Hydrocortisone-Cortisol-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEHydrocortisoneCat.No.:HY-N0583CASNo.:50-23-7Synonyms:Cortisol分⼦式:C₂₁H₃₀O₅分⼦量:362.46作⽤靶点:GlucocorticoidReceptor;EndogenousMetabolite作⽤通路:Immunology/Inflammation;MetabolicEnzyme/Protease储存⽅式:4°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light)溶解性数据体外实验DMSO:≥31mg/mL(85.53mM)H2O:<0.1mg/mL(insoluble)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM2.7589mL13.7946mL27.5893mL5mM0.5518mL2.7589mL5.5179mL10mM0.2759mL1.3795mL2.7589mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(protectfromlight)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(6.90mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(5.74mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(5.74mM);Clearsolution4.请依序添加每种溶剂：5%DMSO>>40%PEG300>>5%Tween-80>>50%salineSolubility:≥2.5mg/mL(6.90mM);Clearsolution5.请依序添加每种溶剂：5%DMSO>>95%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(6.90mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性Hydrocortisone(Cortisol)肾上腺⽪质分泌的类醇激素或糖⽪质激素[1]。IC50&TargetHumanEndogenousMetaboliteHumanEndogenousMetabolite体外研究Hydrocortisone(50nM)showsadose-dependentdown-regulationofGRtranscriptinhCMEC/D3cells.Hydrocortisonesupplementationoftheserum-reducedcelldifferentiationmediumleadstoasignificantincreaseinTERacrossthehCMEC/D3monolayer[1].Hydrocortisone-treatedDendriticcells(DCs)showadecreasedexpressionofMHCIImolecules,thecostimulatorymoleculeCD86,andtheDC-specificmarkerCD83,aswellasastronglyreducedIL-12secretion.Hydrocortisone-treatedDCsinhibitproductionofIFN-γbutinduceanincreasedreleaseofIL-4andnochangeinIL-5[2].Hydrocortisonereducespostischemicoxidativestress,perfusionpressure,andtransudateformation.Hydrocortisoneinhibitspostischemicsheddingofsyndecan-1,heparansulfate,andhyaluronanasisreleaseofhistaminefromresidentmastcells[3].PROTOCOLCellAssay[1]CellsareplatedontopofcollagenIV-coatedtranswellchambersforsix-wellplates(24mmdiameter,membranematerial:polyethyleneterephthalate(PET),0.4μmpores,poredensity1.6×106cm2)atdensitiesof2.5×104cellscm2perwell.Whentheyhavereachedconfluenceatday5,thedifferentexperimentalsetsofcellsaretransferredtodifferentiationmediumcontainingreducedamountsofFCSandtreatedwithTNFαorhydrocortisoneasindicated.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•Nature.2021Jan;589(7843):620-626.•NatChemBiol.2022Aug18.•CancerCommun(Lond).2021Jul;41(7):576-595.•JCIInsight.2021Jun22;6(12):142838.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE•OxidMedCellLongev.2022Jan27;2022:7502632.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].FörsterC,etal.DifferentialeffectsofhydrocortisoneandTNFalphaontightjunctionproteinsinaninvitromodelofthehumanblood-brainbarrier.JPhysiol.2008Apr1;586(7):1937-49.[2].BellinghausenI,etal.InhibitionofhumanallergicT-cellresponsesbyIL-10-treateddendriticcells:differencesfromhydrocortisone-treateddendriticcells.JAllergyClinImmunol.2001Aug;108(2):242-9.[3].ChappellD,etal.Hydrocortisonepreservesthevascularbarrierbyprotectingtheendothelialglycocalyx.Anesthesiology.2007Nov;107(5):776-84.McePdfHeigh