam review materials）1. penicillin can inhibit the synthesis of peptidoglycan in cell wall;2. ordinary medium: contains the most basic nutrients needed by the bacteria, for most of the growth of bacteria; blood culture contains rich nutrien

2、ts for fastidious bacteria growth; SS culture may be selected for separation of bacterial growth inhibition and other bacterial growth medium; anaerobic reductive substances, reduce the oxidation reduction potential of the medium, conducive to the growth of anaerobic bacteria, which is not conducive

3、 to the growth of facultative anaerobic bacteria;3. drug sensitive test dilution method is used to quantitatively determine the MIC and MBC of the tested bacteria;4. bacterial culture methods commonly used in clinical bacteriological examination are aerobic culture, carbon dioxide culture and anaero

4、bic culture;The recommended 5. WHO syphilis serological diagnostic screening test is a rapid plasma reagin test (RPR); WHO confirmatory test recommended serodiagnosis of syphilis is fluorescent treponemal antibody absorption test (FTA-ABS);GLU and lactose decomposition of 6. Escherichia coli in KIA,

5、 gas production, do not produce H2S, MIU, dynamic positive, indole positive, urease negative in IMVIC test +- (adds);7., the three characteristics of parasitic diseases are endemic, seasonal, and some natural foci;8., five major parasitic diseases, schistosomiasis, malaria, Kala Azar, hookworm and f

6、ilariasis, have been extensively carried out in china;9., examination of the production of thick film should pay attention to: should be in the evening after nine oclock blood sampling, take three drops of blood, should be natural drying, blood test season should be warm in summer or when the climat

7、e is appropriate;10. can cause diarrhea protozoa are: Balantidium coli, Mao Dichong, Cryptosporidium, Giardia lamblia;11., the quality of Wright dye can be used absorbance ratio (RA) as Wright dye quality specifications, when Wright dye mature, its RA value should be close to 1.3;The main difference

8、 between the 12. tumors of the digestive tract and digestive tract ulcer patients with fecal occult blood test in positive duration;13. basophilic stippling erythrocyte formation: residual cytosolic RNA degeneration;14. IgM increased in cerebrospinal fluid, mainly in purulent meningitis;15. abnormal

9、 sperm morphology more than 30% is abnormal, can affect the pregnancy;16. cholesterol crystallization can be found in: bile, pleural effusion, joint cavity effusion, feces;17. the substance that slows ESR in serum is albumin and lecithin;18. precipitation reaction phase 1: antigen antibody specific

10、binding, complete in seconds to several minutes, available scattering turbidimetry reaction results;19. the methods of test tube agglutination test include: the test of obesity, the external Fiji test and the cross matching test of blood transfusion;20. positive indirect agglutination test is to det

11、ect the corresponding antibodies with known antigens, and the clinical is mainly used to detect antibodies to patients with autoimmune diseases.21. the detection of circulating immune complex is an auxiliary marker for the diagnosis of autoimmune diseases;Twenty-twoThe color at the end of the test h

12、ole shows a positive correlation with the antigen or antibody to be detected in the sample: double antibody sandwich method, double site one-step method and indirect method for the detection of antibodies;23. HbF examination, after 2 years of age, the correct value is less than 2.5%;24. normal peopl

13、e do not have red blood cells: HbH and HbS;25. in the normal coagulation process, complex shape can include: A:III, VIIa and Ca2+; B:IXa, VIIIa, C:Xa, Va, Ca2+ and phospholipids; phospholipids and Ca2+; etiological factor of 26. intrinsic coagulation pathway is the exogenous factor XII, factor III (

14、tissue factor);27. the main factors that determine the prolongation of bleeding time are platelet count and dysfunction;28. prolonged bleeding time is seen in thrombocytopenic purpura;29. factors sensitive to thrombin are I, V, VIII, and XIII;30. blood sampling, posture, exercise, age, sex, food may

15、 affect the biochemical components of the blood non pathological factors;The diagnostic efficiency of the 31. diagnostic test is the percentage of all the correct results in the experimental results;32. zone electrophoresis: filter paper electrophoresis, agarose electrophoresis, PAGE electrophoresis

16、, agar electrophoresis, and cellulose acetate membrane electrophoresis;33. factors affecting enzyme activity include substrate, sample storage time, temperature, pH value, buffer concentration;34. emission spectrometry is the principle of flame photometry;35., the phenomenon of Ding Li is found in:

17、scattering spectrum analysis;36. the maximum speed of the high speed centrifuge can reach 25000 revolutions per minute;37. gel chromatography: used to study the sedimentation characteristics of biological macromolecules and the concentration of dilute solutions;38. the main physiological functions o

18、f albumin in plasma proteins are nutrition, transport, maintenance, colloid osmotic pressure and buffering effect;39. urine beta 2 microglobulin is mainly used for renal function monitoring after renal transplantation; rejection of renal tubular function such as reaction effect, urine beta 2 microgl

19、obulin may be increased; in all nucleated cell surface; acute leukemia with neural invasion, CSF beta 2 microglobulin or higher;40. albumin is involved in regulating the metabolism of hormones and drugs, maintaining plasma colloid osmotic pressure, buffering the ability of acid and alkali, and is th

20、e main carrier of blood, accounting for 4060% of plasma proteins.41. the technique of combining zone electrophoresis with two-way agar diffusion is called immunoelectrophoresis;42. the identification of primary and secondary fibrinolysis fibrinolytic 3P is the most valuable test (3P test has better

21、specificity of secondary fibrinolysis but poor sensitivity, so the current proposed two plasma D- dimer detection instead of);43. CD41 positive acute leukemia of the primitive cell belongs to AML - M7 of FAB classification;The greatest advantage of the 44. method for the determination of urinary pro

22、tein is its high sensitivity;45. formaldehyde is often used as a preservative for the visible components of urine;46. oral anticoagulant PT prolongation, hemophilia APTT prolongation;47. enzymes used in the determination of serum cholesterol are cholesterol esterase, cholesterol oxidase and peroxida

23、se;Forty-eightPhosphotungstic acid magnesium reagent can selectively precipitate VLDL and LDL;49. the main characteristics of Enterobacter include fermentation of glucose, reducing nitrate, aerobic or facultative anaerobic and catalase positive;50. is used for the identification of Streptococcus gro

24、up B test: CAMP test and sodium hippurate hydrolysis test;51. plasma lipids include: cholesterol and cholesterol esters, phospholipids, glycerol three fat, free fatty acids;52. the transport of endogenous cholesterol to lipoproteins is mainly LDL;53. VLDL contains triglycerides, cholesterol, phospho

25、lipids, and cholesterol esters. It is the second triglyceride rich lipoprotein in the blood. It is synthesized in the liver; ApoCII activates LPL and promotes metabolism of VLDL in;54. after the pre marked lipoprotein agarose electrophoresis, the region of the cathode lipoprotein was CM, beta lipopr

26、otein, pre beta lipoprotein, and alpha lipoprotein;55. the indicator of metabolic acid-base balance in blood gas analysis is HCO3-;56. actual bicarbonate (AB) is equal to standard bicarbonate (SB), and the two is less than normal, indicating metabolic acidosis;57. alpha fetoprotein is not the larges

27、t molecular weight protein in plasma, 80% of patients with primary liver cancer serum content increased, mainly in fetal liver synthesis, used for fetal prenatal monitoring, not involved in the transport of substances;58. oncogene products include growth factors, P12 protein, DNA binding protein, pr

28、otein kinase, and not including chemical carcinogens;59. the smallest units of microbial classification are: species;60. medium sized bacillus: 23um 0.30.5um in length;61. The common component of cell wall of gram positive bacteria and gram negative bacteria is peptidoglycan;The 62. plasmid is a dou

29、ble stranded closed loop DNA molecule outside the bacterial chromosome;63. in the clinical bacteriology test, the indirect particles in the cytoplasm of a cell can be used for the identification of bacteria;64. in clinical bacteriology tests, the specific structures of bacteria used to identify bact

30、eria are capsules, spores, flagella;65. the main problem of Plasmodium with thick blood film is that it can change the morphology of plasmodium;66. typical episodes of malaria are periodic chills, fever, and sweating;Biochemistry of 67. grass green streptococci: negative enzyme test, optochin sensit

31、ive test, drug resistance, bile test negative;68. Streptococcus pneumoniae biochemical tests should be negative: negative enzyme test, sensitive optochin test, and positive bile test;69. of the parasites that can be infected by blood transfusion are malaria;After 70. tapeworm infection, can discharg

32、e the egg in excrement and urine, is broad section crack tapeworm.Culex pipiens pallens is the major vector of the 71. population;The rate of bone marrow examination in 72. Kala Azar is higher;73. mansoni mansoni and Taenia solium are both intermediate host and final host;74. Echinococcus granulosus

33、 people are intermediate hosts;75., the parasites damage to the host includes: mechanical, chemical, nutrient intake, immune response, and hypersensitivitySeventy-sixThe decrease in glucose levels in CSF can be seen in purulent meningitis, tuberculous meningitis, and Cryptococcus neoformans meningit

34、is;77. ABO blood group natural antibody mostly is IgM, the Immunity Antibody mostly belongs to IgG, the incomplete antibody can pass the placenta;78. agglutination is a granular antigen and the corresponding antibody forms the naked eye agglutination in the presence of suitable electrolyte;79. metho

35、ds for detecting antigens with known antibodies are ELISA (Jia Xinfa) and immunofluorescence (direct and indirect) methods;80. PAI (plasminogen activator inhibitor) is beneficial for hemostasis;81. serum ferritin is the most sensitive method of determining iron storage in the body;82. platelet aggre

36、gation factor (PAF) has the function of promoting platelet aggregation;83. the specific products in platelet alpha granules are beta -TG and PF4;84. the laboratory index of leukemia relapse is the bone marrow smear + the immature cell is more than 20%;The diagnostic efficiency of the 85. diagnostic

37、test is a percentage of all the correct results in the experimental results;86. the earliest electrophoretic technique used was free electrophoresis;87. zone electrophoresis consisted of filter paper electrophoresis, agarose gel electrophoresis, PAGE electrophoresis, agar electrophoresis, and cellul

38、ose acetate membrane electrophoresis;88. emission spectrometry is the principle of flame photometry;89. hypothyroidism manifested as unresponsive, bradycardia, hypogonadism, and non pitting mucinous edema;90. the indicator of metabolic acid-base balance in blood gas is HCO3-.91. the morphological id

39、entification of L1 and L2 in acute lymphoblastic leukemia mainly includes cell size, cytoplasm, nucleus shape and nucleolus;92. POX staining of bone marrow progenitor cells can be negative: acute myeloid leukemia, acute monocytic leukemia, acute myelomonocytic leukemia, acute megakaryocytic leukemia

40、, acute lymphocytic leukemia;The 93. electrode is the glass electrode pH meter;94., the pathogenic bacteria of cold increasing bacteria are: producing Lester cell of monocyte;95., according to epidemiological investigation, the main pathogenic Neisseria gonorrhoeae is A, B, C and Y groups, and the p

41、revalence of A group is the main in Europe and America, and the epidemic is mainly B and C group;96. according to different diseases, different specimens were separated and cultured, and the blood and urine were collected first, second weeks after typhoid fever for second, third weeks. The positive

42、rate of bone marrow isolation was high in the whole course of the disease;97. Borrelia burgdorferi is the cause of Lyme disease, and the major vector is ixodes;The 98. relapsing fever helix, which is transmitted by ticks, causes epidemic relapsing fever;99., the sulfur Leptospira, which is transmitt

43、ed by ticks, causes endemic relapsing fever;100. Shigella sonnei slow decomposition of lactose, serotypes belonging to the D group, only one serum type, its colonies often appear smooth colony, accompanied by a large, flat, rough colony;101. the common techniques for the determination of drug concen

44、tration include spectrometry, chromatography, radioimmunoassay, fluorescence immunoassay, and enzyme immunoassay;One hundred and twoElectrophoresis, which combines gel electrophoresis with immunochemical methods, is a transfer electrophoresis;103. cytotoxic T cells belong to the CD8+ subgroup;104. T

45、he site where the immunocompetent cells develop, differentiate and mature is the bone marrow and thymus gland;105. the major roles of activated protein C are inactivation of FVa and FVIIIa;106. platelet membrane glycoprotein IIb/IIIa complex is related to platelet aggregation function;107. may be Ka

46、posis ring formation in the cytoplasm of denatured lipoprotein or spindle caused by residue;108.1 liters =103 ml =106, slightly =109 Na litre, =1012 leather litre, =1015 soaring;The 109. FG is considered to be the most powerful erythropoietin rouleau formation material;110. gastrin is often characte

47、rized by elevated BAO;The 111. PT test reflects the levels of prothrombin, fibrinogen, factor V, VII, and X;112. ISI (International Sensitivity Index): the lower slope is more sensitive, said reagent standard organization and enzyme activity of each batch of tissue thromboplastin PT correction curve

48、, provided by the manufacturers;113., the bodys immune defense function is too high, susceptible to hypersensitivity;Principle 114.: horseshoe crab limulus test is a marine arthropod animal, the blood has a unique nuclear deformation of red blood cells, red blood cell lysate deformation can the aggl

49、utination reaction and bacterial endotoxin trace, which is due to an enzyme in cell lysate by bacterial lipopolysaccharide activated protein gel formation;115. the 154. Felix test principle: deformation of some special strains of the genus Bacillus bacteria such as X19, X2, XK antigen and some commo

50、n antigens of Rickettsia, can replace Rickettsia as antigen and some rickettsial diseases on serum for agglutination, to assist in the diagnosis of certain rickettsial diseases;116. the test of exogenous coagulation pathway is PT;117. glycogen staining is strongly positive in cells that are primary

51、megakaryocytes;118. principle of urine dry chemistry test: RBC and HGB were determined by four methyl benzidine, and the urine was determined by azo coupling method;119. can cause the reduction of phosphorus in the blood: hyperparathyroidism, vitamin D deficiency, renal tubular acidosis;120. the ind

52、ications of bone marrow examination are primary thrombocytopenic purpura, secondary thrombocytopenic purpura, leukemia accompanied by mucocutaneous hemorrhage.121. the biochemistry and metabolism of neutrophils include chemotaxis, conditioning, deformability and adhesion, bactericidal action, and ph

53、agocytosis;122. the pathogenesis of jaundice: excessive destruction of red cells, decreased bilirubin uptake by liver cells, decreased bilirubin treatment by liver cells and obstruction of biliary tract;123. pathogenic strains of Neisseria meningitidis meningitis is, on the dry and cold and heat sen

54、sitive;124. prokaryotic bacteria without cell walls are mycoplasma.125. can be used for capsule swelling test, typing bacteria: Streptococcus pneumoniae126. the Vi antigen of Salmonella belongs to surface antigen;One hundred and twenty-sevenThe pathogen of chancroid Haemophilus is: Duke;128. ELISA i

55、ndirect and indirect immunofluorescence can detect a variety of markers with a marker;The 129. E rosette test is a test of T cell counts by detecting SRBC receptors;130. in complement fixation tests, antibodies in patient sera can be detected;131. bronchial asthma belongs to type I allergy;132. one-way agar diffusion test sensitivity: not high, detected between 4872 hours;133. FVII deficiency: normal APTT, prolonged PT, plus normal plasma can correct;134. types of