重组人瘦素作用机制 - Medchemexpress - MCE中国

1、Product Data SheetRecombinant Human LeptinCat. No.: HY-P7232Synonyms: rHuLeptin; Obesity protein; OBSpecies: HumanSource: E. coliAccession: P41159Gene ID: 3952Molecular Weight: Approximately 16.0 kDaPROPERTIESAA SequenceV P I Q K V Q D D T K T L I K T I V T R I N D I S H T Q S V S S K Q K V T G L D

2、F I P G L H P I L T L S K MD Q T L A V Y Q Q I L T S M P S R N V I Q I S N D L E N L R D L L H V L A F S K S C H L P W A S G L E T L D S L G G V L E A S G Y S T E V V A L S R L Q G S L Q DM L WQ L D L S P G CBiological Activity The ED50 is 5.0 105 units/mg.Appearance Lyophilized powder.Formulation L

3、yophilized after extensive dialysis against PBS, pH 7.4.Endotoxin Level 1 EU/g, determined by LAL method.Reconsititution Reconstitute the lyophilized recombinant Human Leptin (rHuLeptin) to 0.1-1.0 mg/mL using sterile distilled water oraqueous buffer containing 0.1% BSA.Storage & Stability Lyophiliz

4、ed recombinant Human Leptin (rHuLeptin) is stored at -20C. After reconstitution, it is stable at 4C for 1week or -20C for longer. It is recommended to freeze aliquots at -20C or -80C for extended storage.Shipping Room temperature in continental US; may vary elsewhere.DESCRIPTIONBackground A sensor (

5、leptin production by adipose cells) monitors the size of the adipose tissue mass. Hypothalamic centersreceive and integrate the intensity of the leptin signal through leptin receptors (LRb). Effector systems, including thesympathetic nervous system, control the two main determinants of energy balanc

6、e-energy intake and energyexpenditure1. Recessive mutations in the leptingene are associated with massive obesity in mice and humans,establishing a genetic basis for obesity. Leptin circulates in blood and acts on the brain to regulate food intake andPage 1 of 2 www.MedChemEenergy expenditure. When

7、fat mass falls, plasma leptin levels fall, stimulating appetite and suppressing energyexpenditure until fat mass is restored. When fat mass increases, leptin levels increase, suppressing appetite untilweight is lost. This system maintains homeostatic control of adipose tissue mass2.REFERENCES1. A se

8、nsor (leptin production by adipose cells) monitors the size of the adipose tissue mass. Hypothalamic centers receive and integrate the intensity ofthe leptin signal through leptin receptors (LRb). Effector systems, including the sympathetic nervous system, control the two main determinants of energy

9、balance-energy intake and energy expenditure1. Recessive mutations in the leptingene are associated with massive obesity in mice and humans,establishing a genetic basis for obesity. Leptin circulates in blood and acts on the brain to regulate food intake and energy expenditure. When fat mass falls,p

10、lasma leptin levels fall, stimulating appetite and suppressing energy expenditure until fat mass is restored. When fat mass increases, leptin levels increase,suppressing appetite until weight is lost. This system maintains homeostatic control of adipose tissue mass2.2. Friedman JM, et al. Leptin and the regulation of body weigh. Keio J Med. 2011;60(1):1-9.McePdfHeightCaution: Product has not been fully validated for medical