PACAP-1-38-human-ovine-rat-Pituitary-Adenylate-Cyclase-Activating-Polypeptide-38-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEPACAP (1-38), human, ovine, ratCat. No.: HY-P0221CAS No.: 137061-48-4Synonyms: Pituitary Adenylate Cyclase Activating Polypeptide 38分式: CHNOS分量: 4534.26Sequence: His-Ser-Asp-Gly-Ile-Phe-Thr-Asp-Ser-Tyr-Ser-Arg-Tyr-Arg-Lys-Gln-Me

2、t-Ala-Val-Lys-Lys-Tyr-Leu-Ala-Ala-Val-Leu-Gly-Lys-Arg-Tyr-Lys-Gln-Arg-Val-Lys-Asn-Lys-NH2Sequence Shortening: HSDGIFTDSYSRYRKQMAVKKYLAAVLGKRYKQRVKNK-NH2作靶点: Others作通路: Others储存式: Please store the product under the recommended conditions inthe COA.BIOLOGICAL ACTIVITY物活性 PACAP (1-38), human, ovine, ra

3、t种含有 38 个氨基酸残基的神经肽。PACAP (1-38) 结合到 PACAP I 型受体，PACAP II 型受体 VIP1 和 PACAP II 型受体 VIP2，IC50 分别为 4 nM，2 nM 和 1 nM。IC50 & Target IC50: 4 nM (PACAP type I receptor), 2 nM (PACAP type II receptor VIP1), and 1 nM (PACAP type II receptorVIP2) 1体外研究 PACAP (1-38), human, ovine, rat is a fragment of pituitary

4、 adenylate cyclase activating polypeptide 1.PACAP (1-38) shows high affinity for PACAP specific (PAC1) receptor in membranes from various tissuesincluding the endocrine pancreas 2. In vitro, PACAP (1-38) relaxes guinea-pig and rabbit tracheal smoothmuscle precontracted by histamine and by acetylchol

5、ine. PACAP (1-38) also increases adenosine 3:5-cyclicmonophosphate (cyclic AMP) in tracheal smooth muscle, providing a possible mechanism for the relaxanteffect of PACAP (1-38) 3.体内研究PACAP (1-38) alone in sham animals does not result in changes in any of the retinal layers. PACAP (1-38)dissolved in

6、solutio ophthalmica cum benzalkonio leads to significant protection in the retina in bilateralcommon carotid artery occlusion (BCCAO)-lesioned retinas; retinas treated with PACAP (1-38) eye drops1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEhave preserved structure compared to control retinas. OL

7、M-ILM (outer limiting membrane-inner limitingmembrane) distance is reduced by 49.7% (p 4.PROTOCOLAnimal Rats 4Administration 4 Wistar rats (n=20:n=12 for histological analysis, n=8 for immunohistochemical analysis) weighing 250-300are fed and watered ad libitum, under light/dark cycles of 12/12 h. D

8、irectly after the operation within 1 min,the right eye is treated with PACAP (1-38) eye drops (1 g/drop). The vehicle used is benzalkonium-chloridein a concentration of 0.005%, as it is the most effective vehicle to achieve neuroprotection with PACAP1-27eye drops. The left eye serves as a control, t

9、reated only with the vehicle. A group of animals serve as thesham-operated group that undergo anesthesia and all steps of the surgical procedure except ligation of thecarotid arteries. Rats are treated twice a day with one drop, for 5 consecutive days 4.MCE has not independently confirmed the accura

10、cy of these methods. They are for reference only.REFERENCES1. Gourlet P, et al. Fragments of pituitary adenylate cyclase activating polypeptide discriminate between type I and II recombinantreceptors. Eur J Pharmacol. 1995 Dec 4;287(1):7-11.2. Yamaguchi N. Pituitary adenylate cyclase activating poly

11、peptide enhances glucose-evoked insulin secretion in the canine pancreas invivo. JOP. 2001 Sep;2(5):306-16.3. Lindn A, et al. Inhibition of bronchoconstriction by pituitary adenylate cyclase activating polypeptide (PACAP 1-27) in guinea-pigs invivo. Br J Pharmacol. 1995 Jul;115(6):913-6.4. Werling D, et al. Passage through the Ocular Barriers and Beneficial Effects in Retinal Ischemia of Topical Application of PACAP (1-38)in Rodents. Int J Mol Sci. 2017 Mar 21;18(3). pii: E675.McePdfHeightCaution: Product