Ruthenium-red-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•Agonists•ScreeningLibrariesRutheniumredCat.No.:HY-103311CASNo.:11103-72-3作⽤靶点:CalciumChannel作⽤通路:MembraneTransporter/IonChannel;NeuronalSignaling储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体DMSO:10mg/mL(Needultrasonic)外H2O:<0.1mg/mL(ultrasonic)(insoluble)实请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制验储备液，并请注意储备液的保存⽅式和期限。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InV制澄的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶1.请依序添加每种溶剂：10%DMSO90%(20%SBE-β-CDinsaline)Solubility:1mg/mL(InfinitymM);Clearsolution;Needultrasonic此⽅案可获得1mg/mL(InfinitymM)的澄溶液。以1mL⼯作液为例，取100μL10.0mg/mL的澄DMSO储备液加到900μL20%的⽣理盐⽔⽔溶液中，混合均匀。BIOLOGICALACTIVITY⽣物活性Rutheniumred(Ammoniatedrutheniumoxychloride)⼀种聚阳离⼦染料，⼴泛⽤于电⼦显微镜(EM)下细胞、组织和营养细的观察。Rutheniumred能与磷脂和脂酸反应强烈，并与酸性粘多糖结合。Rutheniumred还⼀种L型钙电流(ICa)阻断剂[1][2]。IC50&TargetICa[2]1/2体外研究Rutheniumredstainingofintracellularorganellesandstructuresseemedtodependonthetimeofexposuretthisagent,aswellontheconcentrationused[2].RutheniumredefficientlyblockstheL-typecalciumcurrentinadose-dependentmannerininguinea-pigisolatedventricularheartcells,withEC50of0.8μM[2].Rutheniumred(10μM)blocksthesarcoplasmicCareleasechannelsorthemitochondrialCauptake,andblocks26.7%ofthesodiumcurrent,andsloweditsinactivationtime-courseinguinea-pigisolatedventricularheartcells[2].REFERENCES[1].LashandaNWaller,etal.RutheniumredstainingforultrastructuralvisualizationofaglycoproteinlayersurroundingthesporeofBacilluanthracisandBacillussubtilis.JMicrobiolMethods.2004Jul;58(1):23-30.[2].ClaireOMalécot,etal.Rutheniumredasaneffectiveblockerofcalciumandsodiumcurrentsinguinea-pigisolatedventricularheartcells.BrJPharmacol.1998Jun;124(3):465–472.McePdfHeightMedChemExpress(MCE)只为有资质的科研机构、医药企业基于科学研究或药证申报的⽤途