5-BrdU-BUdR-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemE5-BrdUCat. No.: HY-15910CAS No.: 59-14-3Synonyms: BUdR; BRDU分式: CHBrNO分量: 307.1作靶点: Nucleoside Antimetabolite/Analog作通路: Cell Cycle/DNA Damage储存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect froml

2、ight)溶解性数据体外实验 DMSO : 41 mg/mL (133.51 mM)H2O : 33.33 mg/mL (108.53 mM; Need ultrasonic)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 3.2563 mL 16.2813 mL 32.5627 mL5 mM 0.6513 mL 3.2563 mL 6.5125 mL10 mM 0.3256 mL 1.6281 mL 3.2563 mL请根据产品在不同溶剂中的溶解度，选择合适的

3、溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 5-BrdU种核苷类似物，可与胸苷竞争掺DNA。5-BrdU通常于检测增殖细胞。体外研究Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stemcell population expansion RG2 rat glioma cells. In H9 cells and BJ fibroblasts, bromodeoxyuridine alters t

4、hecell cycle profile 1. BrdU is stably integrated into the DNA, and thus can be used in assessment of cell1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEproliferation and other cell procession 2.体内研究 Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/mL, p.o.) significantly slows tumor progression in ra

5、t gliomaRG2 tumor model 1.PROTOCOLCell Assay 1 Cultures are initially plated at 2000 cells/cm2 and are quantified with a Z2 Coulter Counter. RG2 rat gliomacells are treated once with 0, 1, 10, or 50 M BrdU for 24 hours, and cumulative growth curves are obtainedover 18 days. Control and treated cells

6、 are quantified and replated at equal densities on days 5, 12, and 18after treatment.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 Cell Res. 2019 Jan;29(1):23-41. EBioMedicine. 2018 Dec. J Mol Cell Cardiol. 2018 May;118:133-146. J Mol Med

7、(Berl). 2019 Jun 14. Obesity (Silver Spring). 2017 Sep;25(9):1532-1539.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Levkoff LH, et al. Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo. Neoplasia. 2008 Aug;10(8):804-16.2. Rothaeusler K, et al. Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry. Curr ProtocCytom. 2007 Apr;Chapter 7:Unit7.31McePdfHeightCaution: Product has not been fully validated for medical applications.For res