荧光素激发发射波长

1、荧光素名称激发波长(nm)发射波长(nm)备注1,5 IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine(5-TAMRA)5425685-FAM (5-Carboxyfluorescein)4925185-HAT (H

2、ydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-X-rhodamine)5785676045915-TAMRA(5-Carboxytetramethylrhodamine)5485425525686-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-Aminoactinomycin D (7-AAD)5466477-Hydroxy-4-methylcoumar

3、in360449, 4559-Amino-6-chloro-2-methoxyacridine412, 430471,474ABQ344445Acid Fuchsin540630ACMA(9-Amino-6-chloro-2-methoxyacridine)412, 430471,474Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridine Yellow470550Acrifl

4、avin436520Acriflavin Feulgen SITSA355-425460Aequorin (Photoprotein)466PhotoproteinAFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP,精心整理sgBFPAlexa Fluor 350?346342442441Alexa Fluor 430?431540Alexa Fluor 488?495, 492519, 520Alexa Fluor 532?531,532553, 554Alexa Fluor 546?556, 557572

5、, 573Alexa Fluor 568?577, 578603Alexa Fluor 594?590, 594617, 618Alexa Fluor 633?632650Ale xa Fluor 647?647666Alexa Fluor 660?668698Alexa Fluor 680?679702Alizarin Complexon530-560, 580580, 624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Aminomethylcoumarin)345

6、347425444AMCA-X353442Aminoactinomycin D555655Aminocoumarin346350442445Aminomethylcoumarin (AMCA)345347425444Anilin Blue600Anthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530Ratio Dye, Zn2+APTS424505Astrazon Brilliant Red 4G50058

7、5Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490ATTO- TAG? CBQCA465560ATTO- TAG? FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515精心整理BAO 9 (Bisaminophenyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520Berberine Sulphate43055

8、0Beta Lactamase409447, 520BFP blue shifted GFP (Y66H)BlueFluorescent Protein381,382, 383445, 447, 448blue shifted GFP(Y66H)BlueFluorescent ProteinBFP/GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+Blancophor FFG390470Blancophor

9、 SV370435BOBO? -1462481BOBO? -3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy 505/515502510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-X647665Bodipy 665/67660567

10、6Bodipy Fl504, 505511,513Bodipy FL ATP505514Bodipy Fl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574Bodipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617精心整理Bodipy TR ATP591620Bodipy TR-X SE588616BO- PRO? -1462481BO-PRO ? -3544570Brilliant Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/

11、401533/529Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+459/417517/532Ratio Dye, Zn2+Calcein494517Calcein Blue373440Calcium Crimson?588, 589611,615Calcium Green501,506531Calcium Green-1 Ca2+ Dye506531Ca2+ DyeCalcium Green-2 Ca2+506/503536Ca2+Calcium Green-5N Ca2+506532Ca2+Calcium Green-C18 Ca2+509530Ca2+Calc

12、ium Orange549575576Calcofluor White385, 395, 405437, 440, 445Carboxy-X-rhodamine (5-ROX)576601Cascade Blue?377398399420423Cascade Yellow399400550552Catecholamine410470CCF2 (GeneBlazer)CFDA494520CFP - Cyan Fluorescent Protein430, 433, 436,(453)474, 475, 476,(501)Cyan FluorescentProteinCFP/YFP FRETChl

13、orophyll480650Chromomycin A436-460470Chromomycin A445575CL-NERF (Ratio Dye, pH)504/514540Ratio Dye, pHCMFDA494520Coelenterazine Ca2+ Dye, bioluminescence(429)465Ca2+ Dye, bioluminescence,native moleculeCoelenterazine cp (Ca2+ Dye,)(430)442Ca2+ Dye,bioluminescenceCoelenterazine f(437)473Ca2+ Dye, bio

14、luminescence精心整理Coelenterazine fcp452Ca2+ Dye, bioluminescenceCoelenterazine h(437)464Ca2+ Dye, bioluminescenceCoelenterazine hcp(433)444Ca2+ Dye, bioluminescenceCoelenterazine ip441Ca2+ Dye, bioluminescenceCoelenterazine n(431)467Ca2+ Dye, bioluminescenceCoelenterazine O460575Coumarin Phalloidin387

15、470C-phycocyanineCPM Methylcoumarin384469MethylcoumarinCTC400-450602CTC FormazanCy2?489506Cy3.1 8554568Cy3.5?581598Cy3?514552554566570Cy5.1 8649666Cy5.5?675695Cy5?649666670Cy7?710, 743767, 805Cyan GFP433 (453)475 (501)cyclic AMP Fluorosensor (FiCRhR)500517CyQuant Cell P iferation Assay480520Cell Pro

16、liferation AssayDabcyl453Dansyl340578Dansyl Amine337517Dansyl Cadaverine335518Dansyl Chloride372518Dansyl DHPE336517Dansyl fluoride356noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574精心整理DCFDA504529DCFH (DichlorodihydrofluoresceinDiacetate)505535DDAO463607DHR (Dihydorhodamine 123)505534Di-4-

17、ANEPPS496705Di-8-ANEPPS (non-ratio)488498605713DiA (4-Di-16-ASP)456591Dichlorodihydrofluorescein Diacetate (DCFH)505535DiD - Lipophilic Tracer644665Lipophilic TracerDiD (DilC18(5)644665DIDS341415Dihydorhodamine 123 (DHR)505535DiI (DiIC18(3)549, 551565Dinitrophenol349DiO (DiOC18(3)484, 487501,502DiR7

18、48780Lipophilic TracerDiR (DiIC18(7)750779DM-NERF (high pH)497/510540Ratio Dye, pHDNP349Dopamine340490-520DsRed558583Red fluorescent proteinDTAF494520DY-630-NHS621660Hemicyanelabel forproteins and DNADY-635-NHS634664Hemicyanelabel forproteins and DNAEBFP383447Enhanced BlueFluorescent ProteinECFP4364

19、74Enhanced CyanFluorescent ProteinEGFP488, 498507, 516Enhanced GreenFluorescent ProteinELF 97345530Eosin524545Erythrosin529, 532554, 555Erythrosin ITC529555精心整理Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthD-1)528617Euchrysin430540EukoLightEuropium (III) chlorideEYFP513, 520527, 532Enhan

20、ced YellowFluorescent ProteinFast Blue360440FDA494520Feulgen (Pararosaniline)570625FIF (Formaldehyd InducedFluorescence)405433FITC490, 494520, 525FITC Antibody493517Flazo Orange375-530612Fluo-3480- 506, 506520, 527Fluo-4494516Fluorescein (FITC)490, 494520, 525Fluorescein Diacetate494520Fluoro-Emeral

21、d495524Fluoro-Gold (Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM 1- 43?479598FM 4-46515640Fura Red? (high pH)572657Fura Red?/Fluo-3Fura-2, high calcium335505Excitation ratio dyeFura-2, low calcium363512Excitation ratio dyeFura-2/BCECFGenacryl Brilliant Red B520590Genacryl Brilliant Yello

22、w 10GF430485Genacryl Pink 3G470583Genacryl Yellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type, non-UV excitation475509精心整理(wtGFP)GFP wild type, UV excitation (wtGFP)395509GFPuv385508Gloxalic Acid405460Granular Blue355425Haematoporphyrin530-560580Hoechst 33258

23、345487Hoechst 33342347483Hoechst 34580392440HPTS355465Hydroxycoumarin325-360386-455Hydroxystilbamidine (FluoroGold)361536Hydroxytryptamine400530ndo-1, high calcium330401Emission ratio dyendo-1, low calcium346475Emission ratio dyendodicarbocyanine (DiD)644665ndotricarbocyanine (DiR)748780ntrawhite Cf

24、360430JC-1514529JO-JO-1530545JO-PRO-1532544LaserPro795812Laurodan355460LDS 751 (DNA)543712LDS 751 (RNA)590607Leucophor PAF370430Leucophor SF380465Leucophor WS395465Lissamine Rhodamine572, 577591,592Lissamine Rhodamine B577592LIVE/DEAD Kit AnimalCellsCalcein/Ethidium homodimer494528517617for more det

25、ails refertoLOLO-1566580LO-PRO-1568581Lucifer Yellow425, 428528, 536, 540Lyso Tracker Blue373422Lyso Tracker Blue-White466536精心整理Lyso Tracker Green504, 534511,551Lyso Tracker Red490516Lyso Tracker Yellow551576LysoSensor Blue374424LysoSensor Green442505LysoSensor Yellow/Blue384540Mag Green507531Magda

26、la Red (Phloxin B)524600Mag-Fura Red483/427659/631Ratio Dye, Mg2+Mag-Fura-2369/329369/330508511/491Ratio Dye Ca2+RatioDye Mg2+Mag-Fura-5369/330369/332505/500505/482Ratio Dye, Ca2+RatioDye, Mg2+Mag-lndo-1349/328349/330480/390480/417Ratio Dye, Ca2+RatioDye, Mg2+Magnesium Green506, 507531Magnesium Oran

27、ge550575Malachite Green628Marina Blue362459Maxilon Brilliant Flavin 10 GFF450495Maxilon Brilliant Flavin 8 GFF460495Merocyanin555578Methoxycoumarin360410Mitotracker Green FM490516Mitotracker Orange551576Mitotracker Red578599Mitramycin450470Monobromobimane398490Monobromobimane (mBBr-GSH)398500Monochl

28、orobimane380461MPS (Methyl Green Pyronine Stilbene)364395NBD466539NBD Amine450530Nile Red515-555, 559590, 640Nitrobenzoxadidole465510-650Noradrenaline340490-520Nuclear Fast Red289-530580Nuclear Yellow365495精心整理Nylosan Brilliant lavin E8G460510Oregon Green503522Oregon Green 488-X494517Oregon Green?50

29、3522Oregon Green? 488490, 493514,520Oregon Green? 500497517Oregon Green? 514506526Pacific Blue405455Pararosaniline (Feulgen)570625PBFI340/380420Excitation ratio dyePE-Cy5488670PE-Cy7488755, 767PerCP488675PerCP-Cy5.5488710PE-TexasRed Red 613488613Phloxin B (Magdala Red)524600Phorwite AR360430Phorwite

30、 BKL370430Phorwite Rev380430Phorwite RPA375430Phosphine 3R465565PhotoResist365610Phycoerythrin B PE546-565575Phycoerythrin R PE565578PKH26 (Sigma)551567PKH67496520ChromaPMIA341376Pontochrome Blue Black535-553605POPO-1433457POPO-3533574PO-PRO-1435455PO-PRO-3539567Primuline410550Procion Yellow470600Pr

31、opidium Iodid (PI)(305), 536, 538617PyMPO412, 415561,564, 570Pyrene360387精心整理Pyronine410540Pyronine B540-590560-650Pyrozal Brilliant Flavin 7GF365495QSY 7560Quinacrine Mustard440510Red 613 PE-TexasRed488613Resorufin571584,585RH 414532716Rhod-2552576Rhodamine550573Rhodamine 110496, 497520Rhodamine 12

32、3507529Rhodamine 5 GLD470565Rhodamine 6G525555Rhodamine B540625Rhodamine B 200523-557595Rhodamine B extra550605Rhodamine BB540580Rhodamine BG540572Rhodamine Green502527Rhodamine Phallicidine558, 542575, 565Rhodamine Phalloidine542565Rhodamine Red570590Rhodamine WT530555Rose Bengal525, 540550-600R-ph

33、ycocyanineR-phycoerythrin (PE)565578rsGFP473509red shifted GFP (S65T)S65A471504S65C479507S65L484510S65T488511Sapphire GFP395511SBFI340/380420Excitation ratio dyeSerotonin365520-540Sevron Brilliant Red 2B520595Sevron Brilliant Red 4G500583精心整理Sevron Brilliant Red B530590Sevron Orange440530Sevron Yell

34、ow L430490sgBFP?387450sgBFP? (super glowBFP)387450Quantum ' sSuperGlo?GFPAFPssgGFP?474488sgGFP? (super glow GFP)474509Quantum ' sSuperGlo?GFPAFPsSITS336436Ion ChannelsSITS (Primuline)395-425450SITS (Stilbene Isothiosulphonic Acid)365460SNAFL calcein506/535535/620Ratio Dye, pHSNAFL-1508/54054

35、3/623Ratio Dye, pHSNAFL-2514/543546/630Ratio Dye, pHSNARF calcein552/574590/629Ratio Dye, pHSNARF1576/548635/587Excitation and emission ratio dyeSodium Gree n506, 507532Na+, K+SpectrumAqua433,/53480/55VysisSpectrumGreen497/30, 509/31538/44, 524/56VysisSpectrumOrange559/38, 560588/48VysisSpectrum Red

36、587, 587/35612, 612/51SPQ(6-methoxy-N-(3-sulfopropyl)quinolinium)344443Stilbene335440Sulphorhodamine B can C520595Sulphorhodamine G Extra470570SYTO 11508, 510527, 530Dye for DNA, RNASYTO 12499, 500522, 519Dye for DNA, RNASYTO 13488, 491509, 514Dye for DNA, RNASYTO 14517, 521549, 547Dye for DNA, RNAS

37、YTO 15516, 518546, 555Dye for DNA, RNASYTO 16488, 494518,525Dye for DNA, RNASYTO 17621634Dye for DNASYTO 18490, 493507, 527Dye for DNA, RNASYTO 20512530Dye for DNA精心整理SYTO 21494517Dye for DNASYTO 22515535Dye for DNASYTO 23499520Dye for DNASYTO 24490515Dye for DNASYTO 25521556Dye for DNASYTO 40420441Dye for DNASYTO 41430454Dye for DNASYTO 42433460Dye for DNASYTO 43436467Dye for DNASYTO 44446471Dye for DNASYTO 45452484Dye for DNASYTO 59622645Dye