限制性酶的酶切位点

1、各种限制性酶的酶切位点Enzyme NameAatlAatllAccIAccIIAccIIIAcylAfllAflllAflUIAhalAhallAhalIIAlulAlwlAlwlAlwNIAocIAocIIAos IAos 11SequenceAGG!CCTGACGT!CGT!MKACCG!CGT!CCGGAGR!CGYCG!GWCCC!TTAAGAiCRYGTCC!SGGGR!CGYCTTT!AAAAG! CT GGATCNNNN! iNNNNNGATCC CAGNNN!CTG CC!TNAGGGDGCH!C TGC!GCAGR!CGYCApalGGGCC!CApaLIGiTGCACAp

2、ylCC!WGGAquIC!YCGRGAselAT!TAATAspIGAANN!NNTTCAspIG!GTACCAspIGACN!NNGTCAsulG!GNCCAsuIITT!CGAAAvalC!YCGRGAvailG!GWCCAvalIIATGCAiTAvrl!CYCGRGAvrilC!CTAGGAxylCC!TNAGGBallTGG!CCABamHIGiGATCCBanIGiGYRCCBanllGRGCY!CBanlllAT!CGATBbelGGCGC!CBbill/AcylGR!CGYCBbvlGCAGCNNNNNNNN!Bbvl!NNNNNNNNNNNNGCTGCBbvl IGAAGA

3、CNN!Bbvl I!NNNNNNGTCTTCBceflACGGCNNNNNNNNNNNX!Bcefl!NXNNNN1WNNNNGCCGTBellT!GATCABeniCC!SGGBgHGCCNNNNJNGGCBglllAiGATCTBini!NNNNNGATCCBiniGGATCNNNN!BsePI!GCGCGCBsmAI!GTCTCBsmAI!GAGACBsmIGAATGCN!BsmIGiCATTCBspI GDGCH!CBspHIT!CATGABspMIACCTGCNNNN!BspMI!NNNNNNNNGCAGGTBspMIIT!CCGGABsrlACTGGN!BsrlC!CAGTBss

4、HIIGJCGCGCBstBITT!CGAABstEIIG!GTNACCBstIGiGATCCBstNICC!WGGBstPIGiGTNACCBstUICG!CGBstXICCANNNNN!NTGGBstYIR!GATCYBsulCC!TXAGGCcrIC!TCGAGCfolGCG!CCfrIR! CCGGYCfrIG!GNCCCfrIY!GGCCRClalAT!CGATCviJIRG!CYCvnICC!TNAGGDdelC!TNAGDpnlGA! TCDralTTT!AAADrallRG!GNCCYDralllCACNNN!GTGDsalC!CRYGGEaelY!GGCCREagIC!GGC

5、CGEarl!CTCTTCEarl!GAAGAGEclXIC!GGCCGEcolTAC!GTAEcolGGTCTCN!Ecol!NNNNNGAGACCEcolG!GWCCEcoIIIAGC!GCTEcolC!GGCCGEcol!CTGAAGEcol!CTTCAGEcolCC!TXAGGEcoNICCTNNJNNNAGGEcoOIRG!GNCCYEcoRIGiAATTCEcoRII!CCWGGEcoRVGAT!ATCEcoTIC! CWGGEcoTIATGCA!TEcoTIGRGCY!CEhelGGC!GCCEspIGC!TNAGCFinI!GTCCCFinI!GGGACFnuHIGC!NGCF

6、okIGGATGNNNNNNNNN!FokI!NXNNNNNNNXNNNCATCCFspITGC!GCAGdilliNXNNNYGGCCGGdillCGGCCRN!Gsul!CTCCAGGsul(CTGGAGHaelWGG!CCWHaellRGCGC!YHaelllGG!CCHap 11C!CGGHgalGACGCNNNNN!Hgal!NNNNNNNNNXGCGTCHgiAIGWGCWiCHgiEIIiACCNNNNNNGGTHhalGCG!CHinIGRiCGYCHinPIG!CGCHindiGTY!RACHindHIA!AGCTTHinflG!ANTCHpalGTT!AACHpallC!C

7、GGHphIGGTGANNNNNNNN!HphI!NNNNNNNTCACCKpnlGGTAC!CKspICTCTTCN!KspI!NNNNGAAGAGMae IC!TAGMae 11A!CGTMaelll!GTNACMb。I!GATCNfboIIGAAGANNNNNNNN!MboII!NNNNNNNTCTTCMfel!CAATTGMfllR!GATCYMluIAJCGCGTMme I!TCCRACMme I!GTYGGAMnllCCTCNNNNNNN!MnlliNXNNNNNGAGGMrolT!CCGGAMselT!TAAMspIC!CGGMstITGC!GCAMstllCC!TNAGGMva

8、lCC!WGGNaelGCC!GGCNariGG!CGCCNeilCC!SGGNcolC!CATGGNdelCA!TATGNdell!GATCNhelG!CTAGCNlalllCATG!NlalVGGXiNCCNot IGC!GGCCGCNrulTCG!CGANsilATGCA!TNspOlRCATGJYNsp () VTT!CGAANspBIICMG!CKGNspIIGDGCH!CNspIIIC!YCGRGNspIVG!GNCCNun 11GG!CGCCPaeRC!TCGAGPallGG!CCPflMICCANNNNiNTGGPielGAGTCNNNN!Piel!NNNNNGACTCPmaC

9、ICAC!GTGPpuMIRG!GWCCYPstICTGCA!GPvulCGAT!CGPvuIICAG!CTGRsalGT! ACRsrlGiAATTCRsrllCG!GWCCGSadGAGCT!CSacIICCGC!GGSallG!TCGACSauAI!GATCSaulG!GNCCSaulCC!TNAGGSealAGT!ACTScrFICC!NGGSdulGDGCH!CSeelC!CNNGGSexi!CTCGAGSfaNIGCATCNNNNN!SfaNI1NNNNNNNNNGATGCSfilGGCCNNNN!NGGCCSinIG!GWCCSmalCCC!GGGSnaBITAC!GTASnal!GTATACSpelA!CTAGTSphIGCATGiCSplIC!GTACGSspIAAT!ATTSstIGAGCT!CSstHCCGC!GGSstHI!ACGTStulAGG!CCTStylC! CWGGStySJI!GAGNNNNNNGTRCStySJI!GYACNNNNNXCTCTaqIT!CGATaqIIGACCGANNNNNNNNNNX!TaqIIiNNNNNNNNNTCGGTCTaqIICACCCANNNNXNNNNNX!TaqII1NNNNNNNNNTGGGTGThalCG!CGTspI!GTSACTspEI!AATTTthIGACNiNNGTCTthl