Propidium-Iodide-PI-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEPropidiumIodideCat.No.:HY-D0815CASNo.:25535-16-4Synonyms:PI分⼦式:C₂₇H₃₄I₂N₄分⼦量:668.39作⽤靶点:Others作⽤通路:Others储存⽅式:4°C,sealedstorage,awayfrommoistureandlight*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoistureandlight)溶解性数据体外实验DMSO:100mg/mL(149.61mM;Needultrasonic)H2O:3.57mg/mL(5.34mM;ultrasonicandwarmingandheatto60°C)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.4961mL7.4807mL14.9613mL5mM0.2992mL1.4961mL2.9923mL10mM0.1496mL0.7481mL1.4961mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoistureandlight)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%saline1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥2.5mg/mL(3.74mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(3.74mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性PropidiumIodide可⽤于细胞染⾊的红⾊荧光染料。体外研究PropidiumIodideisacell-membraneimpermeabledyewithcharacteristicexcitationmaximumat535nmandemissionmaximumat617nmwhichintercalateswithnucleicacidswithastoichiometryofonedyeper4-5basepairswithlittlesequencepreference.PropidiumIodidehasevidencedofhavingnotoxiceffectsonneurons,beingtoday’smostcommonmarkerformembraneintegrityandcellviabilitywhenappliedpriortofixation(pre-fixationPropidiumIodidestainingmethod).Thepre-fixationstaininghasbeenwidelyusedforquantitativeassessmentsofneuronalcelldeclineinmodelsofacuteneurodegeneration,visualizedasintenselylabeledPI+-pycnoticnucleiofdegeneratingneurons[1].PropidiumIodidecannotcrossthemembraneoflivecells,makingitusefultomeasurethepercentageofapoptoticcellsbyflow-cytometricanalysis.Theflowcytometricdatashowsanexcellentcorrelationwiththeresultsobtainedwithbothelectrophoreticandcolorimetricmethods.Thisnewrapid,simpleandreproduciblemethodprovesusefulforassessingapoptosisofspecificcellpopulationsinheterogeneoustissuessuchasbonemarrow,thymusandlymphnodes[2].PROTOCOLCellAssay[2]Flowcytometricanalysis:Propidiumiodideispreparedinin0.1%sodiumcitrateplus0.1%TritonX-100(50μg/mL).The200×gcentrifugedcellpelletisgentlyresuspendedin1.5mLhypotonlcfluorochromesolution(Propidiumiodide50μg/mL),in12×75polypropylenetubes.Thetubesareplacedat4°Cinthedarkovernightbeforetheflowcytometricanalysis.ThepropidiumIodidefluorescenceofindividualnucleiismeasuredusingaFACScanflowcytometer.Thenucleitraversesthelightbeamofa488nmArgonlaser.A560nmdichrolcnurror(DM570)anda600nmbandpassfilter(bandwidth35nm)areusedforcollectingtheredfluorescenceduetopropidiumIodidestainingofDNAandthedataareregisteredonalogarithmicscale[2].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•BioactMater.2022Aug11;21:20-31.•Small.2022Jul;18(30):e2202002.•JAdvRes.22December2021.•RedoxBiol.2022Feb;49:102207.•CellRep.2022Aug23;40(8):111240.Seemorecustomervalidationsonwww.MedChemE2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEREFERENCES[1].HezelM,etal.Propidiumiodidestaining:anewapplicationinfluorescencemicroscopyforanalysisofcytoarchitectureinadultanddevelopingrodentbrain.Micron.2012Oct;43(10):1031-8.[2].Arapidandsimplemethodformeasuringthymocyteapoptosisbypropidiumiodidestainingandflowcytometry.JImmunolMethods.1991Jun3;139(2):271-9.McePdfHeigh