4-Methylumbelliferone-Hymecromone-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemE4-MethylumbelliferoneCat. No.: HY-N0187CAS No.: 90-33-5Synonyms: Hymecromone; 4-MU分式: CHO分量: 176.17作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 300 mg/mL (1702.90 m

2、M)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 5.6763 mL 28.3817 mL 56.7634 mL5 mM 1.1353 mL 5.6763 mL 11.3527 mL10 mM 0.5676 mL 2.8382 mL 5.6763 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 4-Methylumbelliferone种透明质酸物合成抑制剂，具有抗肿瘤和

3、抗转移作。IC50 & Target hyaluronic acid 1体外研究4-Methylumbelliferone (4-MU) affects several key steps of angiogenesis, including endothelial cell1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEproliferation, adhesion, tube formation, and extracellular matrix remodeling. Half-maximal inhibitoryconcentratio

4、ns (IC50) values in the proliferation assay are 0.650.04 and 0.370.03 mM for HMEC and RF-24 endothelial cells, respectively. 4-Methylumbelliferone (2 mM) treatment for 24 h induces apoptosis in 13%of HMEC and 5% of RF-24 cells. The number of adherent endothelial cells decreases by 20% after 24 h oft

5、reatment with 1 mM 4-Methylumbelliferone. Minimal inhibitory concentrations in the tube formation assay are2 and 0.5 mM 4-Methylumbelliferone for HMEC and RF-24, respectively. Matrix metalloproteinase-2expression is differentially altered upon 4-Methylumbelliferone treatment in both tested endotheli

6、al cell lines1.PROTOCOLCell Assay 1 The MTT dye reduction assay in 96-well microplates is used. The assay is dependent on the reduction ofMTT by mitochondrial dehydrogenases of viable cells to a blue formazan product, which can be measuredspectrophotometrically. Endothelial cells (2.5103 cells in a

7、total volume of 100 L of complete medium) areincubated in each well with serial dilutions of 4-Methylumbelliferone (4-MU) (0, 0.5, 1, 1.5 and 2 mM). After 3days of incubation in the dark (37C, 5% CO2 in a humid atmosphere), 10 L of MTT (5 mg/mL in PBS) isadded to each well, and the plate is incubate

8、d for further a 4 h (37C). The formazan is dissolved in 150 L of0.04 N HCl-2 propanol, and samples are spectrophotometrically measured at 550 nm. All determinations arecarried out in quadruplicate, and at least three independent experiments are carried out. IC50 values arecalculated as those concent

9、rations of compound yielding 50% cell survival, taking the values obtained forcontrol as 100% 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Garca-Vilas JA, et al. 4-methylumbelliferone inhibits angiogenesis in vitro and in vivo. J Agric Food Chem. 2013 May 1;61(17):4063-71.McePdfHeightCaution: Product has not been fully validated for med