经皮植入记忆合金自体膨胀支架体外研究课件

1、2022-4-7经皮植入记忆合金自体膨胀支架体外研究Implantation of Endothelial Progenitor Cells on a Self-expanding Metal Stent: an in vitro StudyHongjian Shi,MDGaojun Teng,Ph.D,MDAihong Cao,MDDeparment of Radiology, Zhongda Hospital, Southeast Universty2022-4-7经皮植入记忆合金自体膨胀支架体外研究ContentoBackgroundoMaterials and methodsoResu

2、ltsoDiscussion2022-4-7经皮植入记忆合金自体膨胀支架体外研究BackgroundoRestenosis:pseudointimal hyperplasia ( PTA, stent)oHigh restenosis in TIPS(30%-50%, 1y)oBile leakage unavoidable during TIPSoBile inhibit both SMC and ECoCovered Viattor stent 1. Coldwell D, Radiology 1995 2. Zhuang Z,AJR 2002 3. LaBerge J,JVIR 1993

3、 4. Nishimine K,Radiology 1995 5. Teng G,Radiology 1998;2005 6. Cejna M,Radiology 2001 7. Hausegger K,JVIR 20042022-4-7经皮植入记忆合金自体膨胀支架体外研究Background oEPC(endothelial progenitor cell) can differentiate into EC(endothelial cell) and incorporate into sites of active angiogenesis. 2022-4-7经皮植入记忆合金自体膨胀支架体

4、外研究Shantsila E, et al.JACC 20072022-4-7经皮植入记忆合金自体膨胀支架体外研究2022-4-7经皮植入记忆合金自体膨胀支架体外研究EPC Isolation,Culture,Identification2022-4-7经皮植入记忆合金自体膨胀支架体外研究Representative relative distribution of some of the antigens expressed inCECs, as depicted by flow cytometry. Bertolini F et al. Drug Discovery Today 2007;

5、12:806-8122022-4-7经皮植入记忆合金自体膨胀支架体外研究Photographs of EPC2022-4-7经皮植入记忆合金自体膨胀支架体外研究EPC uptaken DiI-Ac-LDLEPC binding FITC-UEA-12022-4-7经皮植入记忆合金自体膨胀支架体外研究Immunocytochemistry EPC cell markers CD34, KDR,vWF2022-4-7经皮植入记忆合金自体膨胀支架体外研究Fibrinogen Preparationo15 ml peripheral blood from the same animaloCryopre

6、cipitated technique Centrifugation at 1250 rpm at 4 for 20 min the plasma frozen to 70, stored at -20 4 refrigerator centrifugation at 2500 rpms at 2 for 40 min o1.5ml concentrated fibriongen DePalma L, Transfusion 19932022-4-7经皮植入记忆合金自体膨胀支架体外研究Procedure of EPC seeding on stento10 self-expanding wov

7、en metal stents(Microtech, Nanjing): 5 in case group and 5 in control group.oFibrin gel(fibrinogen 5mg/ml+thrombin 0.1U/ml)oIncubate for 24 h (rotate every 8 h) in EGM-2 with EPC concentration 1106 /mloCell count oRinsed EPC re-identified 1 Kipshidze N, JACC 2000 2 Shirota T, Biomaterials 20032022-4

8、-7经皮植入记忆合金自体膨胀支架体外研究2022-4-7经皮植入记忆合金自体膨胀支架体外研究Cell countoRinsed cell from EPC seeded stent: 21052022-4-7经皮植入记忆合金自体膨胀支架体外研究DiscussionoIt is feasible to devise an EPC-seeded stent in vitrooApplicabe in TIPS as well as vascular systemoEPC-seeded stent with photocured-geloCD34 antibody capture EPC Stent 1 Shirota T, Biomaterials 2003 2 Aoki J, JACC 20052022-4-7经皮植入记忆合金自体膨胀支架体外研究AcknowlegementoThis study was supported by grant 30670604 of the national natural science foundation of ChinaoStents were sponsored by( Microtech Co.,Nanjing)oThanks for m