Congo-Red-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemECongoRedCat.No.:HY-D0236CASNo.:573-58-0分⼦式:C₃₂H₂₂N₆Na₂O₆S₂分⼦量:696.66作⽤靶点:Others作⽤通路:Others储存⽅式:4°C,sealedstorage,awayfrommoistureandlight*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoistureandlight)溶解性数据体外实验DMSO:20mg/mL(28.71mM;Needultrasonic)H2O:<0.1mg/mL(insoluble)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.4354mL7.1771mL14.3542mL5mM0.2871mL1.4354mL2.8708mL10mM0.1435mL0.7177mL1.4354mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoistureandlight)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2mg/mL(2.87mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥2mg/mL(2.87mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性CongoRed⼀种偶氮染料。CongoRed结合已被⼴泛⽤于组织切⽚中淀粉样蛋⽩的测定。体外研究CongoRedhistochemicalstainmayserveasasimplescreeningtoolforinvestigatingiftheaggregatesinmutantcellshavemisfoldedβ-pleatedsheetsecondarystructures.CongoRedhistochemicaldyehastheabilitytobindspecificallytocrossedβ-pleatedsheetstructures.Wild-typeHSPB1shouldmaintainproteinhomeostasisbybindingproteinsinnon-nativeconformations,therebypreventingsubstrateaggregation.TheT139Mmutant,however,failsinthisfunctionandresultsinanaccumulationofmisfoldedproteins,whicharetargetedbyCongoRedforintercalatingbetweentheβ-pleatedsheetstructures.CongoRedhistochemicalstainmayserveasasimpletooltoinvestigateiftheaggregatesinmutantcellshavemisfoldedβ-pleatedsheetsecondarystructures[1].PROTOCOLCellAssay[1]HeLacellsareselectedforthesestudiesduetotheirlargecytoplasmicvolume.Cellstransfectedwithmutantorwild-typeHSPB1constructsaregrownoncoverslipsfor24hrandthenarestainedwithCongoredtodetermineiftheaggregatesdisplayamyloidogenicproperties.Briefly,cellsarefirstfixedwith10%formalinfor10minandstainedwith1%Congoredfor5min,followedbydestainingwith0.01%potassiumhydroxidein50%ethanol.Coverslipsarethenpassedthroughgradedethanolconcentrationsfordehydrationandmountedinamountingmediumandexaminedbyfluorescentmicroscopyunderrhodaminefilter[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•EurJPharmacol.2022Dec2;175446.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].AmornvitJ,etal.Anovelp.T139MmutationinHSPB1highlightingthephenotypicspectruminafamily.BrainBehav.2017Jul21;7(8):e00774.McePdfHeightCaution:Producthasnotbeenfullyvalidated