MiRpCUEDC轴功能失调导致巨噬细胞功能障碍和结肠癌

1、MiRpCUEDC轴功能轴功能失调导致巨噬细胞失调导致巨噬细胞功能障碍和结肠癌功能障碍和结肠癌军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical Sciences研究背景研究背景研究思路研究思路研究结果研究结果军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical Sciences常用名词 CUEDC2：CUE构造域蛋白2 tumor-associated macrophages (TAMs)：肿

2、瘤相关性巨噬细胞 Colorectal cancer (CRC) ：结直肠癌 ko mice 基因敲除小鼠 RNA-induced silencing complexRISE:RNA诱导沉默复合体军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical Sciences研究背景研究背景 Colorectal cancer (CRC) is the third most common malignancy 结直肠癌是排行第三的恶性肿瘤军军事事医医学学科科学学院院附附属属医医院院Affiliated H

3、ospital of the Academy of Military Medical Sciences研究背景研究背景It is known that intestinal inflammation is one of the major causes leading to CRC Inflammatory bowel diseases (IBDs) are inflammatory disorders of the intestinal tract, which often result in the development of colitis-associated cancer(CAC)

4、。肠道炎症是引起炎症性肠病的主要原因，由此导致结肠炎相关性肿瘤军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical Sciences研究背景研究背景 Macrophages are the most prominent innate immune cells that play a central role in host defense and inflammation. 巨噬细胞是最重要的固有免疫细胞，在宿主抗炎中起了核心作用军军事事医医学学科科学学院院附附属属医医院院Affiliated

5、Hospital of the Academy of Military Medical Sciences研究背景研究背景CUE构造域蛋白是能够调控炎症的蛋白，但是否在影响肿瘤生长方面知道甚少像很多实体瘤，结肠癌内很有多免疫细胞浸润，包括巨噬细胞。而巨噬细胞是最著名的先天性免疫细胞，在宿主防御和炎症中起核心作用军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical Sciences研究思路CUEDC2CUEDC2与巨噬细胞的关系与巨噬细胞的关系CUEDC2CUEDC2与炎性因子的关系与炎性因子的关系

6、MiR-324-5pMiR-324-5p与与CUEDC2CUEDC2的关系的关系CUEDC2CUEDC2与肿瘤的关系与肿瘤的关系 动物实验 人体标本检测军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical Sciences研究结果单核细胞向巨噬细胞分化过程中CUEDC2上调军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical Sciencesu CuedcCuedc基因敲除小鼠炎性细胞因子上调基因

7、敲除小鼠炎性细胞因子上调研究结果军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical Sciences研究结果CUEDC2在人体巨噬细胞调节细胞因子中起了同样重要的作用军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical Sciences研究结果 MiR-324-5p Regulates the Differential Expression ofCUEDC2 in Monocytes and M

8、acrophages军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical SciencesCUEDC2 KO小鼠对肠炎高度易感研究结果军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical Sciences人结肠癌巨噬细胞中的CUEDC2表达显著下降研究结果军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical Sciences人体内CUEDC减少导致IL-4及miR-324-5p的高表达研究结果军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical SciencesCUEDC2缺失促进结直肠癌的开展研究结果军军事事医医学学科科学学院院附附属属医医院院Affiliated Hospital of the Academy of Military Medical Sciences总结我们的研究结果提示miR-324-5p在调节巨噬细