Hygromycin-B-Hygrovetine-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEHygromycinBCat.No.:HY-B0490CASNo.:31282-04-9Synonyms:Hygrovetine分⼦式:C₂₀H₃₇N₃O₁₃分⼦量:527.52作⽤靶点:Bacterial;Fungal;Antibiotic;Parasite作⽤通路:Anti-infection储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:100mg/mL(189.57mM;ultrasonicandwarmingandheatto60°C)H2O:50mg/mL(94.78mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.8957mL9.4783mL18.9566mL5mM0.3791mL1.8957mL3.7913mL10mM0.1896mL0.9478mL1.8957mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：1%DMSO>>99%salineSolubility:≥0.5mg/mL(0.95mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥5mg/mL(9.48mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥5mg/mL(9.48mM);Clearsolution4.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥5mg/mL(9.48mM);Clearsolution5.请依序添加每种溶剂：5%DMSO>>40%PEG300>>5%Tween-80>>50%salineSolubility:≥2.5mg/mL(4.74mM);Clearsolution6.请依序添加每种溶剂：5%DMSO>>95%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(4.74mM);Clearsolution7.请依序添加每种溶剂：PBSSolubility:100mg/mL(189.57mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY⽣物活性HygromycinB抑制原核和真核细胞的氨糖苷类抗⽣素。IC50&TargetAminoglycoside体外研究HygromycinB,anaminocyclitolantibioticthatstronglyinhibitsboth70Sand80Sribosomes,issynthesizedbyStreptomyceshygroscopicus[1].HygromycinBat0.38mMconcentrationcompletelyhaltsyeastcellgrowthinrichmedia,presumablybypreventingproteinsynthesisbycytoplasmicribosomes.Polypeptidesynthesisincell-freeextractsfromrabbitreticulocytes,wheatgermandyeastisstronglyblockedbylowconcentrationsofhygromycinB.TheantibioticinhibitspeptidechainelongationbyyeastpolysomesbypreventingelongationfactorEF-2-dependenttranslocation.TheinhibitionoftranslocationbyhygromycinBmightresultfromthestabilizationofpeptidyl-tRNAboundtotheribosomalacceptorsite[2].体内研究HygromycinBinhibitsproteinsynthesisbyblockingribosomaltranslocationwithoutcausingsignificantmisreadinginvivo[3].ConstitutiveexpressionofthebacterialhygRgeneintransgenicmiceinvivoconfersresistancetohygromycinB[4].PROTOCOLAnimalHygromycinBisdissolvedinsterilewater.ThemiceC57BL/6J-TgN(pPWL512hyg)1EmscarryinghygRareAdministration[4]treatedwithasingledoseofhygromycinBi.p.atdosesthatstartsat2.7mg/kgandincreasesby50%foreachconsecutivedose.Controlwild-typeC57BL/6Jmicearetreatedwiththesamevolumeofsterilesaline.Totalvolumeinjectedis0.5mL.Thehealthstatusandbodyweightsofanimalsaremonitoreddailyfor10consecutivedays[4].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE户使⽤本产品发表的科研⽂献•AntimicrobAgentsChemother.2022Nov10;e0097722.•AmJTranslRes.2021Apr15;13(4):2331-2349.•BiochemBiophysResCommun.6August2022.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].PardoJM,etal.BiochemicalbasisofresistancetohygromycinBinStreptomyceshygroscopicus--theproducingorganism.JGenMicrobiol.1985Jun;131(6):1289-98.[2].GonzálezA,etal.StudiesonthemodeofactionofhygromycinB,aninhibitoroftranslocationineukaryotes.BiochimBiophysActa.1978Dec21;521(2):459-69.[3].PfisterP,etal.Roleof16SrRNAHelix44inRibosomalResistancetoHygromycinB.AntimicrobAgentsChemother.2003May;47(5):1496-502.McePdfHei