BAPTA _Reactive Oxygen Species 抑制剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEBAPTACat. No.: HY-100168CAS No.: 85233-19-8分式: CHNO分量: 476.43作靶点: ROS作通路: Protein Tyrosine Kinase/RTK储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 155 mg/mL (325.34 mM; Need ultrasonic)Ma

2、ss Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.0989 mL 10.4947 mL 20.9894 mL5 mM 0.4198 mL 2.0989 mL 4.1979 mL10 mM 0.2099 mL 1.0495 mL 2.0989 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的

3、百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.58 mg/mL (5.42 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.58 mg/mL (5.42 mM); Clear solution3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.58 mg/mL (5.42 mM); Clear sol

4、ution1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEBIOLOGICAL ACTIVITY物活性 BAPTA种钙 (calcium) 螯合剂。BAPTA 抑制细胞内活性氧 (ROS) 平。IC50 & Target Ca2+ chelator 1体外研究 Regarding ROS generation, a Ca2+ specific chelator, BAPTA, suppresses ROS generation of Sodium laurylsulfate (SLS)-exposed HaCaT keratinocytes 1

5、. Depolarization does not increase the resting open probabilityof the mechanoelectrical transducer (MET) current of Tmc1Bth/Bth OHCs, whereas raising the intracellularconcentration of the Ca2+ chelator BAPTA causes smaller increases in resting open probability in Bthmutantouter hair cells (OHCs) tha

6、n in wild-type control cells. In the presence of 0.1 mM BAPTA, nonsaturatingbundle displacements causes the MET current to adapt in both genotypes, exactly as seen when 1 mMEGTA is used in the intracellular solution. In the presence of 10 mM intracellular BAPTA, the time-dependentMET current decline

7、 is abolished and the resting Popen increased to near 50% of the maximal MET currentin OHCs from both Tmc1+/+ and Tmc1Bth/Bth mice. The relation between the MET current and bundledisplacement shows that increasing the intracellular BAPTA concentration from 0.1 to 10 mM significantlyincreased (p+/+ (

8、0.1 mM, 81.6%, n=4; 10 mM, 39.62.7%, n=5) and Tmc1Bth/Bth (0.1 mM, 10.42.2%,n=3; 10 mM, 46.59.9%, n=6). No significant differences are seen between the two genotypes for bothBAPTA concentrations. However, 3 and 5 mM BAPTA are less effective in shifting the MET current-bundledisplacement curves in Tm

9、c1Bth/Bth than in Tmc1+/+ OHCs. In Tmc1+/+, increasing the BAPTAconcentration from 0.1 mM to either 3 or 5 mM produces a highly significant increase in Popen (post hoc testfrom one-way ANOVA, pBth/Bth, the same comparison produced no or a much reduced increase in Popen(n.s. and p 2.户使本产品发表的科研献 Sci I

10、mmunol. 2019 Jun 28;4(36). pii: eaau6426.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Mizutani T, et al. Sodium Lauryl Sulfate Stimulates the Generation of Reactive Oxygen Species through Interactions with CellMembranes. J Oleo Sci. 2016 Dec 1;65(12):993-1001.2. Corns LF, et al. Tmc1 Point Mutation Affects Ca2+ Sensitivity and Block by Dihydrostreptomycin of the Mechanoelectrical TransducerCurrent of Mouse Outer Hair Cells. J Neurosci. 2016 Jan 13;36(2):336-49.McePdfHeightCaution: Product has not been