试验中动物安乐死时机及准则

1、IACUC参考资料中勤物安架死日寺械及？ww背景 Background中勤物安架死畤械及型JW由IACUC制言丁，主要在祝靶尚未完成之勤物彝生所引 起或非55期症状，而必须符勤物安架死之状况。These criteria have been established by the Institutional Animal Care and Use Committee, which recognizes that euthanasiais sometimes necessaryprior to the scheduled end of a study, either because of unan

2、ticipated complications, or because of the protocol itself.型即 Criteria此型JW逾用於所有中或未之勤物。除了IACUC已寄查同意之情形(如：引起之 58期中症状且能使勤物痛苦程度减至最低)，否JW只要符合下列任一情况即需符勤物安架 死。These criteria apply to all animals on experimental protocols, whether manipulated or not. Fulf川ment of one criterion can constitute grounds for eut

3、hanasia. Exceptions are permitted only if approved by the Committee as part of the protocol review process (i.e. the clinical signs listed below are expected as part of the experiment and appropriate measures are taken to minimize pain or discomfort in the animals).1 . It重MS Weight loss:醴重减20-25%,或是

4、勤物出琪悲病II或消耗性症候畤。*非生辰期勤物醴重减可依a®&物刚迤勤物房之醴重或平均年龄醴重悬依at。生辰期之勤物醴重或言午不曾下降，但若瓢法正常增重，仍鹰判悬醴重减Weight loss of 20-25% (depending on attitude, weight recorded at time of arrival, and age: growing animals may not lose weight, but may not gain normally) or if not measured, characterized by cachexia and wa

5、sting syndrome.2 .丧失食愁 Inappetance:小型物完全丧失食愁连24小日寺或食愆不佳（低於正常量之50%）连”畤 大勤物完全丧失食愁连5天或食悠不佳（低於正常量之50%）连以畤。Complete anorexia for 24 hours in small rodents, up to 5 days in large animals; partial anorexia (less than 50% of caloric requirement) for 3 days in small rodents, 7 days in large animals.3 .虚弱（瓢法i

6、fi食或欧水） Weakness/inability to obtain feed or water:勤物在没有麻醉或的状魅下，24小日寺瓢法站立或趣度勉弓金才可站立日寺Inability or extreme reluctance to stand, that persists for 24 hours, assuming that the animal has recovered from anesthesia.4 .垂死/K死 Moribund state:勤物在没有麻醉或WT的状魅下，表琪精神抑修伴随H温谩低(低於37C)畤。Depression coupled with body t

7、emperature below 37 ,5 .感染 Infection:瓢谪是明18可知或因H温升高白血球数目增加而判断悬感染所致，且在抗生素治瘵瓢效或伴随勤物全身性不逾症状出？I畤。Infection involving any organ system (either overt, or indicated by increased body temperature or WBC parameters), which fails to respond to antibiotic therapy within an appropriate period of time and is acc

8、ompanied by systemic signs of illness.6 .出垣器官殿重丧失功能的陶床症状且治瘵瓢效，或勤物中心Sttt肺判断58彳爰不佳畤。如：Signs of severe organ system dysfunction and non-responsive to treatment, or with a poor prognosis as determined by an veterinarian:呼吸系统：呼吸困!隹、Respiratory system: dyspnea, cyanosis.心血管系统：大失血、已给予一次输液治瘵彳爰仍贫血(低於 20%)Car

9、diovascular system: blood loss or anemia.(3)消化系统：殿重幅吐或下痢，消化道阻塞，套叠，腹膜炎，内麒摘除手衍Gastrointestinal system: severe vomiting or diarrhea, obstruction, intussuception; peritonitis, evisceration(immediate euthanasia required).(4)泌尿道系统：胃衰竭(BUN, creatinine, uropehtoneum的提升)Urogenital system: renal failure chara

10、cterized by elevated BUN, creatinine or uroperitoneum.(5)神系统：中福神抑制、震II、IS痪(其中任一肢或以上)、封止痛剜治瘵瓢效之 疼痛Nervous system: CNS depression, seizures, paralysis of one or more extremities; pain unresponsive to analgesic therapy.(6)肌肉骨骼系统：肌肉受揖或骨折使肢H丧失功能期彝生亚通谩IACUC寄核除外)Musculoskeletal system: muscle damage or fra

11、cture resulting in inability to use the limb, unless anticipated as part of the study.(7)皮唐：瓢法治之像口、重褪性自残或二级以上之保温塾煲像Integumentary system: Non-healing wounds, repeated self-trauma, second or third degree heating pad burns.7 .月重瘤生成黑屋平估 Tumorigenesis Endpoints Assessment瓢谪自彝性或是接槿的月重瘤，均鹰迤行黑屋平估。常勤物身上樊琪月重瘤

12、，每遇 至少检查雨次月重瘤生房情形，雨次检查的IW隔不可超谩四大。只要符合下列任一情 况即需符勤物安架死。Tumors may occur spontaneously, or be experimentally induced through transplantation or administration of carcinogens. Animals in which tumors are growing should be checked at least two times weekly, at intervals no greater than four days apart. T

13、he clinical signs listed below are expected as part of the experiment and appropriate measures are taken to minimize pain or discomfort in the animals.1)覃一月重瘤的重量超谩勤物醴重的10%,或是成年小鼠月重瘤平均直彳至超谩 20mm,或是 成年大鼠月重瘤平均直彳至超谩40mm。A tumor burden greater than 10% body weight, and in an adult mouse, a mean tumor dia

14、meter exceeding 20 mm or in an adult rat, a mean tumor diameter exceeding 40 mm.2) 11表月重瘤：月重瘤表面出？I溃瘪、壤死或是感染。Superficial tumors： Tumors that ulcerate, become necrotic or infected.3)腹腔月重瘤：腹腔累常呼吸困!|隹。Ascitic tumors - abdominal distension, dyspnea.4)内月重瘤：神症状。Intracranial tumors - neurological signs虱物安槃死

15、方法 Method of euthanasia of animals at the end of study表1.嚼衡勤物安架死方法方法，展孕13 日胚胎01-06日龄07-14日龄<200g>200g二氧化碳00VVV1 .使用高屋桶装二氧化碳2 .孰行日寺，儒可能在遽雕勤物嗣餐房的非公 埸所迤行3 . 14日龄以下勤物，不建褐使用二氧化 碳安架死，需配合SfHHalothane Methoxyflurane Isoflurane Enflurane00VVV1 . 须具彳着汽化器等事用彳着，言十合遹的 可透视性密朗容器以易於觐察勤物2 .大部分的吸入性!剜封人ft有害，需在通

16、凰良好埸所轨行SevofluraneDesflurane3 .麻醉期勤物可能出现挣扎及焦Zt4 . 7公斤以上不建tg使用此方法。Pentobarbital 100-150mg/kg.IP, IV00VVV1 . JS於第三级管制蕖品，人iOS先向南生署 申日青使用官午可2 .人有保定勤物及注射的技秫亍麻醉彳爰放血000VV1 .放血不完整或心IW采血不完整日寺，勤物可 能曾甦醒2 .人有相信司技秫亍麻醉彳爰SfHVVVVV1 .人有相信司技秫亍2 .仔鼠需以利的外科剪刀其他即使 用台低温麻醉彳爰断VV000符仔鼠放置檄本杯（非petri dish ）,浸入冰it中及勺二十分金童，以减少月管部

17、活性及流血麻醉彳爰5（椎脱 臼000Vg人有相信司技秫亍麻醉彳爰注射 KCl.2meq/kg, IV000VV人有青靴辰注射的技街清醒中勤物断VVddd1 .人有相信司技秫亍2 .仔鼠需以利的外科剪刀其他使用 断理（台清醒中勤物监直 椎脱臼000dd人有相信司技秫亍乙醴003dd乙醴具有易燃、易爆的性11,封呼吸到黏膜 刺激性弓肌 如因瞬需求而使用乙醴，即需 全程在抽氧槽内操作，勤物ftut需置於防爆 冰檀内V:推U使用的方法。0:不推U使用的方法。8：系墨械横内勤物管理委H曾同意彳爰使用 的方法。ip：腹腔注射。IV： IM辰注射。表.2 100% CO2安架死畤IW参考襟举Age of m

18、iceCO2 exposure TimeRemark0-6 days60 mins一般需配合断法合伊使用7-13 days20 mins一般需配合断壁_1法合伊使用14-20 days10 mins叁 21 days5 mins来源：Comp Med 55(3), 275-281, 20051. 阈防ft孥院勤物中言青表附件H:中勤物安架死畤械及型刖2. AVMA Guidelines on Euthanasia.2007.American Veterinary medical Association.3. Euthanasia of Reptiles in New Zealand: Curr

19、ent Issues and Methods. B.D.(Brett) Gartrell, E.J.(Ted) Kirk. kokako (2005) 12(1), 12-15.4. Guidelines for tumor inoculation in rodents.( /animals/downloads/ SOPs/Tumor_Endpoints_Guidelines.pd)f5. Http://ARAC/endpoints.pdf. Guidelines for endpoints in animal study proposals.