神经胶质细胞文献回报ppt课件

1、Evidence for NG2-glia Derived, Evidence for NG2-glia Derived, Adult-Born FunctionalAdult-Born FunctionalNeurons in the HypothalamusNeurons in the HypothalamusSarah C. Robins1, Eric Trudel2, Olivia Rotondi1, Xiaohong Liu1, Tina Djogo1, Darya Kryzskaya1,Charles W. Bourque2, Maia V. Kokoeva1*汇报人：学号：;思绪

2、： 有文献报道，鼠科动物下丘脑有生成神经元的功能，但是是哪种细胞具有这种功能？进展鉴定 脑室膜细胞ventricular tanycytes被鉴定为是神经元生成的来源，但是研讨发现，由此产生的神经元占总新神经元的很少部分，另一个来源是？ 有报道下丘脑细胞能产生具有神经元标志物NeuN or HuC/D (Hu)的细胞,但是缺乏电生理的证明? Rivers et al用基因命运跟踪技术证明表达PDGFRa的细胞能在成年鼠的梨状皮层中产生投射神经元(但是其他人用Olig2-CreER or PDGFRa-CreER mice不能证明此结果或提出质疑), 那么NG2-glia是不是也可以产生neur

3、on？; 这篇文章主要是运用转基因技术,失踪技术,对下丘脑中NG2-glia进展不同时期的察看,并对最终分化而成的的带有神经元marker的细胞进展电生理鉴定,最终阐明NG2-glia是成体下丘脑中新生神经元的主要来源.;NG2-glia:NG2-glia是以硫酸软骨素蛋白聚醣(NG2)和血小板衍生物生长因子受体alpha(PDGFRa)为标志物的神经胶质细胞;占成体中枢神经系统细胞的10%;可以分裂增殖,产生少突胶质细胞;有文章报道,其在梨状皮层(piriform cortex, 和下丘脑一样都属于边缘系统)中能产生少量神经元,但是也有文章对此质疑;A为脑室灌注一次24h后,下丘脑实体成对出

4、现BrdU+的细胞,阐明由迁徙而来的能够性不大;BC可以看出分裂细胞细胞广泛分布于下丘脑,没有富集景象;BD是七天灌注后立刻取样察看和28天后察看的对比无明显分布，阐明这些细胞的不是迁徙来的；E显示BrdU+ cell isNG2-glia下丘脑中存在大量增值细胞，而非迁移过来的，大部分是NG2-glia;有报道，Sox2+ cell能分化为神经元，那么NG2-glia假设能分化为神经元，也能够产生Sox2A:Sox2+ cells主要分布在第三脑室附近和下丘脑实体；CDE:三种双阳性类型；ABF：37%+2.8%NG2+、Sox2+双标志，呈均匀分布，NG2是主要的增值细胞; Co-labe

5、lling with NG2 and Sox2 antibodies indicated that 37.0% +2.8 of hypothalamic NG2-glia robustly express Sox2 (Fig. 2A,B,F). Despite the fact that NG2-glia accounted for fewer than 10% of the total Sox2+ cell population, almost all (92.2%+2.8) of the hypothalamic BrdU+/Sox2+ cells also expressed NG2 (

6、Fig. 2A,B,F), suggesting that NG2-glia are the only substantially proliferative subpopulation of Sox2+ cells in the adult hypothalamic parenchyma. three types of NG2-glia pairs according to Sox2 expression: i) substantial Sox2 expression in both cells; ii) no to barely detectable Sox2 expression in

7、both cells; or iii) Sox2 expression limited to one cell (Fig. 2CE), relate to differences in neural fates.;BC：NG2是下丘脑主要的增值细胞；D-F：30days postTX 还有NG2细胞表达；L：7天和60天后，双阳性的绝度数量一样，2-7天少突胶质细胞迅速增多为重组得的保真性提供根底；GH：外表不表达星形胶质细胞的marker，即不分化为星形胶质细胞；LM：Tom+细胞中：NG2+数量和比率减少，而APC+相反; More than two thirds of the detec

8、ted BrdU+/EdU+ cells expressed NG2 (Fig. 3B,C), indicating that adult hypothalamic NG2-glia can undergo multiple self-renewing divisions, in line with findings for the cerebral cortex 11； one of the primary functions of adult hypothalamic NG2-glia is to provide a continuous supply of new oligodendro

9、cytes；;Figure S1 Fidelity of the NG2creER:tdTomato reporter. Mice were treated with TX twice daily for two days. (A) Cell types were determined by colocalisation of Tom with antibodies to NG2 to identify NG2-glia (arrowhead N), CD13 to identify pericytes (arrowhead P) and APC to identify oligodendro

10、cytes (arrowhead O). (B) Quantification of results from A. Labelling with antibodies to HuC/D (C) and NeuN (D) confirmed that Tom+ neurons were not present at this timepoint.重组实验的保真性研讨：周皮细胞和已分化神经细胞不表达tom;A-C：tom+和HuC/D+细胞被检出；C-D：a-tom+和NeuN+被检出；G：双标志细胞随时间呈线性增长；H：tom+中Hu+和Neu+的比率不同，代表的时期不同，不同的偏好等缘由;A：第一次注射TX，后继续饮用含有BrdU的水直到，处死取样，发现Hu+/Tom+/BruU+的三标细胞；B： Hu+/Tom+中BruU+的比率和tom+中BruU+的比率没有明显不同这组实验阐明NG2-glia分化为了神经