BDFACSCalibur流式细胞仪培训手册

格+式编编辑格+式编编辑FACS101Handbook-FACS101Handbook-—--WORD格式一可编辑表一、常用荧光染剂测量参数荧光染剂吸光波长(nm)荧光波长(nm)标示抗体用染剂Fluorescein,FITC490520Phycoerythrin-R,PE495578Peridinin-chlorophyll,PerCP490677Cy-Chrome495670PE-TexasRed495620PE-Cyanine5495670核酸含量分析EthidiumBromide510(+dsDNA)595PropidiumIodide,PI536(+dsDNA)623AcridineOrange480(+DNA)440-70(+RNA)520650ThiazoleOrange509(+RNA)533PyronineY560-562(+dsRNA)497(+ssRNA)565-574563细胞ViabilityPropidiumIodide,PI536623YOPRO-1480515AcridineOrange4805207-AAD488670细胞膜电位DiO-C6(3)485510Mitochondrial膜电位Rhodamine123485546细胞内pH值BCECF-AM488Ratio520/620SNARF1-AM514Ratio587/640细胞内钙浓度Fluo4-AM488528CalciumGreen-1488530FuraRed488660H2O2sensitiveDihydrorhodamine123505534DCFH-DA505535O2-radicalsensitiveHydroethidine505600EsterasesensitiveFluorescein-DA495525

表二、典型的仪器设定SingleColorTwoColorThreeColorFITCPEFITC/PEFITC/PE/PerCPThresholdFSCFSCFSCFSCValue52525252FSCVoltageE00(Lin)E00(Lin)E00(Lin)E00(Lin)SSCVoltage350(Lin)350(Lin)350(Lin)350(Lin)FL1Voltage600(Log)-600(Log)600(Log)FL2Voltage-540(Log)540(Log)540(Log)FL3Voltage-650(Log)FL1-%FL20.0%0.0%0.7%0.7%FL2-%FL10.0%0.0%25.0%25.0%FL2-%FL30.0%0.0%0.0%0.0%FL3-%FL20.0%0.0%0.0%17.0%表三、需定期更换耗材需定期更换耗材更换时机BD目录号BDFalcon352052试管上机专用试管。352052(泛泰公司0800213029)BDFalcon352235试管附滤网FALCON试管，上机前去除样品中之细胞团块，防止管路堵塞。352235(泛泰公司0800213029)BDFACSFlow上机用平衡缓冲液。体积20公升。342003(腾达行02-27202215)BDFACSClean专为细胞仪日常清洁设计。体积5公升。340345(腾达行02-27202215)BDFACSRinse专为细胞仪日常除污设计。体积5公升。340346(腾达行02