生物信息学 实验三、核酸序列分析、常用分子信息学软件使用

1、 实验三、核酸序列分析、常用分子信息学软件使用1 、用BioEdit等软件进行序列分析用BioEdit打开FASTA格式的序列Sequence->Nucleic acid-> 1）Sequence->Nucleic acid->Nucleotid compositionDNA molecule: gi|725605238|ref|XM_010330964.1| PREDICTED: Saimiri boliviensis boliviensis interferon, lambda 3 (IFNL3), mRNALength = 744 base pairsMolecu

2、lar Weight = 227718.00 Daltons, single strandedMolecular Weight = 453776.00 Daltons, double strandedG+C content = 61.56%A+T content = 38.44%Nucleotide Number Mol% A 141 18.95 C 251 33.74 G 207 27.82 T 145 19.49 2）Sequence->Nucleic acid->Base composition and mass export(monoisotopic)TitleLENGTH

3、AGCTGapOtherMono_exact_massBaseCompOppositeStrand_exact_massOppositeStrand_BaseCompgi|725605238|ref|XM_010330964.1| PREDICTED: Saimiri boliviensis boliviensis interferon, lambda 3 (IFNL3), mRNA74414120725114500228830.3528025A141 G207 C251 T145230626.6695859A145 G251 C207 T141 3)Sequence->Nucleic

4、acid->Base compoeition and mass export with average massesTitleLENGTHA_COUNTG_COUNTC_COUNTT_COUNTGAP (not used in db)OTHER_COUNTMONO_EXACT_MASSBASE_COMPOPPOSITE_STRAND_MASSOPPOSITE_STRAND_BASE_COMPSEQUENCE (not used in db)OppositeStrand_Ave_massOppositeStrand_BaseCompgi|725605238|ref|XM_010330964

5、.1| PREDICTED: Saimiri boliviensis boliviensis interferon, lambda 3 (IFNL3), mRNA74414120725114500228939.3442020A141 G207 C251 T145230736.4659940A145 G251 C207 T141 4）Sequence->Nucleic acid->complementCACTGTCGGAGTCTCACCCCAGGAAGACGACTGCTTCTGGTCTCTAGTCTTTACTTTGCTCCGTACTCGTCCCCGACGTACCGTCACGACCAC

6、GACTACCGGTGTCACGACTGACAGTGACCTCGTCAAGGACAGTGGTCCGGGGGGTCCCGGGAGGGCCTACGTTCCCCGACGGTGTATCGGGTCAAGTTCAGAGACAGGGGTGTCCTCGACGTTCGGAAATTCTCTCGGTTTCTACGGAATCTTCTCAGCGAAGACGACTTCCTGACGTCCACGGCGAGGGCGGAGAAGGGGTCCTGGACCCTGGACTCCGTCGACGTCCACTCCCTCGCGGGGCACCGGAACCTCCGACTCGACCGGGACTGCGACCTCCAAGACCTCCGGTGGCGACTG

7、TTACTGTACCGGGACCCACTGCAGGACCTGGCCGGGGAAGTGTGGGACGTGGTGCAGGAGAGGGTCGAGGCCCGGACACAGGTCGGAGTCGGGTGCCGTCCCGGGTCCGGGACCCCAGCGGAGGTGGTGACCGACGTGGCCGAGGTCCTCCGGGGTTTTTTCCTCAGGAGACCGACGGACCTCCGGAGACAGTGGAAGTTGGAGAAGGCGGAGGAGTGCGCCCTGGACTTTACGCAGCGGTCGCCCCTGGACACACGGACTGGAAGGGTGGAGGGACGGTGGGTAGTACGTTAGACTCTA

8、AAATAAATATGTAGTTGGTGAACAGAATTAAATAACGGTGGGTTAGCGATA 5）Sequence->Nucleic acid->reverse complementTATCGCTAACCCACCGTTATTTAATTCTGTTCACCAACTACATATTTATTTTAGAGTCTAACGTACTACCCACCGTCCCTCCACCCTTCCAGTCCGTGTGTCCAGGGGCGACCGCTGCGTAAAGTCCAGGGCGCACTCCTCCGCCTTCTCCAACTTCCACTGTCTCCGGAGGTCCGTCGGTCTCCTGAGGAAAAAACC

9、CCGGAGGACCTCGGCCACGTCGGTCACCACCTCCGCTGGGGTCCCGGACCCGGGACGGCACCCGACTCCGACCTGTGTCCGGGCCTCGACCCTCTCCTGCACCACGTCCCACACTTCCCCGGCCAGGTCCTGCAGTGGGTCCCGGTACAGTAACAGTCGCCACCGGAGGTCTTGGAGGTCGCAGTCCCGGTCGAGTCGGAGGTTCCGGTGCCCCGCGAGGGAGTGGACGTCGACGGAGTCCAGGGTCCAGGACCCCTTCTCCGCCCTCGCCGTGGACGTCAGGAAGTCGTCTTCGCTGAG

10、AAGATTCCGTAGAAACCGAGAGAATTTCCGAACGTCGAGGACACCCCTGTCTCTGAACTTGACCCGATACACCGTCGGGGAACGTAGGCCCTCCCGGGACCCCCCGGACCACTGTCCTTGACGAGGTCACTGTCAGTCGTGACACCGGTAGTCGTGGTCGTGACGGTACGTCGGGGACGAGTACGGAGCAAAGTAAAGACTAGAGACCAGAAGCAGTCGTCTTCCTGGGGTGAGACTCCGACAGTG 6）Sequence->Nucleic acid->DNA->RNAGUGACAGCCU

11、CAGAGUGGGGUCCUUCUGCUGACGAAGACCAGAGAUCAGAAAUGAAACGAGGCAUGAGCAGGGGCUGCAUGGCAGUGCUGGUGCUGAUGGCCACAGUGCUGACUGUCACUGGAGCAGUUCCUGUCACCAGGCCCCCCAGGGCCCUCCCGGAUGCAAGGGGCUGCCACAUAGCCCAGUUCAAGUCUCUGUCCCCACAGGAGCUGCAAGCCUUUAAGAGAGCCAAAGAUGCCUUAGAAGAGUCGCUUCUGCUGAAGGACUGCAGGUGCCGCUCCCGCCUCUUCCCCAGGACCUGGGACCUGA

12、GGCAGCUGCAGGUGAGGGAGCGCCCCGUGGCCUUGGAGGCUGAGCUGGCCCUGACGCUGGAGGUUCUGGAGGCCACCGCUGACAAUGACAUGGCCCUGGGUGACGUCCUGGACCGGCCCCUUCACACCCUGCACCACGUCCUCUCCCAGCUCCGGGCCUGUGUCCAGCCUCAGCCCACGGCAGGGCCCAGGCCCUGGGGUCGCCUCCACCACUGGCUGCACCGGCUCCAGGAGGCCCCAAAAAAGGAGUCCUCUGGCUGCCUGGAGGCCUCUGUCACCUUCAACCUCUUCCGCCUCCUCA

13、CGCGGGACCUGAAAUGCGUCGCCAGCGGGGACCUGUGUGCCUGACCUUCCCACCUCCCUGCCACCCAUCAUGCAAUCUGAGAUUUUAUUUAUACAUCAACCACUUGUCUUAAUUUAUUGCCACCCAAUCGCUAU 7)Sequence->Nucleic acid->RNA->DNAGTGACAGCCTCAGAGTGGGGTCCTTCTGCTGACGAAGACCAGAGATCAGAAATGAAACGAGGCATGAGCAGGGGCTGCATGGCAGTGCTGGTGCTGATGGCCACAGTGCTGACTGTCACTGG

14、AGCAGTTCCTGTCACCAGGCCCCCCAGGGCCCTCCCGGATGCAAGGGGCTGCCACATAGCCCAGTTCAAGTCTCTGTCCCCACAGGAGCTGCAAGCCTTTAAGAGAGCCAAAGATGCCTTAGAAGAGTCGCTTCTGCTGAAGGACTGCAGGTGCCGCTCCCGCCTCTTCCCCAGGACCTGGGACCTGAGGCAGCTGCAGGTGAGGGAGCGCCCCGTGGCCTTGGAGGCTGAGCTGGCCCTGACGCTGGAGGTTCTGGAGGCCACCGCTGACAATGACATGGCCCTGGGTGACGTCCTGGA

15、CCGGCCCCTTCACACCCTGCACCACGTCCTCTCCCAGCTCCGGGCCTGTGTCCAGCCTCAGCCCACGGCAGGGCCCAGGCCCTGGGGTCGCCTCCACCACTGGCTGCACCGGCTCCAGGAGGCCCCAAAAAAGGAGTCCTCTGGCTGCCTGGAGGCCTCTGTCACCTTCAACCTCTTCCGCCTCCTCACGCGGGACCTGAAATGCGTCGCCAGCGGGGACCTGTGTGCCTGACCTTCCCACCTCCCTGCCACCCATCATGCAATCTGAGATTTTATTTATACATCAACCACTTGTCTTAA

16、TTTATTGCCACCCAATCGCTAT 8)Sequence->Nucleic acid->TranslateFrame 1:>gi|725605238|ref|XM_010330964.1| PREDICTED: Saimiri boliviensis boliviensis interferon, lambda 3 (IFNL3), mRNA1 GTG ACA GCC TCA GAG TGG GGT CCT TCT GCT GAC GAA GAC CAG AGA 451 Val Thr Ala Ser Glu Trp Gly Pro Ser Ala Asp Glu

17、Asp Gln Arg 1546 TCA GAA ATG AAA CGA GGC ATG AGC AGG GGC TGC ATG GCA GTG CTG 9016 Ser Glu Met Lys Arg Gly Met Ser Arg Gly Cys Met Ala Val Leu 3091 GTG CTG ATG GCC ACA GTG CTG ACT GTC ACT GGA GCA GTT CCT GTC 13531 Val Leu Met Ala Thr Val Leu Thr Val Thr Gly Ala Val Pro Val 45136 ACC AGG CCC CCC AGG G

18、CC CTC CCG GAT GCA AGG GGC TGC CAC ATA 18046 Thr Arg Pro Pro Arg Ala Leu Pro Asp Ala Arg Gly Cys His Ile 60181 GCC CAG TTC AAG TCT CTG TCC CCA CAG GAG CTG CAA GCC TTT AAG 22561 Ala Gln Phe Lys Ser Leu Ser Pro Gln Glu Leu Gln Ala Phe Lys 75226 AGA GCC AAA GAT GCC TTA GAA GAG TCG CTT CTG CTG AAG GAC T

19、GC 27076 Arg Ala Lys Asp Ala Leu Glu Glu Ser Leu Leu Leu Lys Asp Cys 90271 AGG TGC CGC TCC CGC CTC TTC CCC AGG ACC TGG GAC CTG AGG CAG 31591 Arg Cys Arg Ser Arg Leu Phe Pro Arg Thr Trp Asp Leu Arg Gln 105316 CTG CAG GTG AGG GAG CGC CCC GTG GCC TTG GAG GCT GAG CTG GCC 360106 Leu Gln Val Arg Glu Arg P

20、ro Val Ala Leu Glu Ala Glu Leu Ala 120361 CTG ACG CTG GAG GTT CTG GAG GCC ACC GCT GAC AAT GAC ATG GCC 405121 Leu Thr Leu Glu Val Leu Glu Ala Thr Ala Asp Asn Asp Met Ala 135406 CTG GGT GAC GTC CTG GAC CGG CCC CTT CAC ACC CTG CAC CAC GTC 450136 Leu Gly Asp Val Leu Asp Arg Pro Leu His Thr Leu His His V

21、al 150451 CTC TCC CAG CTC CGG GCC TGT GTC CAG CCT CAG CCC ACG GCA GGG 495151 Leu Ser Gln Leu Arg Ala Cys Val Gln Pro Gln Pro Thr Ala Gly 165496 CCC AGG CCC TGG GGT CGC CTC CAC CAC TGG CTG CAC CGG CTC CAG 540166 Pro Arg Pro Trp Gly Arg Leu His His Trp Leu His Arg Leu Gln 180541 GAG GCC CCA AAA AAG GA

22、G TCC TCT GGC TGC CTG GAG GCC TCT GTC 585181 Glu Ala Pro Lys Lys Glu Ser Ser Gly Cys Leu Glu Ala Ser Val 195586 ACC TTC AAC CTC TTC CGC CTC CTC ACG CGG GAC CTG AAA TGC GTC 630196 Thr Phe Asn Leu Phe Arg Leu Leu Thr Arg Asp Leu Lys Cys Val 210631 GCC AGC GGG GAC CTG TGT GCC TGA CCT TCC CAC CTC CCT GC

23、C ACC 675211 Ala Ser Gly Asp Leu Cys Ala End Pro Ser His Leu Pro Ala Thr 225676 CAT CAT GCA ATC TGA GAT TTT ATT TAT ACA TCA ACC ACT TGT CTT 720226 His His Ala Ile End Asp Phe Ile Tyr Thr Ser Thr Thr Cys Leu 240721 AAT TTA TTG CCA CCC AAT CGC TAT 744241 Asn Leu Leu Pro Pro Asn Arg Tyr Each codon is r

24、ead as left nucleotide, top nucleotide, right nucleotideEach entry is organized as follows: The number of occurrences of the codon in the sequence Preference of that codon in organism represented by the codon table (as a fraction of all codons coding for the same amino acid) Three-letter code for th

25、e amino acid coded for according to the codon table |A C G T |- A |4 3 2 1 |A |0.76 0.12 0.04 0.07 | |Lys Thr Arg Ile |- A |1 7 2 1 |C |0.61 0.43 0.27 0.46 | |Asn Thr Ser Ile |- A |4 3 9 5 |G |0.24 0.23 0.03 1 | |Lys Thr Arg Met |- A |3 3 0 1 |T |0.39 0.21 0.13 0.47 | |Asn Thr Ser Ile |- C |1 3 1 0

26、|A |0.31 0.2 0.05 0.03 | |Gln Pro Arg Leu |- C |8 9 6 10 |C |0.48 0.1 0.37 0.1 | |His Pro Arg Leu |- C |9 1 4 20 |G |0.69 0.55 0.08 0.55 | |Gln Pro Arg Leu |- C |2 5 0 3 |T |0.52 0.16 0.42 0.1 | |His Pro Arg Leu |- G |3 5 1 0 |A |0.7 0.22 0.09 0.17 | |Glu Ala Gly Val |- G |10 17 4 7 |C |0.41 0.25 0.

27、4 0.2 | |Asp Ala Gly Val |- G |11 0 2 6 |G |0.3 0.34 0.13 0.34 | |Glu Ala Gly Val |- G |3 3 3 2 |T |0.59 0.19 0.38 0.29 | |Asp Ala Gly Val |- T |0 3 2 2 |A |0.62 0.12 0.3 0.11 | |End Ser End Leu |- T |0 5 6 4 |C |0.47 0.17 0.57 0.49 | |Tyr Ser Cys Phe |- T |0 1 4 2 |G |0.09 0.13 1 0.11 | |End Ser Tr

28、p Leu |- T |2 4 3 2 |T |0.53 0.19 0.43 0.51 | |Tyr Ser Cys Phe |-Frame 2:>gi|725605238|ref|XM_010330964.1| PREDICTED: Saimiri boliviensis boliviensis interferon, lambda 3 (IFNL3), mRNA2 TGA CAG CCT CAG AGT GGG GTC CTT CTG CTG ACG AAG ACC AGA GAT 461 End Gln Pro Gln Ser Gly Val Leu Leu Leu Thr Lys

29、 Thr Arg Asp 1547 CAG AAA TGA AAC GAG GCA TGA GCA GGG GCT GCA TGG CAG TGC TGG 9116 Gln Lys End Asn Glu Ala End Ala Gly Ala Ala Trp Gln Cys Trp 3092 TGC TGA TGG CCA CAG TGC TGA CTG TCA CTG GAG CAG TTC CTG TCA 13631 Cys End Trp Pro Gln Cys End Leu Ser Leu Glu Gln Phe Leu Ser 45137 CCA GGC CCC CCA GGG

30、CCC TCC CGG ATG CAA GGG GCT GCC ACA TAG 18146 Pro Gly Pro Pro Gly Pro Ser Arg Met Gln Gly Ala Ala Thr End 60182 CCC AGT TCA AGT CTC TGT CCC CAC AGG AGC TGC AAG CCT TTA AGA 22661 Pro Ser Ser Ser Leu Cys Pro His Arg Ser Cys Lys Pro Leu Arg 75227 GAG CCA AAG ATG CCT TAG AAG AGT CGC TTC TGC TGA AGG ACT

31、GCA 27176 Glu Pro Lys Met Pro End Lys Ser Arg Phe Cys End Arg Thr Ala 90272 GGT GCC GCT CCC GCC TCT TCC CCA GGA CCT GGG ACC TGA GGC AGC 31691 Gly Ala Ala Pro Ala Ser Ser Pro Gly Pro Gly Thr End Gly Ser 105317 TGC AGG TGA GGG AGC GCC CCG TGG CCT TGG AGG CTG AGC TGG CCC 361106 Cys Arg End Gly Ser Ala

32、Pro Trp Pro Trp Arg Leu Ser Trp Pro 120362 TGA CGC TGG AGG TTC TGG AGG CCA CCG CTG ACA ATG ACA TGG CCC 406121 End Arg Trp Arg Phe Trp Arg Pro Pro Leu Thr Met Thr Trp Pro 135407 TGG GTG ACG TCC TGG ACC GGC CCC TTC ACA CCC TGC ACC ACG TCC 451136 Trp Val Thr Ser Trp Thr Gly Pro Phe Thr Pro Cys Thr Thr

33、Ser 150452 TCT CCC AGC TCC GGG CCT GTG TCC AGC CTC AGC CCA CGG CAG GGC 496151 Ser Pro Ser Ser Gly Pro Val Ser Ser Leu Ser Pro Arg Gln Gly 165497 CCA GGC CCT GGG GTC GCC TCC ACC ACT GGC TGC ACC GGC TCC AGG 541166 Pro Gly Pro Gly Val Ala Ser Thr Thr Gly Cys Thr Gly Ser Arg 180542 AGG CCC CAA AAA AGG A

34、GT CCT CTG GCT GCC TGG AGG CCT CTG TCA 586181 Arg Pro Gln Lys Arg Ser Pro Leu Ala Ala Trp Arg Pro Leu Ser 195587 CCT TCA ACC TCT TCC GCC TCC TCA CGC GGG ACC TGA AAT GCG TCG 631196 Pro Ser Thr Ser Ser Ala Ser Ser Arg Gly Thr End Asn Ala Ser 210632 CCA GCG GGG ACC TGT GTG CCT GAC CTT CCC ACC TCC CTG C

35、CA CCC 676211 Pro Ala Gly Thr Cys Val Pro Asp Leu Pro Thr Ser Leu Pro Pro 225677 ATC ATG CAA TCT GAG ATT TTA TTT ATA CAT CAA CCA CTT GTC TTA 721226 Ile Met Gln Ser Glu Ile Leu Phe Ile His Gln Pro Leu Val Leu 240722 ATT TAT TGC CAC CCA ATC GCT 742241 Ile Tyr Cys His Pro Ile Ala 247Each codon is read

36、as left nucleotide, top nucleotide, right nucleotideEach entry is organized as follows: The number of occurrences of the codon in the sequence Preference of that codon in organism represented by the codon table (as a fraction of all codons coding for the same amino acid) Three-letter code for the am

37、ino acid coded for according to the codon table |A C G T |- A |2 4 2 1 |A |0.76 0.12 0.04 0.07 | |Lys Thr Arg Ile |- A |1 10 7 2 |C |0.61 0.43 0.27 0.46 | |Asn Thr Ser Ile |- A |4 3 10 4 |G |0.24 0.23 0.03 1 | |Lys Thr Arg Met |- A |1 2 5 2 |T |0.39 0.21 0.13 0.47 | |Asn Thr Ser Ile |- C |4 12 0 0 |

38、A |0.31 0.2 0.05 0.03 | |Gln Pro Arg Leu |- C |2 13 3 2 |C |0.48 0.1 0.37 0.1 | |His Pro Arg Leu |- C |7 2 2 10 |G |0.69 0.55 0.08 0.55 | |Gln Pro Arg Leu |- C |1 11 0 3 |T |0.52 0.16 0.42 0.1 | |His Pro Arg Leu |- G |0 4 1 0 |A |0.7 0.22 0.09 0.17 | |Glu Ala Gly Val |- G |1 7 7 3 |C |0.41 0.25 0.4

39、0.2 | |Asp Ala Gly Val |- G |4 2 10 3 |G |0.3 0.34 0.13 0.34 | |Glu Ala Gly Val |- G |1 5 1 0 |T |0.59 0.19 0.38 0.29 | |Asp Ala Gly Val |- T |0 6 10 3 |A |0.62 0.12 0.3 0.11 | |End Ser End Leu |- T |0 11 9 4 |C |0.47 0.17 0.57 0.49 | |Tyr Ser Cys Phe |- T |2 1 12 0 |G |0.09 0.13 1 0.11 | |End Ser T

40、rp Leu |- T |1 4 2 1 |T |0.53 0.19 0.43 0.51 | |Tyr Ser Cys Phe |-Frame3:>gi|725605238|ref|XM_010330964.1| PREDICTED: Saimiri boliviensis boliviensis interferon, lambda 3 (IFNL3), mRNA3 GAC AGC CTC AGA GTG GGG TCC TTC TGC TGA CGA AGA CCA GAG ATC 470 Asp Ser Leu Arg Val Gly Ser Phe Cys End Arg Arg

41、 Pro Glu Ile 1448 AGA AAT GAA ACG AGG CAT GAG CAG GGG CTG CAT GGC AGT GCT GGT 9215 Arg Asn Glu Thr Arg His Glu Gln Gly Leu His Gly Ser Ala Gly 2993 GCT GAT GGC CAC AGT GCT GAC TGT CAC TGG AGC AGT TCC TGT CAC 13730 Ala Asp Gly His Ser Ala Asp Cys His Trp Ser Ser Ser Cys His 44138 CAG GCC CCC CAG GGC

42、CCT CCC GGA TGC AAG GGG CTG CCA CAT AGC 18245 Gln Ala Pro Gln Gly Pro Pro Gly Cys Lys Gly Leu Pro His Ser 59183 CCA GTT CAA GTC TCT GTC CCC ACA GGA GCT GCA AGC CTT TAA GAG 22760 Pro Val Gln Val Ser Val Pro Thr Gly Ala Ala Ser Leu End Glu 74228 AGC CAA AGA TGC CTT AGA AGA GTC GCT TCT GCT GAA GGA CTG

43、CAG 27275 Ser Gln Arg Cys Leu Arg Arg Val Ala Ser Ala Glu Gly Leu Gln 89273 GTG CCG CTC CCG CCT CTT CCC CAG GAC CTG GGA CCT GAG GCA GCT 31790 Val Pro Leu Pro Pro Leu Pro Gln Asp Leu Gly Pro Glu Ala Ala 104318 GCA GGT GAG GGA GCG CCC CGT GGC CTT GGA GGC TGA GCT GGC CCT 362105 Ala Gly Glu Gly Ala Pro

44、Arg Gly Leu Gly Gly End Ala Gly Pro 119363 GAC GCT GGA GGT TCT GGA GGC CAC CGC TGA CAA TGA CAT GGC CCT 407120 Asp Ala Gly Gly Ser Gly Gly His Arg End Gln End His Gly Pro 134408 GGG TGA CGT CCT GGA CCG GCC CCT TCA CAC CCT GCA CCA CGT CCT 452135 Gly End Arg Pro Gly Pro Ala Pro Ser His Pro Ala Pro Arg

45、Pro 149453 CTC CCA GCT CCG GGC CTG TGT CCA GCC TCA GCC CAC GGC AGG GCC 497150 Leu Pro Ala Pro Gly Leu Cys Pro Ala Ser Ala His Gly Arg Ala 164498 CAG GCC CTG GGG TCG CCT CCA CCA CTG GCT GCA CCG GCT CCA GGA 542165 Gln Ala Leu Gly Ser Pro Pro Pro Leu Ala Ala Pro Ala Pro Gly 179543 GGC CCC AAA AAA GGA G

46、TC CTC TGG CTG CCT GGA GGC CTC TGT CAC 587180 Gly Pro Lys Lys Gly Val Leu Trp Leu Pro Gly Gly Leu Cys His 194588 CTT CAA CCT CTT CCG CCT CCT CAC GCG GGA CCT GAA ATG CGT CGC 632195 Leu Gln Pro Leu Pro Pro Pro His Ala Gly Pro Glu Met Arg Arg 209633 CAG CGG GGA CCT GTG TGC CTG ACC TTC CCA CCT CCC TGC CAC CCA 677210 Gln Arg Gly Pro Val Cys Leu Thr Phe Pro Pro Pro Cys His Pro 224678 TCA TGC AAT CTG AGA TTT TAT TTA TAC ATC AAC CAC TTG TCT TAA 722225 Ser Cys Asn Leu Arg