奈米技术平台建构与生物晶片技术整合应用分项计划

1、development and application on the nano bio-chip technology for environmental inspection920411921210 (02)86625933 () 0928899487152(02) 86625933e-mail (:klin mail. tn it. edu.tw54mn2.2-12.2a.b.c.d.a.limi 100 mn192b.c.2a.b.d.23a.b.8003,000c.3c3.k-122002dnaic dramdna7185biochipsgene chipprotein chipcel

2、l chiptissue cliip55hiunan chipsensing chipmicro-fluidic biochip electropluesis chipsample preparation chip combinatory cheniistiy on a cliipdna niicroanay universal chipsequencing by hybridization6-8 mers affymetrix + hyseq standardized chipvliole-genome-on-a-chip n-mers custom-made chip6.(enviromn

3、ental bioteclmology)(bioremediation)(1)58b.(pop)(pah)(h2s)c.(bac)(a)codpilot-plant(b) (87)lab.-scalescale-upph8711a/ocoda/o16s rdnafluorescence159iii-situ hybridization, fishdnadna(pcr)restriction enzymeelectiophoresis, dgge)dnarrnacloningdna cloning (denatiue gradient gelcommunity finger printpcrpc

4、rcloningdna7.gene probe rt-pcr 16srrna-based dan-chipa. toxicantscdch bap tcedna-cliip pcr 24 26b. / on time/in-sitedenitrification no)一gpcr primer nirk and nirs probes rt-pcr 8 11c. water blooms toxin-producing bacteria cyanobacteriarrna-targetgene expression rt-pcrd. /mnadnapls/rt-pcr 9e bioremedi

5、ationhg+2lnaplsf.gem62gems gebs12,13g. pmn pre-manufachwe notice gems14h. biphenyl dioxygenases bp doxsnaphthalene doxs dioxin doxsm vitro dna-shufiling 19,20l/gieen chemistiybdsdna-shuffling 19t2j.dna-chip pcr genotoxic effects 22 27k.biosensor whole-cell biosensor dna-biiiding assays 28t29 30(21)6

6、3*:2(2)()a. pa (polyvinyl alcohol, pa)(alginate)paapsb ( penneable support bioreactor )mfsb ( membrane feeding substratebioreactor )psbmfsbpsb mfsbcodcod75% 80%45%2.5 g/m2/d 10 g/m2/d as caco3phg/m2/d3.6714.68 g/m2/d2.05.0hrt 2240%7:372codf99.3% 4:696codf99.1%9672hrt 750%4600 nil/min2.8 mg/l codf92%

7、68a/oorccod1,500 2,000mg/l750l.ooomg/lhrt 2 1(1) hrt = 3.0, 9.0, 12.0 dhrt 20 6.0, 8.0 d 1.0. 3.0, 4.0 d= 0.330.720.12 0.29 mg-cod/mg-cod d(4)=0.134.710.47 1.93 mg-cod/mg-cod d=00.22.0(6)mlss= 30004500ph6.08.04.0 mg/l30004000 mg/lph5.0pcb5.0(b)pcbspcbpcb(bac)quinonec/n c/pquinone1 .oppm 200 400ppbpc

8、bphphubiquonone menaquinonecod(87)lab.-scale scale-up pilot-plant9.07.09.07.011ph8711coda/oa/o692.3.2.693.70(92)(93)dna(94)(95)7116s rdna 16s rrnafish dggefishpcr16s rrna73fishdggepcrdntp2dnadnadna16s rdnadggedna(polymerase)dnapcr(primer)dnapcrpcr914-1924.1ss899091921501401221048415141414121. 4-2746

9、6223413/3/3/4/243/3/3( )3/3( )b.765.78a.1.2.3.4.b.1.2.3.811.2.3.a.1.2.3.b.1.2.92931.1-34-67-910-122.806-2242.56-118006-26-46-1( )1( )6-212,280,0002,280,0002,280,0002( )()145,00045,00045,0003( )1100,000100,000100,0002,425.000()13,000x230*1230dna20,000x260*1260-18 c50*150pcr230*1230dna40*14050*2100180

10、*1180dna70*2140( shaker)90*1905 l210*1210uv180*2360dna5 cross-linker50*150fraction collector140*114090*2180dna40*1402300632,300,0002,000,000300,0006-23,400,0003,400,00006-411,600,0008,600,0003.000,0006-517,300,00014,000,0003,000,000826-513,000x250*1250dna20,000x300*13004 c50*2100-18 c50*2100pcr300*1300dna40*28050*2100200*1200dna200*120070*2140( shaker)100*22005 l250*1250ut200*2400dna5 cross-linker50*2100fraction collector150*2300100*3300dna40*2803,4002,500*12,5003,000*13,000dna60