DNA固定在纳米颗粒表面的方法

磷酸化的DNABiotin-DNA炔基修饰的DNA巯基修饰的DNA氨基修饰的DNA金银纳米颗粒碳纳米管、石墨烯二氧化硅纳米颗粒量子点上转换纳米颗粒磷酸化的DNA与TiO2π-π堆积，如单链DNA与石墨烯，碳纳米管静电吸附1、Biotin-DNA2、炔基修饰的DNA及其他生物分子3、NH2-DNA氨基羧基氨基羧基氨基氨基1〕EDC和NHSForbestresultsintwo-stepreactions,performthefirstreactioninMESbuffer(orothernon-amine,non-carboxylatebuffer)atpH5-6,thenraisethepHto7.2-7.5withphosphatebuffer(orothernon-aminebuffer)immediatelybeforereactiontotheamine-containingmolecule氨基羧基DCC〔N,N′-Dicyclohexylcarbodiimide〕氨基-氨基1〕BS(PEG)nPEGCrosslinkers-AmineReactive2〕TSAT(Tris-succinimidylaminotriacetate)WaterWaterNHSesters:pH7-93〕DSS(disuccinimidylsuberate)4〕BSOCOES(bis[2-(succinimidyloxycarbonyloxy)ethyl]sulfone)5〕DSG(disuccinimidylglutarate)6〕DTSSP(3,3´-Dithiobis[sulfosuccinimidylpropionate])WaterWaterWaterDMSOorDMF8〕BS³(Bis[sulfosuccinimidyl]suberate)9〕EGS[ethyleneglycolbis(succinimidylsuccinate)]

10〕Sulfo-EGS[ethyleneglycolbis(sulfosuccinimidylsuccinate)]7〕DST(disuccinimidyltartrate)WaterWaterDMSOorDMFDMSOorDMF4、巯基化的DNA1Au-SAg-S2巯基氨基3巯基羧基4巯基羟基5巯基巯基巯基-氨基1〕AMAS(N-[α-maleimidoacetoxy]succinimideester)NHSesters:pH7-9；；。TwostepsreactionDMSOorDMF2〕BMPS(N-[β-maleimidopropyloxy]succinimideester)DMSOorDMF3〕EMCS(N-[ε-maleimidocaproyloxy]succinimideester)DMSOorDMF4〕Sulfo-EMCS(N-[ε-maleimidocaproyloxy]sulfosuccinimideester)Water6〕Sulfo-GMBS(N-[γ-maleimidobutyryloxy]sulfosuccinimideester)5〕GMBS(N-[γ-maleimidobutyryloxy]succinimideester)DMSOorDMFWater7〕BMPA(N-β-maleimidopropionicacid)EDC，8〕LC-SMCC(Succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxy-[6-amidocaproate])DMSOorDMF10〕Sulfo-SMPB(sulfosuccinimidyl4-[p-maleimidophenyl]butyrate)9〕SMPB(succinimidyl4-[p-maleimidophenyl]butyrate)DMSOorDMFWater11〕Sulfo-LC-SPDP,LC-SPDP,SPDP巯基-羧基巯基-羟基异氰酸脂在水溶液中很容易水解，所以最好用有机溶剂如DMSO、DMF等。如在水相中，那么要使用pH8.5的碱性缓冲溶液，防止使用含羟基的缓冲液如tris缓冲液，且要注意含羟基的分子不能有氨基和巯基，因为pH＞7.5顺丁烯二酰亚胺失去对巯基的特异性，会