SDS-PAGE浓缩胶配方表

1、抗生素的贮存溶液及其工作浓度*1以水为溶剂的抗生素贮存液应通过0.22 pim滤器过滤除菌。以乙醇为溶剂的抗生素溶液无须除菌处理。所有抗生素溶液均应保存于不透光的容器中。抗生素贮存液M工作浓度浓度保存条件严紧型质粒松弛型质粒氨羊青霉素50 mg ml （溶于水）-20 C20 ml60 %ig ml樱莘青石素50 mgmI （溶于水）-20C20 pg ml60 间 ml氯霉素34 mgmI （溶于乙好）-20 C25 ml170 jig ml卡那霉素10mg ml （溶于水）-20 C10 pg ml50 pg ml链霉素10 mg ml （溶于水）-20C10 pg ml50 j.ig m

2、l四环素g5mgmI （溶于乙睥）-20C10 Ng ml50 pg ml*2镁离子是四环素的拮抗剂，四环素抗性菌的筛选应使用不含镁盐的培养基（如LB培养基）。SDS浓缩胶(5% Acrylamide)配方表各种组份名称HzO30% Acrylamide1.0MTris-HCI (pH6.8) 10% SDS10%过磕酸铁TEMEDof0.010.010.001各种凝胶体积所对应的各种组份的取样显m42 10.02黑0.030.030.0035H345O.0 040.040.0040 050.050.0050.060.060.0060.080.080.0080.10.10.01SDS分离胶配方

3、表各种组份名称各种凝胶体积所对应的各种组份的取样量5 ml 10 ml 15 ml 20 ml 25 ml 30 ml 40 ml 50 ml6% GelH2O30o Acrylamide1 5 M Tris-HCI (pH8 8)10o SDS10%过硫酸铉TEMED8% GelFLO30o Acrylamide1 5 M Tris-HCI (pH8 8)10o SDS10o过硫酸镣TEMED10% GelH2O30% Acrylamide1.5 M Tris-HCI (pH8.8)10o SDS10o过硫酸钱TEMED12% GelHsO30o Acrylamide1.5 M Tris-H

4、CI (pH8.8)10o SDS10o过硫酸玻TEMED15% GelH2O30o Acrylamide1.5 M Tris-HCI (pH8.8)10o SDS10%过硫酸核TEMED2.65.37.910.613.215.921.226.51 02.03.04.05.06.08 010.0132.53.85.06.37.510 012.50 050.10.150.20.250.30.40.50 050.10.150.20.250.30 40.50.0040.0080.0120.0160.020.0240.0320.042.34.66.99.311.513.918.523.21.32.74

5、.05.36.78010.713.31 32.53.85.06.37.510.012.50.050.10.150.20.250.30.40.50.050.10.150.20.250.30.40.50.0030.0060.0090.0120.0150.0180.0240.031.94.0173.31.32.50.050.10.050.15.97.95.06.73.85.00.150.20.150.29 3 3252539 86.。o。11 915919.810.013 316.77.510.012.50.30.40.50.30.40.50.0120.0160.02163.34.96.68.29913216.52 04.06.08.010.012016020.01.32.53.85.06.37.510.012.50.050.10.150.20.250.30.40.50.050.10.150.20.250.30.40.50 0020.0040.0060.0080.010 0120 0160.021.12.33.44.65.7699211.52.55.07.510.012.515.020.025.01.32.53.85.06.37.510.012.50