NT1-O12B-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemENT1-O12BCat.No.:HY-137499分⼦式:C₃₆H₆₀N₂O₄S₄分⼦量:713.13作⽤靶点:EndogenousMetabolite作⽤通路:MetabolicEnzyme/Protease储存⽅式:Pureform-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:200mg/mL(280.45mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.4023mL7.0113mL14.0227mL5mM0.2805mL1.4023mL2.8045mL10mM0.1402mL0.7011mL1.4023mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥5mg/mL(7.01mM);Suspendedsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:5mg/mL(7.01mM);Suspendedsolution;Needultrasonic1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥5mg/mL(7.01mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性NT1-O12B⼀种内性化学物质，也⼀种神经递质衍⽣的类脂质体(NT-lipidoid)，⼏种⾎脑屏障(BBB)⾮渗透性物质增强脑内转运的有效载体。将NT1-O12B掺杂到BBB⾮渗透性脂质纳⽶粒(LNPs)中，可以使LNPs具有穿过BBB的能⼒。NT类脂质体不仅有利于BBB的跨膜转运，⽽且可以将其转运到神经细胞中进⾏功能因沉默或因重组。体外研究NT1-O12Bindicatesalipidoidcontainingatryptamineheadgroupandahydrophobictailgroupcontaining12carbonatoms[1].体内研究ItencapsulatesAmBinpureNT1-lipidoids(namelyNT1-O12B,NT1-O14B,NT1-O16B,andNT1-O18B)usingaproceduresimilartotheDiRencapsulation.NT1-O12BisthedopantforenhancedbraindeliverybecauseitshowsthehighestDiRfluorescenceintensityamongallNT-lipidoids.DopingNT1-O12BtotheBBB-impermeablelipidoidPBA-Q76-O16BresultedinanAmBformulationthatcouldcrossBBB.Usingthisapproach,theAmBconcentrationinthebraintissuereachedashighas300ng/g(AmB/tissue)withadeliveryefficiencyofabout0.135%ofinjecteddoseafter24hoursofintravenousinjectionwithAmB(5mg/kg)[1].REFERENCES[1].MaF,etal.Neurotransmitter-derivedlipidoids(NT-lipidoids)forenhancedbraindeliverythroughintravenousinjection.SciAdv.2020;6(30):eabb4429.Published2020Jul24.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearc