RS-1 - RAD51 Activator - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemERS-1Cat. No.: HY-19793CAS No.: 312756-74-4分式: CHBrNOS分量: 524.23作靶点: RAD51; CRISPR/Cas9作通路: Cell Cycle/DNA Damage储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 100 mg/mL (190.76 mM)H2O : 40

2、% PEG300 5% Tween-80 45% salineSolubility: 3 mg/mL (5.72 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 3 mg/mL (5.72 mM); Clear solution1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEBIOLOGICAL ACTIVITY物活性 RS-1种 RAD51 的激活剂，同时可增强 CRISPR/Cas9 的活性。IC50 & Target RAD51 1, CRISPR/Cas

3、9 2体外研究 RS-1 is a RAD51 activator, stimulating binding of hRAD51 to DNA with Kd ranging from 48 nM to 107 nM inthe presence of ATP or ADP and in the absence of a nucleotide cofactor, and such an effect is not viainhibiting its ATPase activity. RS-1 (20 M) affects the length and helical pitch of hRAD

4、51 protein-DNAcomplexes. RS-1 (0, 1, 5, 10, 15, 20, and 25 M) stimulates strand assimilation activity of hRAD51. RS-1 (7.5M) promotes resistance of human cells to cross-linking chemotherapy 1. RS-1 (0, 7.5, 15 M) increasesCas9-mediated knock-in efficiencies in rabbit embryos 2.PROTOCOLKinase Assay 1

5、 Briefly, 15 L reaction volumes include a DNA strand exchange protein (0.8 M) that is preincubated for 5min at 37C with 1 M (nucleotide concentration) 32P-labeled oligonucleotide 306.7 in a reaction buffercontaining 20 mM Hepes (pH 7.5), 1 mM DTT, 2 mM nucleotide cofactor, and 1 mM MgCl2 and various

6、concentrations of RS-1. For experimental buffer conditions that included calcium, 1 mM CaCl2 is present inaddition to (in the case of hRAD51) or in the place of (in the case of RecA and scRAD51) the 1 mM MgCl2.Conditions with scRAD51 additionally contains 110 nM scRAD54. After this initial binding r

7、eaction, 10 L of19.75 M (base pair concentration) supercoiled homologuecontaining target plasmid DNA (pRS306) is nextadded along with sufficient magnesium acetate to give a final concentration of 10 mM 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户

8、使本产品发表的科研献 J Mol Med (Berl). 2019 Jun 14.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Jayathilaka K, et al. A chemical compound that stimulates the human homologous recombination protein RAD51. Proc Natl Acad Sci US A. 2008 Oct 14;105(41):15848-53.2. Song J, et al. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency. Nat Commun. 2016 Jan 28;7:10548.McePdfHeightCaution: Product has not been fully validated for medical applica