AbbreviationLCHVAA

1、program of study:general medicinecourse:biochemistryabbreviation:lch/vaa21schedule:45 hours of lectures60 hours of exercisescourse distribution:2nd year, 3rd semesternumber of credits:0course form:lectures, exerciseslectures :teachers :doc. rndr. daniela walterová, csc.prof. rndr. jitka ulricho

2、vá, csc.rndr. zdenk dvoák, phd.study :continuousdatesubjectno. of less.teacher121.09.2006the scope of biochemistry, its role in medicine.proteins structure, function.3walterová228.09.2006state holiday-305.10.2006heteroproteins.structure and function of hemoglobin. 2walterová412.1

3、0.2006enzymes-structure, classification, mechanism of action. cofactors.3dvoák519.10.2006enzyme activity. regulation of the enzyme activity. enzymes in diagnosis.2walterová626.10.2006nucleotides structure, function.metabolism overview. 2walterová702.11.2006bioenergetics. high-energy c

4、ompounds structure, biological role.biological oxidation overview. 2ulrichová809.11.2006 respiratory chain, oxidative phosphorylation.citric acid cycle. acetylcoa-central role in metabolism. 3ulrichová916.11.2006carbohydrate metabolism over-view. digestion of carbohydrates.2dvoák1023.

5、11.2006glycolysis.gluconeogenesis.2dvoák1130.11.2006glycogen metabolism.pentose phosphate pathway.2dvoák1207.12.2006lipid metabolism overview.digestion of lipids.2walterová1314.12.2005lipoproteins structure, metabolism, diagnostic significance.2walterová1421.12.2006triacylglycero

6、l and phospholipid metabolism.2 walterová1504.01.2007interrelations in carbohydrate and lipid metabolism. 2walterovápractical training:leading teacher :rndr. zdenk dvoák, ph.d.study :continuousweek from-tosubjectno. of less.118.9. - 22.9.2006rules of study and directions for laborator

7、y work. system for blood collection. blood. plasma. serum. 4 (vi)225.9. - 29.9.2006chemical reactions of amino acids and proteins.separation of acidic, neutral and basic amino acids by electrophoresis.determination of isoelectric points of histidine. 4 (va)32.10. - 6.10.2006 thin layer chromatograph

8、y of amino acids. isolation of albumins and globulins by fraction salting out.4 (va)49.10. - 13.10.2006determination of proteins (biuret method).electrophoretic separation of proteins.determination of isoelectric point of casein.4 (va)516.10. - 20.10.2006enzymes introduction: nomenclature, structure

9、, factors influencing enzyme activity.4 (vi)623.10. - 27.10.2006demonstration of enzymatic character of peroxidase reaction.demonstration of substrate specificity of a-amylase and sucrase.inhibition of enzymatic activity - inhibition ofcatalase by cyanide.determination of ph optimum for trypsin and

10、pepsin.4 (zd)730.10. - 3.11.2006chemical reactions of saccharides.polarimetric observations of d-glucose mutarotation. nucleoproteins isolation.4 (dv)86.11. - 10.11.2006chemical reactions of lipids.determination of the iodine number of lipids.4(va)913.11. - 17.11.2006determination of acetylsalicylic

11、 acid in acylpyrin.4 (zd)1020.11. - 24.11.2006deionization of calcium chloride solution on ion exchangers.gel chromatography of hemolyzed blood.4 (dv)1127.11. - 1.12.2006quantitative determination of salicylates by photometry.determination of vitamin c in fruit juices.4 (zd)124.12. - 8.12.2006isolat

12、ion and demonstration of alkaloids fromchelidonium majus.4 (zd)1311.12. - 15.12.2006identification of levomepromazine and its metabolites in biological materials by thin layer chromatography.preparative chromatography of azo dyes on a thin layer.determination of nitrates.4 (zd)1418.12. - 22.12.2006practical photometric test. 4 (dv)151.1. - 5.1.2006chemical properties of substances of biological importance an overview. course unit test.4 (dv)completed by :course unit creditrequir