bankit提交序列方法

1、第一步：填好联系人信息需要填写姓名、部门、机构、地址、所在城市、所在省份、 、国家、联系 、 号码、邮箱、备用邮箱、第二步：Reference.序列作者姓名，可填多位序列作者.文章信息：填写文章的标题并选择文章的出版状态；是否与序列作者为同一人；参考文章也可多篇。cenoanK oucmissionsContact ReferenceSubmission # 2062762Sequence AuthorsFirst Name Middle Initial(s) Last Name Suffix RemoveI -l|l I 11=111 l| 以Add more sequence author

2、sReference Information #1Please provide the title and relevant publication details (volume, issue etc ) of a paper that discusses this submissionPUBLICATION STATUS Unpublished In-Press D Published Reference Title/REFERENCE AUTHORS1 Same As Sequence AuthorsSpecify New AuthorsAdd Another ReferenceCont

3、inue第三步：Sequencing Technology.当序列超过500个或者通过下一代测序技术测得序列那么需要填写 测序方法。.选择序列是否是组装序列。.如果是 assembled sequences，还需填写 assembly program,一般是填Lasergene,并写上相应的版本。ISequencing TechnologyThis information is required if you are submitting over 500 sequences or if your sequences were generated using next-generation s

4、equencing technology.What methods were used to obtain these sequences?L Sanger dideoxy sequencing 454HelicosIlluminaLlonTorrentPacific BiosciencesSOUDC OtherAre these sequence(s):0 unassembled sequence reads0 assembled sequences(consisting of two or more)sequence reads)ContinueWhat program(s) did yo

5、u use to assemble these sequences?Coverage:Continue注：Assembly Name和Coverage这两项不清楚要填什么。第四步：Nucleotide.期望NCBI什么时候公布你的序列.该序列是否为16s rRNA.填写或选择所提交序列的信息：包括分子类型，结构类型(线性环 形等).提交序列：可以直接复制黏贴序列，也可以创立FASTAfile上传。uenuanK uomissionsContact Reference Sequencing Technology NucleotideSubmission # 2062762Submission R

6、elease DateWhen may we release your sequence record? Immediately After Processing Release Date: |Date format is DD-Mon-YYYY (example: 20-Feb-2004)16S rRNA submissionsAre the sequences in this submission ONLY 16S ribosomal RNA data? Yes NoSequence(s) and Definition Line(s)Molecule Type:Linear Yes NoT

7、opology:Are you submitting the complete sequence of an organelle genome, virus, viral segment, viroid, plasmid, or cloning vector?Nucleotide Sequence(s) and Definition LinesSequences must be entered in the FASTA format, whether you are submitting a single sequence or multiple sequences Definition Li

8、nes which are used to describe each sequence should be included in the FASTA format.How many nucleotide sequences do you intend to send in this submission?Paste SequenceExample FASTA nucleotide format:Seql organism=genus species Definition Line for Seql aaccgatatagagagagga.Seq2 organism=genus specie

9、s Definition Line for Seq2atctgaatagagattattt.(OR)Upload FASTA fileUpload FASTA file选择文件未选择任何文件How do I create aFASTAfile?Continue第五步：Submission Category选择你所提交的序列是原始提交还是作为一个第三方注释的提交, 般自己发现的序列我们选的是原始提交。GenBank SubmissionsContact Reference Sequencing Technology Nucleotide Submission CategorySubmission

10、 # 2062762Submission CategoryIndicate whether your sequence is an original submission or a thidwarty annotation submission OriginalDirectly sequenced by submitter.Third Party Annotation Derived from other primary sequence dataContinue第六步：Source Modifiers （来源信息）第一项为哪一项细胞器/位置，可不填第二项可以在Source Modifiers

11、下拉列表选择你想要加以描述的参数种类，并在右边value栏写上相应描述。Contact Reference Sequencing Technology Nucleotide Submission Category Source ModifiersSubmission # 2062762Source ModifiersSource Organelle/Location InformationChoose the correct oqrnelle location, if applicableOrganelle/LocationSource ModifiersChoose source modif

12、ier(s) and enter value(s) herePlease refer to Source modifie忖 for description and formatsource modifiervalueRemoveAdd another source modifierAny other source related information?幽For example, Taxonomic Classification or Lineage for unknown/unrecognized organisms. You should create a Source Modifier

13、Table for notes or otl ,note* as the header label. Do not enter individual source modifiers as plain text here Choose them from the drop down list in the Source Modifier sIf you want to include PCR primers in your submission, check here:Continue第七步：Features (Overview)我们选择上传一个 five column feature tab

14、le file。five column feature table file 的格式是：.一共5列，用Tab符隔开.第一行：Feature Sequence_ID.每个feature单独成一行. 一个feature的多个nucleotide intervals在随后的行里.第一列：feature的起始位置第二列：feature的终止位置第三列：feature的名字第四列：Qualifier的名字，如note、codon_start等等第五列:Qualifier value,即Qualifier的具体描述举例：Feature Seql1080 1210 CDS1275 1315productac

15、tinnotealternatively spliced1055 1210 mRNA1275 1340productactin1055 1340 genegeneACT1055 1079 5UTR 1316 1340 3UTRSubmission a 2062762解Features (Overview)Please provide feature annotations for your submission by choosing one of the two options below Add features by uploading five column feature table

16、 fileAdd features by completing i叩ut formsThis method is more suitable for:adding many different features on a single sequence or on multiple sequencesuses the five-column tab-delimited feature table format which is also used in Sequineach table in the feature table file applies to only one sequence if multiple sequences have been uploaded in your nucleotide fasta file, each corresponding table must be labeled with that sequences Sequence IDmultiple tables can be uploaded in a single fileUploading multiple feature table files will result in the last file rep