Oenothein-B-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEOenotheinBCat.No.:HY-N7765CASNo.:104987-36-2分⼦式:C₆₈H₅₀O₄₄分⼦量:1571.1作⽤靶点:HCV;Bacterial;Apoptosis;Fungal作⽤通路:Anti-infection;Apoptosis储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性OenotheinB⼀种⼆聚体⼤环鞣花单宁，具有⼴泛的药理活性，包括抗氧化、抗炎、抗真、抗HCV和抗肿瘤特性。OenotheinB⼀种有效且特异性的聚(ADP-核糖)糖⽔解酶抑制剂。体外研究OenotheinB(15-45µM;12-72hours)hasadose-andtime-dependentinhibitionrateeffectonA549cellsintherangeof12hours,24hours,36hours,48hours,60hoursand72hoursofexposure[1].OenotheinB(15-45µM;24hours)effectivelyinhibitstheproliferationofA549cellsbyinducingapoptosisandarrestingcellsatG1stage[1].OenotheinB(15-45µM;24hours)notonlyincreasesthelevelofintracellularreactiveoxygenspecies(ROS),butalsoinducestheupregulationofintracellularapoptotictriggers(cleavagecaspase-3,PARP,cytochromeclevelinthecytosol,Bax)[1].A549cells[1]15µM,30µMand45µM24hoursArrestedcellsintheG1phase.A549cells[1]15µM,30µMand45µM24hoursBAX,p53,cytochromec(cytoplasm)andPARPwereunregulatedsignificantly;Anti-apoptoticBcl-2wasdecreasedsignificantlyinaconcentration-dependentmanner.CellViabilityAssay[1]CellLine:A549cellsConcentration:15µM,30µMand45µMIncubationTime:12hours,24hours,36hours,48hours,60hoursand72hoursResult:EffectivelyinhibitedtheproliferationofA549cells.ApoptosisAnalysis[1]1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemECellLine:A549cellsConcentration:15µM,30µMand45µMIncubationTime:24hoursResult:InducedapoptosisinA549cells.CellCycleAnalysis[1]CellLine:A549cellsConcentration:15µM,30µMand45µMIncubationTime:24hoursResult:ArrestedcellsintheG1phase.WesternBlotAnalysis[1]CellLine:A549cellsConcentration:15µM,30µMand45µMIncubationTime:24hoursResult:BAX,p53,cytochromec(cytoplasm)andPARPwereunregulatedsignificantly;Anti-apoptoticBcl-2wasdecreasedsignificantlyinaconcentration-dependentmanner.体内研究OenotheinB(100-300mg/kg;p.o.)hastheabilitytoreduceneuroinflammationinthebrainduringsystemicinflammationinICRmice[3].REFERENCES[1].XiaodongPei,etal.OenotheinBinhibitshumannon-smallcelllungcancerA549cellproliferationbyROS-mediatedPI3K/Akt/NF-κBsignalingpathway.ChemBiolInteract.2019Jan25;298:112-120.[2].SatoruTamura,etal.OenotheinB,dimerichydrolysabletannininhibitingHCVinvasionfromOenotheraerythrosepala.JNatMed.2019Jan;73(1):67-75.[3].SatoshiOkuyama,etal.OenotheinBsuppresseslipopolysaccharide(LPS)-inducedinflammationinthemousebrain.IntJMolSci.2013May7;14(5):9767-78.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedical