附表一生物安全等级规范及病原体微生物危险性等级分类

1、 附表生物安全等级祝靶及病原醴微生物危陂性等级分I危陂群典生物安全等级、操作规靶及段借封照表危陂群RISK GROUP生物安全等 敬 BIOSAFETYLEVEL室型LABORATORYTYPE室操作规靶LABORATORY PRACTICES安全同咻ISAFETY EQUIPMENT第一级1第一等级 Biosafety Leve1基磁教孥、研究 Basic teaching, research便良微生物孥技衍GMT瓢，K放式工作椁None; open bench worl第二级2第二等级 Biosafety Leve2初级衢生服矜、 段断服矜、研究 Primary health servic

2、es; diagnost services, research便良微生物孥技衍 加上防港衣、生物危 害襟旨杰G MT plus protectiv clothing, biohazard siK放式工作椁加上防 止氧U外流之生物安 全檀Open bench plus BSC potential aerosolsor第三级3第三等级 Biosafety Leve3特殊服矜、 研究Special diagnostic services, research同第二等级加上特 殊防诙衣、迤入管制 及定向氟流As Level 2 plus speci clothing, controlled access

3、, directional airflow生物安全檀及（或）其 他所有If瞬室工作所 需要之基本装置B SC and/or other prima devices for all activitiesiry第四级4第四等级Biosafety Leve4具危陂性之病原Dangerous pathogen units同第三等级加上氧 密因、出口淋浴及JS 粢物之特殊虑理As Level 3 plus airlock entry, shower exit special waste dispose三级生物安全檀或二 级生物安全檀穿著 正屋防衣、1!因高屋 :烝氟力戒菌器(穿精式) 及谩滤之空氟Cla

4、ss III BSC, or positiv pressure suits in conjunctionwith Class II BSCs, double-ende autoclave (through the wall), filtered aired病原微生物依其危性悬基磁之分m（依at行政院阈家科孥委n曾九十三年六月增修之基因重黜守刖）甲、第一级危陂群（Risk group 1 , RG1微生物第一级危陂群微生物典人I健康成人之疾病瓢 。例如不生内抱子 之 BacilluslicheniformisEscherichiacol-K12, adeno-associateVirus第型至第

5、 四型，及重黜的不含潸在致癌基因物或毒素之 adeno-associated virusH 在瓢轴助病毒存在下迤行培餐。乙、第二级危陂群（Risk group 2 , RG2微生物第二级危陂群微生物在人I所引起的疾病很少是殿重的，而且通常有 55防及治瘵的方法。（一）余田菌包括披衣菌1Acinetobacter baumanni以前耦悬 Acinetobacter calcoacetiCus2.Actinobacillus3Actinomyces pyogenestuSI Corynebacterium pyogenes.Aeromonas hydrophila.Amycolata autot

6、rophica6Archanobacterium haem01yticum前？ Corynebacterium haemolyticum7Arizona hinshaw 口所有血清型）.Bacillus anthracis.Bartonella henselae, B. quintana, B. vinsonii10Bordetellsfe括 B. pertussis1.Borrelia recurrentis, B. burgdorferi12Burkholderia（以前旗& Pseudomonas specie冽於第三级危陂群者除外）.Campylobacter coli, C. fet

7、us, C. jejuni.Chlamydia psittaci, C. trachomatis, C. pneumoniae.Clostridiumbotulinum,Cl. chauvoei,Cl. haemolyticumC, l. histolyticum,Cl. novyi,Cl. septicum, Cl. tetani.Corynebacterium diphtheriae, C. pseudotuberculosis, C. renale.Dermatophilus congolensis.Edwardsiella tarda.Erysipelothrix rhusiopath

8、iae20Escherichia co口所有踢致病性、踢致毒性、踢侵H性及揣带有K1抗原的菌株，包括E. coliO157： H7)21.Haemophilus ducreyi, H. influenzae22.Helicobacter pylori23Klebsiella(除 K. oxytoca(RGI)以外之所有菌株)24Legionell血括 L. pneumophila25Leptospira interroga nSff 有血清型)26.Listeria27.Moraxella28Mycobacterium (除了列於第三级危陂群者)包括 M. avium complex, M.

9、asiaticum,M. bovis BCG vaccinestrain, M. chelonei,M. fortuitum,M. kansasii,M. leprae, M. malmoense, M. marinum, M. paratuberculosis, M. scrofulaceum, M. simiae, M. szulgai, M. ulcerans, M. xenopi29Mycoplasma(M. mycoidesS M. agalactia除外).Neisseria gonorrhoeae, N. meningitidis.Nocardia asteroides, N.

10、brasiliensis, N. otitidiscaviarum, N. transvaiensis.Rhodococcus equi33.Salmonell血括 S. arizonae, S. cholerasuis, S. enteritidis, S. gallinarum-pullorum, S. meleagridis, S. paraty,phAi, B, C,S. typhi, S. typhimurium34.Shigell胞括 S. boydii, S. dysenteriae, typeS. flexneri, S. sonnei35.Sphaerophorus necr

11、ophorus36.Staphylococcus aureus37.Streptobacillus moniliformis38.StreptococcusL括 S. pneumoniae, S. pyogenes.Treponema pallidum, T. carateum.Vibrio cholerae, V. parahemolyticus, V. vulnificus.Yersinia enterocolitica(二)真菌1.Blastomyces dermatitidis.Cladosporium bantianum(, CX. ylohyph)a trichoides.Cryp

12、tococcus neoformans.Dactylaria galopa(vaOchroconis gallopavu) m.Epidermophyton.Exophiala( Wangiell)a dermatitidis7Fonsecaea pedrosoi8.Microsporum.Paracoccidioides braziliensis.Penicillium marneffei.Sporothrix schenckii12.Trichophyton(三)寄生盘lAncylostomhuman hookwormS括 A. duodenale, A. ceylanicum2Ascar

13、isfe括 Ascaris lumbricoides suumBabesi胞括 B. divergens, B. microtiBrugiafilaria worms!括 B. malayi, B. timori5.CoccidiaCryptosporidiurfe括 C. parvumCysticercus cellulos(aehydatid cyst, larva oT.f solium)Echinococcusl!括 E. granulosis, E. multilocularis, E. vogeli9.Entamoeba histolytica10.EnterobiusIIFasc

14、iol胞括 F. gigantica, F. hepatica12Giardiat!括 G. lamblia13.Heterophyes14Hymenolepife括 H. diminuta, H. nana15.Isospora16Leishmani包括 L. braziliensisL. donovani,L. ethiopia,L. major, L. mexicana,L. peruvania, L. tropica.Loa loafilaria worms.Microsporidium.Naegleria fowleri20Necatorhuman hookworms括 N. ame

15、ricanus21 Onchocercfilaria worms!括 O. volvulus22Plasmodiumfe括引起猿猴I疾病之 P. cynomologip. falciparumP malariae,P ovale, P.vivax23.Sarcocysti鱼括 S. sui hominis24.SchistosomOl括 S. haematobiumS. intercalatum,S. japonicum,S. mansoni,S. mekongi25.Strongyloidesl括 S. stercoralis26.Taenia solium27Toxocan01 括 T.

16、canis28Toxoplasm01 括 T. gondii29.Trichinella spiralis30Trypanosomfe括 T. brucei brucei, T. brucei gambiense, T. brucei rhodesiense, T. cruzi31.Wuchereria bancroffitliaria worms(四)病毒1.Adenoviruses, human-all types2.ArenavirusesLymphocytic choriomeningitis v( irunson-neurotropic strai) nsTacaribe virus

17、 complexc.其他被特别引述而列名之病毒3.BunyavirusesBunyamwera virusRift Valley fever virus vaccine strain MP-12c.其他被特别引述而列名之病毒4.Calciviruses.Coronaviruse s SARS-Associated Coronaviruse舱第三级危陂群除外).Flaviviruse(s Togaviruse) s - Group B ArbovirusesDengue virus serotypes 1, 2, 3, and 4Japanese encephalitis virusYellow

18、 fever virus vaccine strain 17Dd.其他被特别引述而列名之病毒.Hepatitis A, B, C, D, and E viruses.Herpesviruses Herpesvirus simiaeMonkey B virUs 除外)CytomegalovirusEpstein Barr virusHerpes simpletxypes 1 and 2Herpes zosterHuman herpesvirus types 6 and 79.Orthomyxovirusesa. Influenza viruses types A, B, and Cb.其他被特别

19、引述而列名之壁赢揣带正黏液病毒 10.PapovavirusesAll human papilloma viruses.ParamyxovirusesNewcastle disease virusMeasles virusMumps virusParainfluenza viruses types 1, 2, 3, and 4Respiratory syncytial virus.ParvovirusesHuman parvoviru( sB19).PicornavirusesCoxsackie viruses types A and BEchoviruses-all typesRhinovi

20、ruses-all types.Poxviruses (Monkeypox viruS被管制的 Alastrim, SmallpoX WhitepoXB外).Reovirusefe括 Coltivirus, human RotavirUSOrbivirus(Colorado tick fever virus 16.RhabdovirusesRabies virus-all strainsVesicular stomatitis vii籁室调逋的病毒株包括 VSV-Indiana, San Juan Glasgow17.Togaviruses#JH Alphavirusesi Flaviviru

21、sesRubiviru(s rubella)丙、第三级危(t群(Risk group 3 , RG3微生物第三级危陂群微生物在人IS可以引起殿重或致死的疾病，可能有55防 及治瘵之方法。(一林田菌包括立克次氏菌1.Bartonella2.Brucell胞括 B. abortus, B. canis, B. suis3.Burkholderi(a Pseudomona) s mallei, B. pseudomallei.Coxiella burnetii.Francisella tularensis.Mycobacterium bovis但 BCG 除外)，M. tuberculosis.Pa

22、steurella multocidtyape B - buffalo and other virulent strains.Rickettsiaakari, R. australis,R. canada, R. conorii, R. prowazekii,R. rickettsii,R.siberica, R. tsutsugamushi, R. t(ypRhi. moose)ri.Yersinia pestis(二 )真菌.Coccidioides immi(tissporulating cultur；escontaminated so) il.Histoplasma capsulatu

23、m, H. capsulatum var. duboisii(三)寄生n妞八、(四)病毒典建性蛋白(PrionS.Alphaviruse( s Togaviruse) s - Group A ArbovirusesEastern equine encephalomyelitis virusSemliki Forest virusSt. Louis encephalitis virusd.Venezuelan equine encephalomyelitis VirUsTC-83疫苗株)e. Western equine encephalomyelitis virusf.其他被特别引述而列名之病

24、毒2.ArenavirusesFlexalLymphocytic choriomeningitis v( iruLsCM) ( neurotropic strai)ns3.BunyavirusesHantaviruse包括 Hantaan virusRift Valley fever virus4.CoronavirusesSARS-associated Coronaviruses (SARS-CoV)l-(瞬操作必须殿格遵照本守刖附表八之规定5.Flaviviruse( s Togaviruse) s - Group B Arbovirusesa. West Nile VirusYellow

25、 fever virusc.其他被特别引述而列名之病毒6.Picornavirusesa. Polioviruses-all types, wild and attenuated7.PoxvirusesMonkeypox virus8.PrionsTransmissible spongioform encephalop( atThSieEs) agen(ts Creutzfeldt-Jacob disease and kuru agen）ts.RetrovirusesHuman immunodeficiency v（iruHsIV） types 1 and 2Human T cell lymp

26、hotropic v（ iruHsTLV） types 1 and 2Simian immunodeficiency v（iruSsIV）.RhabdovirusesVesicular stomatitis virus丁、第四级危群（Risk group 4 , RG4微生物第四级危陂群微生物在人IS可以引起殿重或致死的疾病，但通常0 防及治瘵之方法。（一）余田菌妞 八、（二 ）真菌妞 八、（三）寄生盘妞 八、（四 ）病毒.ArenavirusesGuanarito virusLassa virusJunin virusMachupo virusSabia2.Bunyaviruse（ s Na

27、iroviru）sCrimean-Congo hemorrhagic fever virus3.FilovirusesEbola virusMarburg virus.Flaviruse（s Togaviruse） s - Group B ArbovirusesTick-borneencephalitisvirus complext!括 AbsetterovCentralEuropeanencephalitis, Hanzalova,Hypr,Kumlinge,KyasanurForestdisease,Omskhemorrhagicfever 及 Russian spring-summer

28、encephalitis viruses.Herpesviruse（ s alpha）Herpesvirus simiaeHerpes 嗽 Monkey B virUs.ParamyxovirusesEquine morbillivirus.Hemorrhagic fever agents and viruses as yet undefined戊、通用的S&物病原性病毒下列室中常用的勤物病原性病毒典健康成人之疾病瓢 。通常RG1 80勺防K等级即可。但封 murine leukemia virus amphotropi汲 xenotropiM毒 株，建Si於RG2级之防等级下迤行1.Bacu

29、loviruses2.HerpesvirusesHerpesvirus atelesHerpesvirus saimiriMarek s disease virusMurine cytomegalovirus3.PapovavirusesBovine papilloma virusPolyoma virusShope papilloma virusSimian virus 4（ 0SV40）4.RetrovirusesAvian leukosis virusAvian sarcoma virusBovine leukemia virusFeline leukemia virusFeline s

30、arcoma virusGibbon leukemia virusMason-Pfizer monkey virusMouse mammary tumor virusMurine leukemia virusMurine sarcoma virusRat leukemia virus附表二 生物安全意外事件等级明、通幸91定及虞理意外等级茨:明通幸魅1定虑理第一级意外彝生於室 防内（例如在 操作畤，具高感 染性梅Tl1翻濯於生 物安全檀中），/、致 封人H生命造成立 即性危害。J8向室主管幸艮 告亚ill面百己金泉。依1S置罩位之室生物安全聚急J8建言十重内容（生物安 全意外事件虑理程序）虑理。第二级意外彝生於室 防ms借外之其他 K域（例如拿取局感 染性横不慎掉落 室地板，造成溅 ）,封人jM生命及室内部琪境有 危害及污染之虞。J8向室主管幸艮 告或善面