Deoxycholic-acid-Cholanoic-Acid-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEDeoxycholicacidCat.No.:HY-N0593CASNo.:83-44-3Synonyms:CholanoicAcid;Desoxycholicacid分⼦式:C₂₄H₄₀O₄分⼦量:392.57作⽤靶点:Gprotein-coupledBileAcidReceptor1;EndogenousMetabolite作⽤通路:GPCR/GProtein;MetabolicEnzyme/Protease储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:≥100mg/mL(254.73mM)H2O:<0.1mg/mL(ultrasonic;warming;heatto80°C)(insoluble)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM2.5473mL12.7366mL25.4732mL5mM0.5095mL2.5473mL5.0946mL10mM0.2547mL1.2737mL2.5473mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:2.5mg/mL(6.37mM);Suspendedsolution;Needultrasonic2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(6.37mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(6.37mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性Deoxycholicacid(cholanoicacid)⼀种胆汁酸，肠道代谢副产物，可以激活G蛋⽩偶联胆汁酸受体TGR5。IC50&TargetMicrobialMetaboliteHumanEndogenousMetabolite体外研究Deoxycholicacid(DCA)(100μM)inducestheproductionofgastriccancercelllineMGC803resistanttoacidifiedbileacidsandenhancestheirsurvivalandproliferationactivityunderbileacidstress[2].Deoxycholicacid(DCA)(100μM)-inducedresistantcellsshowsalteredmorphology,significantlyelevatedtelomeraseactivity,bettercellviabilityandreducesapoptosiscomparedtonormalMGC803cells[2].PROTOCOLCellAssay[2]MGC803cellsareculturedinRoswellParkMemorialInstitutemediasupplementedwith10%fetalcalfserumand100U/mLPenicillinand100mg/mLStreptomycin.TogenerateMGC803-resistantcells,thepHvalueoftheMGC803culturemediumisadjustedtotheexperimentalconditionsusingthehydrochloricacid(A).ThebileacidsGCDAandDeoxycholicacidaredilutedtooptimalworkingconcentrationsof100μM(B)withculturemedium,andtheoverallpH(A+B)isadjustedtopH5.5,simulatingthegastricenvironment.Initially,MGC803cellsarechronicallyexposedtoacidifiedmediumwithbileacids(A+B)for10minevery24h.Theexperimentaltimeandconditionsareoptimizedinourpreliminaryexperiments,whichshowthat10minisenoughanddoesnotresultincelldamage.Thisprocedureisrepeatedandittakes60weeksfortheMGC803cellstosurviveandproliferateundertheexposureofA+Bfor120min.ControluntreatedcellsareculturedinneutralRPMImediumatpH7.4inparalleltotheresistantcellsfor60weeks.ThemorphologicalchangesinMGC803cellsexposedtoacidifiedbileacids(A+B)aredocumentedat30and60weeks[2].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•CellRes.2019Mar;29(3):193-205.•NatCellBiol.2018Oct;20(10):1145-1158.•Microbiome.2019Mar20;7(1):43.•JInvestDermatol.19November2021.•CellDeathDiscov.2020Jul6;6:56.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESeemorecustomervalidationsonwww.MedChemEREFERENCES[1].SommE,etal.β-Klothodeficiencyprotectsagainstobesitythroughacrosstalkbetweenliver,microbiota,andbrownadiposetissue.JCIInsight.2017Apr20;2(8).pii:91809.[2].WangX,etal.Acidifiedbileacidsenhancetumorprogressionandtelomeraseactivityofgastriccancerinmicedependentonc-Mycexpression.CancerMed.2017Apr;6(4):788-797.McePdfHeightCau