Dansylcadaverine-Monodansyl-cadaverine-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEDansylcadaverineCat. No.: HY-D1027CAS No.: 10121-91-2Synonyms: Monodansyl cadaverine分式: CHNOS分量: 335.46作靶点: Others作通路: Others储存式: -20C, protect from light, stored under nitrogen* In solvent : -80C, 6 months; -20C, 1 month (prote

2、ct fromlight, stored under nitrogen)溶解性数据体外实验 DMSO : 62.5 mg/mL (186.31 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.9810 mL 14.9049 mL 29.8098 mL5 mM 0.5962 mL 2.9810 mL 5.9620 mL10 mM 0.2981 mL 1.4905 mL 2.9810 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL AC

3、TIVITY物活性 Dansylcadaverine种荧光探针。体外研究Detection of Dansylcadaverine fluorescence after SDSshows that the amount of actin-thymosin 4crosslink is very low in the presence of Dansylcadaverine and transglutaminase, while there are largeamounts of Dansylcadaverine-labeled thymosin 4 and actin. The amount o

4、f cross-link further decreases inthe presence of Dansylcadaverine competing for Lys residues of either G-actin or thymosin 4 (100 M). Inthe absence of thymosin 4, Dansylcadaverine-labeled actin polymerized to F-actin as revealed by1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEfluorescence detecti

5、on after native PAGE 1.PROTOCOLKinase Assay 1 To determine the time course of transglutamination, thymosin 4 (120 M) is incubated withDansylcadaverine (5 mM) in 70 L buffer consisting of 10 mM Tris-HCl, pH 7.4, 15 mM CaCl2, 3 mM DTT.The reaction is started by addition of 0.1 U transglutaminase. Imme

6、diately after addition of the enzyme (t=0)and at indicated times,10 L are taken from the mixture, diluted in 490 L 0.1% TFA to stop the reaction andanalyzed by HPLC 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Cell Assay MCF7 cells (2.4104) are see

7、ded into 35 mm plates. After 24 h incubation, CuO NPs are added with anincreasing concentration in the presence or absence of 3-Methyladenine (3-MA) for different time periods .The cells are then incubated with 50 mM Dansylcadaverine (MDC) at 37C for 15 min and washed with1PBS three times with 5 min

8、 interval. Finally, the cells are observed under a fluorescence microscope 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Huff T, et al. Thymosin beta(4) serves as a glutaminyl substrate of transglutaminase. Labeling with fluorescentdans

9、ylcadaverine doesnot abolish interaction with G-actin. FEBS Lett. 1999 Dec 24;464(1-2):14-20.2. Laha D, et al. Interplay between autophagy and apoptosis mediated by copper oxide nanoparticles in human breast cancer cells MCF7.Biochim Biophys Acta. 2014 Jan;1840(1):1-9.McePdfHeightCaution: Product has not been fully validated for medica