GW-441756-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEGW441756Cat.No.:HY-18314CASNo.:504433-23-2分⼦式:C₁₇H₁₃N₃O分⼦量:275.3作⽤靶点:TrkReceptor;Apoptosis作⽤通路:NeuronalSignaling;ProteinTyrosineKinase/RTK;Apoptosis储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:17.5mg/mL(63.57mM;Needultrasonicandwarming)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.6324mL18.1620mL36.3240mL5mM0.7265mL3.6324mL7.2648mL10mM0.3632mL1.8162mL3.6324mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(7.56mM);Clearsolution1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEBIOLOGICALACTIVITY⽣物活性GW441756⼀种⾼效特异性神经⽣长因⼦(NGF)受体酪氨酸激酶a(TrkA)抑制剂(IC50=2nM)，可消除BmKNSPK-induced神经突⽣长[1][2][3]。IC50&TargetTrkA2nM(IC50)体外研究GW-441756(1µM;48hours;spinalcordneuronscells)abolishsBmKNSPK-inducedneuriteoutgrowth[1].GW-441756(1µM;4hours;PC12cells)inhibitsnervegrowthfactorinducedneuriteoutgrowth[1].Immunofluorescence[1]CellLine:SpinalcordneuronscellsConcentration:1µMIncubationTime:48hoursResult:AbolishedBmKNSPK-inducedneuriteoutgrowth.户使⽤本产品发表的科研⽂献•CellMetab.2022Nov11;S1550-4131(22)00490-9.•JHazardMater.2022Jan15;422:126850.•JFunctFoods.2018Jul;46:175-184.•bioRxiv.2021Feb17.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].ZhaoF,etal.BmKNSPK,aPotentPotassiumChannelInhibitorfromScorpionButhusmartensiiKarsch,PromotesNeuriteOutgrowthviaNGF/TrkASignalingPathway.Toxins(Basel).2021;13(1):33.Published2021Jan5.[2].TeradaK,etal.Cholinesteraseinhibitorrivastigmineenhancesnervegrowthfactor-inducedneuriteoutgrowthinPC12cellsviasigma-1andsigma-2receptors.PLoSOne.2018;13(12):e0209250.Published2018Dec17.[3].WoodER,etal.DiscoveryandinvitroevaluationofpotentTrkAkinaseinhibitors:oxindoleandaza-oxindoles.BioorgMedChemLett.2004;14(4):953-957.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresea