genius水冷ndyag激光文献选辑

1、信Author: AA WAvander AJvanGAvanderNd:YAG 激光信Author: AA WAvander AJvanGAvanderNd:YAG 激光非手术疗法在老年患者中的应用作者：向学应用Nd:YAG 激光减轻牙周刮治术中疼痛的临床观作者段向Nd:YAG 水激光在广泛型侵袭性非手术治疗的作者向学的短期临床疗效观作者叶 学Nd:YAGlaserclinicalassistedinclassIIfurcationAuthor:Ana Karina odeAndrade&IlriaSalomoFeist&CludioMendesPannutiSilvanaCai&Denis

2、eMariaZezell&ioDeLong-termeffectsofasingleapplicationofawater-cooledNd:YAGlaserinsupplementtoscalingandrootplaninginpatientswith periodontal inflammationAuthor:TalatQadri&FawadJaved&PavlinaPoddani&JanTunr&EvaluationofsurgicaltreatmentofperiodontalpocketswithRoyal Dental College,University 信致阅我，Thork

3、ildKarring, 是奥胡斯大。本次合作研究的合作伙伴包括了牙、Genius 激光公司经过奥胡斯牙以及丹麦贸易洋行的努力确认了经过多次改水冷激光系统是信致阅我，ThorkildKarring, 是奥胡斯大。本次合作研究的合作伙伴包括了牙、Genius 激光公司经过奥胡斯牙以及丹麦贸易洋行的努力确认了经过多次改水冷激光系统是牙周治疗的合作设备，原因如率的切，同时保持水/气喷雾专利的冷却效果使得：激光可以长时间作用于组织；可忽略不计的气彻底避免软水冷激光专为牙周治疗设计，同时配合严谨的培训教育体系Genius 水冷激光的优点是：水/气喷雾专利，保证独一无二的优化激光参数可以无无痛的切割为了获得

4、最佳的切割效果，必须尽量缩短脉冲的持续时间（等同保证安全无痛和无血手术，需要对软组织有一血，同时短到保持足够的切割效果是组织处于持续冷却环Genius 水冷激光经过奥胡斯大牙 1、水冷Nd-YAG 激光对牙周袋龈下洁2、水冷Nd-YAG 激光对牙周袋手术洁最终：Thorkild Karring 教授认为此两项研究表明了激光治疗获得的临床改善同常规方水/气瀑布配合下的Genius 水冷激光水/气瀑布配合下的 Genius 水冷激光是专利设备，水从光纤的同一管道中传输。这Genius 水冷激光采用水/气瀑布的目的enus水冷激光不但有热效应，同时还有可控制的等离子消融再生修复术效应。水/气水/气瀑

5、布消除了组织碳化、预防了热传导对周围组织优化参数、使用水/气瀑布配合的激光，理所当然是牙周治疗的好方Genius 水冷激光就有这样优异表现：无痛、切割效率、没有组织碳化；同时保持完ThorkildKarring 口腔及牙周丹麦奥胡斯牙SHORT COMMUNICATIONAA Kranendonk AJvanWinkelhoff GAvanderThe bactericidal effect of Nd:YAG Authors A.A.Kranendonk,G.A.vanderWeijden, Department of Periodontology, Academic Centre for

6、Dentistry Amsterdam, Amsterdam, The NetherlandsA.A.Kranendonk,G.A.vanderWeijden,Clinic for Periodontology, Utrecht,The W.A.vanderSHORT COMMUNICATIONAA Kranendonk AJvanWinkelhoff GAvanderThe bactericidal effect of Nd:YAG Authors A.A.Kranendonk,G.A.vanderWeijden, Department of Periodontology, Academic

7、 Centre for Dentistry Amsterdam, Amsterdam, The NetherlandsA.A.Kranendonk,G.A.vanderWeijden,Clinic for Periodontology, Utrecht,The W.A.vanderReijden,A.J.vanWinkelhoff, Department of Oral Microbiology, Academic Centre for Dentistry Amsterdam, Amsterdam, The Netherlands: e: To evaluate the in vitro ef

8、fectoftheNd:YAGlaser(Genius,MlsgaardCopenhagen,Denmark)onsixperiodontalMethods:sofsixdifferentperiodontalitans,gingivalis,Prevo ermedia,TannerellaFusobacteriumnucleatumandParvimonasmicra)preparedinsmallEppendorfftubes,andedto Nd:YAGlaserforfivedifferentperiodsoftime.Laserer6 Watt(onascaleof112W), 50

9、Hz,Pulseduration250ls.AfteruretotheCorrespondence FridusvanderDepartment of AcademicCentreforDentistryAmsterdam Louwesweg 11066EA Amsterdam The Netherlands.: +31-20-51 88 Fax: +31-20-51 88 : HYPERLINK mailto:ga.vd.weijdenacta.nl aliquots of the s were spread on blood platesforbacterialcounting. Resu

10、lts:After5sofure,therewasaotalcolonyformingforallsixselectedanisms.After15,30and45s,acterial cells could be :invitro,15sofNd:YAGlaserusewasfoundtoeffectivefortotalkillingofthesixtestedKey words: in vitro; laser; anisms; Accepted 22 September Most treatment modalities used in periodontal therapy aim

11、reduce the bacterial plaque on the root surface to levels ible with periodontal health. The effectiveness of scaling and root planing in the treatment of periodontal disease is TocitethisJDentHygiene8,2010;: 10.1111/j.1601-KranendonkAA,vanderReijdenWA,vansally accepted (1, 2). Some studies periodont

12、al treatment is better if particular can no longer be detected after therapy andt e ed t sites which breakdown J,vanderWeijdenGA.TheeffectofaGenius Nd:YAG2009 The Journal compilation 2009 Blackwell itive are at greater risk of Laser s ique t make it useful JDentHygiene8,2010;Kranendonk et al. Bacter

13、icidal effect of a Nd:YAG a variety of applications in dental care. It is capable of In vitro Small Eppendorff tubes were used in an in vitro experiment to study the microbiological effects of laser treatment on the killing of periodontal pathogens. Fresh clinical isolates of Aggregatibacter itans,

14、Porphyromonas gingivalis, Prevo ladia, Tannerella forsythia, Fusobacterium nucleatum and Pa and vapourizing plaque, and has the sulcular lining (46). aims atKranendonk et al. Bactericidal effect of a Nd:YAG a variety of applications in dental care. It is capable of In vitro Small Eppendorff tubes we

15、re used in an in vitro experiment to study the microbiological effects of laser treatment on the killing of periodontal pathogens. Fresh clinical isolates of Aggregatibacter itans, Porphyromonas gingivalis, Prevo ladia, Tannerella forsythia, Fusobacterium nucleatum and Pa and vapourizing plaque, and

16、 has the sulcular lining (46). aims at reducing or eliminating be clinically useful to reduber of authors have n. A cidal effect of ight is one of the eraction. The high tal of neodymium-tlaser energy (4, 711). Absorption of micra were retrieved from subgingiva patients with chronic periodwere les m

17、ost important factors in doped yttrium aluminium laser emitting light is well ab bin. The energy is tran absorbed in hydroxyapitto use in a period vascularized studi transmitted wiemedium, pectrum. This haemin (5 mg l)1) and menadione (1 mg l)1) and incubated for 14 days at 37 C in 80% N2, 10% CO2 a

18、nd 10% H2 (13). Identifi- cationwasbasedonGramstain,cellandcolonymorphology, air tolerance, production of catalase and on a number of dcal reactions. tryptic soy-itanswas isolated on ycin (TSBV) plates bated in air with 5% CO2 at 37 C for 3 days (14). Isolates identified onthe basis ofcharacteristic

19、 colony morphologyn t nto s the like inner structure) on TSBV, itive catalase reaction the characteristics of laser light, direct and the fibre tip is not required 3% hydrogen peroxide and a set of specific enzymes LaBalmelesGrottes,Monta u-Vercieu,by et al. dentists and Bacterial 72 h and were sub-

20、cultured on blood agar base s were prepared in phosphate-he Netherlands, the water cooled Nd:Yag laser is used as an adjunct to the -surgical y 105 colony forming units per ml. ul in an Eppendorff PCR-vial. saline of test volume was ment of periodontitis, as has been suggested by etal.starting the e

21、xperiment, 20 ll was extracted and spread on The e of this study was to investigate the culture plate for a baseline performed in triplicate for each Measurements dal effect of ius Nd:YAG laser (Genius anism ure time. Before use, the laser ivated on a of carbon r. Bacterial s were prepared to AS, Ml

22、sgaard Dental, Copenhagen, by ed to a Nd:YAG laser for four different periods of mining the time nsary to kill MaterialandA solid s e crystal Nd:YAG laser (Genius Periodontal A S) was used at the settings, as shown in Table 1. The Genius Nd:YAG laser with water and air coolant has an optical fibre t

23、ip, approxima y the size of riodontal probe (0.6 mm in Table 1. Nd:YAGlaser600112 112 112 10100 100800 400800 Pulse energyFig. 1. Laser tip (on the left) o the tube (on the right) which was followed by microbial JDentHygiene8,2010;Kranendonk et al. Bactericidal effect of a Nd:YAG Table 2. Presence (

24、+) or absence () of cultivable microbiologicalspeciesonthecultureplatesrelatedtotheried out through -surgical scaling and root planing, or by surgical procedures aimed at the elimination of pockets. Furthermore, antibiotics and antiseptics are used as an adjunctive to mechanical debridement to obtai

25、n elimination of certain bacterial species.differenturetimeoftheNd:YAGlaserin ure time 5+)+)+)+)+)+)More recently, it has been Kranendonk et al. Bactericidal effect of a Nd:YAG Table 2. Presence (+) or absence () of cultivable microbiologicalspeciesonthecultureplatesrelatedtotheried out through -sur

26、gical scaling and root planing, or by surgical procedures aimed at the elimination of pockets. Furthermore, antibiotics and antiseptics are used as an adjunctive to mechanical debridement to obtain elimination of certain bacterial species.differenturetimeoftheNd:YAGlaserin ure time 5+)+)+)+)+)+)More

27、 recently, it has been t er suchastheNd:YAGlaser,maybeusefulfordestroyingpresumably by a thermal effect (9, 17, 18). The erNd:YAGlaseronaofactionofachia coli was studied. In a thermocouple equipped cuvette, a temperature rise up to 50 C was observed after the use of Aa,Aggregatibacter gingivalis; Pi

28、, itans;Pg,Porphyromonas ermedia; Tf, Tannerella forsythia;Pm,Parvimonasmicra;Fn,Fusobacteriumer output of 100 W for 23 s. This n 90%loss ofviability ofE.coli.Asa comparison,a 5, 15, 30 and 45 s. The tip of the laser fibre was ofE.coliwasheatedupto50 Cinawaterhisnated lcohol before use. The laser ti

29、p was placed therewas only a minimal loss of viability of E.coli.Itwascon- tthermalresponsebecauseofjustheatingthethe Eppendorff tube and small horizontal s made in the direction of the bottom during the four fluid is not a sufficient explanation for the killing ure times. Laser settings by the manu

30、facturer (on a scale er 6 W 112 W), E. coli. Therefore, othsesmaybeinvolvedduringexpo- sure to the Nd:YAG laser (18). However, using a 50 Hz, Pulse duration 250 ls. The water and air coolant, ical for the Genius laser, was not activated during this inviroinwhichagarplates(inoculated with were ed to

31、laser light, temperatures close to 100 C cedure to avoid dilution of the dissolved anisms. measured immedia y after radiation. This t compensate for the lack of water and air coolant, tubes were kept on ice during the experiment. After expo- sure to the laser, the solution was extracted from the tub

32、es and microbial plating was performed (Fig. 1). Solutions were plated on blood agar plates to test for survival of the specific periodontal pathogens. For negative controls, the same proce- dure was performed without activating the laser (0 s).antimicrobial effect within this particular is caused b

33、y a photo-thermal effect n by photo-effectsof the laser light itself a ggested by Gro nquist etal. (20). Meral et al. (19) evaluated the minimal energy levels ne sarytohaveabactericidaleffectonabacterialsuspen variousoralanisms.Insteadofsubmergingthe, they observed a distance of 10 mm. Within labora

34、tory setting, they t the addition of a (e.g.sheepblood)enhancedtheabsorptionofthelaserThe efficacy of the Nd:YAG lasers has also been for the photo-thermal disinfection of root s by Bergmans et al. (21). Although a different indication, Table 2 shows the presence or absence of bacterial growth speci

35、fic microbiological species on the culture plates in invir s used bare resemblance to the present gation. Laser irradiation was performed in wet root stto ure time of the Nd:YAG laser. As a control bacterial of hadbeeninoculatedthe baseline as well as ure to -activated faecalis(a relatively heat-res

36、istant bacterium). The laser appli- cation was found to be safe and to have a potential disinfection capacity (99.7% kill). In a comparable study by Wang et al. (21), the Er,Cr:YSGG laser was compared with the Nd:YAG laser. Both laser systems had a significant bactericidal effect on infected root c

37、s, but the Nd:YAG was more effective resulting in 98% kill. In a more basic in viroendodontic m , laser radiation was delivered to 12 ll E.faecalislaser (0 s) showed confluent growth on all culture plates of test bacteria (Fig. 2a and b). After 5 s of ure, a otal colony forming units of all test spe

38、cies was (Fig. 2c). After ure of 15 s, no colony forming units observed for any of the six selected microbiological Consequently at 30 and 45 s of ure, for all the six odontalpathogens,thecultureplatesweresterile(Fig.cultures in small capillary tubes. For t received total energy of 60 J, no acteria

39、were detected ing laser treatment (Rooney et al. The most frequently used method to reduce the numbers pu iveperiodontalpathogensfromtheperiodontalpocketmechanical debridement of the root . This can be JDentHygiene8,2010;Kranendonk et al. Bactericidal effect of a Nd:YAG Fig. 2. (a) Total colony form

40、ing units of Prevo laermediaatbaseline;(b)0sof ure; (c) 5 s of exure; (d) 15 s ofhe present experiment, energy settings were used at 2 Badersten A, Nilveus R, Egelberg J. Effect of -surgical peri- odontal therapy, II. Severely advanced periodontitis. J Clin Period- ontol 1984; 11: 6376.level as a cl

41、ear dose response n percentage of heliterature (7 he effective- ness teria killed andKranendonk et al. Bactericidal effect of a Nd:YAG Fig. 2. (a) Total colony forming units of Prevo laermediaatbaseline;(b)0sof ure; (c) 5 s of exure; (d) 15 s ofhe present experiment, energy settings were used at 2 B

42、adersten A, Nilveus R, Egelberg J. Effect of -surgical peri- odontal therapy, II. Severely advanced periodontitis. J Clin Period- ontol 1984; 11: 6376.level as a clear dose response n percentage of heliterature (7 he effective- ness teria killed and energy density used is 3 Mombelli A, id B, Rutar A

43、, Lang N. Persistence patterns 9). in contact time might Porphyromonas gingivalis, ens,anditans after mechanical therapy of eliminating periodontal pathogens as the degree of disease.JPeriodontol2000;71:and thereby sible elimination of peri- odontal 4 Cobb CM, McCawley TK, Killoy WJ. A preliminary s

44、tudy on takes he Eppendorff tubes. This elimination of effects of the Nd:YAG laser on root and subgingival flora in vivo. JPeriodontol1992; 63: 5 Ando Y, Aoki A, Watanabe H, Ishikawa I. Bactericidal effect is proportional to the amount of energy absorbed by the Energy is a product time); therefore,

45、it er and duration of si- ble to alter either the ure erbium YAG laser on periodontal bacteria. 19:6 Morlock BJ, Pippin DJ, Cobb CM, Killoy W, effect of Nd:YAG laser exure on root surfarg Med the depth er or duration of by Rapley JW. The when used as the thereby increas- the adjunct to root planning

46、: an in vitro study. J Periodontol 1992; , the experiment revealed the usefulness the Nd:YAG laser in a7 Dobson J, Wilson M. Sensitization of oral bacteria in bio function of ure killing by light from a er laser. Arch Oral Biol 1992; Iwase T, Saito T, Nara Y, Morioka T. Inhibitory effect of HeNe las

47、er on dental plaque de ition in hamsters. J Periodontal Res 1989; 24: 282283.Wilson M, Dobson J, Sarkar S. Sensitization of periodontopathogen- ic bacteria to killing by light from a low- er laser. Oral Microbiol Immunol 1993; 8: 182187.Wilson M. Bactericidal effect of laser light and its potential

48、use in the treatment of plaque-related diseases. Dent J 1994; 44: 181 As contemporary treatment aims at a reduction of supra and subgingival bacterial mass by and planing the root , the Nd:YAG laser might be usefuladjuncttothistreatmentineliminating11 Ben Hatit Y, Blum R, Severin C, , Jabro MH. his

49、in vitro effects of a pulsed Nd:YAG laser on subgingival bacterial flora and on cementum: an in vivo study. J Clin Laser Med Surg 1996; 14: 12 Rooney J, Midda M, Leeming J. A laboratory investigation of the bactericidal effect of a NdYAG laser. BrDentJ1994; 176: 61 , 15 s of Nd:YAG laser use was fou

50、nd be 13 Slots J. Selective medium for isolation of -1 Axelsson P, Lindhe itans.Jicrobiol1982;4:The significance of ma enance care in treatment of periodontal disease. J Clin Periodontol 1981; 8: 281 JDentHygiene8,2010;Kranendonk et al. Bactericidal effect of a Nd:YAG 14 Van Steenbergen MTJ, Van J,

51、Van der Mispel L, 18Kranendonk et al. Bactericidal effect of a Nd:YAG 14 Van Steenbergen MTJ, Van J, Van der Mispel L, 18 Bergmans L, Moisiadis P, Teughels W, Van meerbeek B, Quirynen M, Lambrechts P. Bactericidal effect of Nd:YAG laser irradiatioder Velden U, Abbas F, De Graaff J. Comparison of two

52、 mediafor24:itans. Jicrobiol someendodonticpathogensexEndodJ2006;39:19 Meral G, Tasar F, Kocago z S, Sener C. Factors affecting the Ward GD, Watson IA, Stewart-Tull DE et al. Bactericidal action of er Nd:YAG laser light on Escherichiacoliin saline suspen-.JAppl Microbiol 2000; 89: Lioubavina N, Jens

53、en J, Karring T. Evaluation of periodontal bacterial effects of Nd:YAG laser in vivo. rg Med 2003; 20 Gro nquist A, Wistro m J, Axner O, Monsen TJ. Bactericidal effect of pulsed 1,064 nm Nd:YAG laser light on Staphylococcus dermis is of photo-thermal origin. Laser Surg Med 2000; 27: 336340.ets with

54、Nd:YAG laser. J DentRes 1997; 77: (Spe no. 19258.17 Raffetto N. Lasers for initial periodontal therapy. Dent Clin North Am 2004; 48: 923936.l e) b-21 , Zhang C-f, Yin X-z. Evaluation of the of Er,Cr:YSGG, and Nd:YAG lasers in infected root s. J Endod 2007; 33: JDentHygiene8,2010;2014272the bodys nut

55、itionalake.Theelderlyusuallyhavealotof nity ability decreased. Nowadays，the periodontal NdYAG激光非手术疗法在老chbgingivalscalingandrootplanning牙surgicaltreatment，drugtreatment，alwayshavesomecies，when used in elderly periodontitis patients. Considering theelderlytoleranceabilityofcomplextreatment，we向学熔，晏 莺，杨

56、 梅-surgicalperiodontaltreatment.AtpresenttheYAG laser has been widely used in periodontal therapy. articlewillreportoncharacteristicsof2014272the bodys nutitionalake.Theelderlyusuallyhavealotof nity ability decreased. Nowadays，the periodontal NdYAG激光非手术疗法在老chbgingivalscalingandrootplanning牙surgicalt

57、reatment，drugtreatment，alwayshavesomecies，when used in elderly periodontitis patients. Considering theelderlytoleranceabilityofcomplextreatment，we向学熔，晏 莺，杨 梅-surgicalperiodontaltreatment.AtpresenttheYAG laser has been widely used in periodontal therapy. articlewillreportoncharacteristicsoftheelderly

58、-surgicalperiodontaltreatmenttechnologies，and士advantages of NdYAG he periodontalastoprovide theoreticalbasisforNdYAG lasertreatmentperiodontitisinelderlyment；NdYAG 学余篇近五年来负责参与各级科研项目十余项10 年很大差距。20世纪末，牙周病与全身健康和疾病联系起来。慢性是人类疾病中普遍存在 治疗技术、NdYAG 激光在牙周治疗中的优越性等方面老年非手术治疗；NdYAG 激The application prospect -surgi

59、cal treatmentn NdYAGlaserfor theelderly 根据病情发展调整方案1。选择一种既能ated Hospital of Stomatology，Chongqing ety，how to improve life equality of the elderly is a problem us.Chronicperiodontitisissoheelderly.Itonlyaffectschewing，butalsoaffectsthedigestion，espe 1 1.牙电子Journal ofPractical Feb.2014Vol.7No.效和牙如2.的第六

60、并发症2Humphrey等 发现，牙周病是冠心病的独，2.药物治药物治疗只能作为基础治疗等4口腔菌群不良口致1.Journal ofPractical Feb.2014Vol.7No.效和牙如2.的第六并发症2Humphrey等 发现，牙周病是冠心病的独，2.药物治药物治疗只能作为基础治疗等4口腔菌群不良口致1.牙周特老年患者病情一般较重部一般都是遵循循证医学原则有针对性用药，合理和家的庭2.合治合治疗是指通过调系。研究在消除分布均牙合关系协牙齿和牙周组织得到均匀的功能性外力刺激有利牙周组织2. 4 松牙固定术制此时动度较大的牙齿已很难恢复但是不处2 牙20142723. 2 NdYAG 激光