对多巴胺敏感的溶胶－凝胶－植物组织生物传感器的研制

1、.a-苹果汁-溶胶-凝胶修饰电极在EP中b-Nafion-苹果汁-溶胶-凝胶修饰电极在EP中c-Nafion-苹果汁-溶胶-凝胶修饰电极在DA和EP混标中5、分析运用配制一系列浓度的多巴胺规范溶液，采用示差脉冲伏安法及利用Nafion-苹果汁溶胶凝胶修饰电极对多巴胺规范溶液进展测定，绘制任务曲线图6。结果阐明，多巴胺浓度在210-6 mol/L110-4 mol/L范围内呼应电流呈良好的线性关系。汲取多巴胺针剂溶液30L，用PBS定容配成25mL即多巴胺浓度为6.3310-5 mol/L。用本传感器进展定量分析，测得多巴胺的浓度为6.5810-5 mol/L，实验误差为3.9%。参考文献1 蔡

2、沛祥，黄昊，余新志，聂兵，“对多巴胺敏感的植物组织碳糊生物电极的改良， 传感技术学报，1996,3, (1)：75-782 唐尹平，成凤桂，“Sol-Gel固定法制造生物传感器运用及开展，中南民族学院学报，2000，3，19(1):89-933 何星存，李平，莫金垣，“用修饰电极导数伏安法同时测定多巴胺和肾上腺素，分析测试学报，2000，1，19(1):19-214 汪振辉，郏建波，李工安，周漱萍，“多巴胺在聚茶碱Nafion双层修饰玻碳电极上的电化学行为，分析化学，2000,5,28(5):568-5725 邓家祺，方跃，“植物组织电极，化学传感器，1990，10(3)：5-106 沈国励，

3、陈光焰，水冰，“土豆组织膜儿茶酚电极的研讨，分析化学，1990，18(4)：315-319A Tissue Biosensor Based On Sol-gel Immobilization TechniqueXuan Jun, Chen Lixing, Xu Changliu(Class 2000, School of Chemistry and Chemical Engineering, Sun Advisor: Cai Peixiang, ProfessorAbstract：In this paper, a new technique on preparation of biosenso

4、r is reported. SiO2 sol-gel and plant juice are mixed a certain proportion to make the enzyme from nature plant immobilized in the SiO2 sol-gel film. The dopamine biosensor is prepared from a platinum electrode coated with a Nafion film and then the midified SiO2 sol-gel layer.Cyclic voltammetry(CV)

5、 and Linear scan voltammetry are used to investigate the electrochemical characteristics of the sensor. The catalysis effect of different plant species and parts is compared. The dosage of ethanol, water, TEOS and plant juice is studied. The linearity of DA in the range of 210-6110-4mol/L with detec

6、tion limit of 8.010-7mol/L and the correlation coefficient of 0.999 are obtained by differential pulse voltammetry(DPV). No interference was found from ascorbic acid, nitrite, uric acid, epinephrine and noradrenalin in the determination of DA. Satisfactory determination results of DA in samples are obtain by t