Sulforhodamine-B-sodium-salt-Acid-Red-52-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemESulforhodamineBsodiumsaltCat.No.:HY-D0974CASNo.:3520-42-1Synonyms:AcidRed52;KitonRed620分⼦式:C₂₇H₂₉N₂NaO₇S₂分⼦量:580.65作⽤靶点:Others作⽤通路:Others储存⽅式:4°C,sealedstorage,awayfrommoistureandlight*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoistureandlight)溶解性数据体外实验DMSO:50mg/mL(86.11mM;Needultrasonic)H2O:20mg/mL(34.44mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.7222mL8.6110mL17.2221mL5mM0.3444mL1.7222mL3.4444mL10mM0.1722mL0.8611mL1.7222mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoistureandlight)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。BIOLOGICALACTIVITY⽣物活性SulforhodamineBsodiumsalt⼀种荧光染料，可⽤于定量测定培养细胞的细胞蛋⽩量。体外研究SulforhodamineB(SRB)isoftenusedasamembrane-impermeablepolartracerorusedforcelldensitydeterminationviadeterminationofcellularproteins(cytotoxicityassay).TheSRBassayhasbeenusedto1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEinexpensivelyconductvariousscreeningassaystoinvestigatecytotoxicityincellbasedstudies.ThismethodreliesonthepropertyofSRB,whichbindsstoichiometricallytoproteinsundermildacidicconditionsandthencanbeextractedusingbasicconditions;thus,theamountofbounddyecanbeusedasaproxyforcellmass,whichcanthenbeextrapolatedtomeasurecellproliferation.Theprotocolcanbedividedintofourmainsteps:preparationoftreatment,incubationofcellswithtreatmentofchoice,cellfixationandSRBstaining,andabsorbancemeasurement.Thisassayislimitedtomanualorsemiautomaticscreening,andcanbeusedinanefficientandsensitivemannertotestchemotherapeuticdrugsorsmallmoleculesinadherentcells.Italsohasapplicationsinevaluatingtheeffectsofgeneexpressionmodulation(knockdown,geneexpressionupregulation),aswellastostudytheeffectsofmiRNAreplacementoncellproliferation[1].PROTOCOLCellAssay[1]Gentlyadd25μL(96-wellformat)or5μL(384-wellformat)cold50%(wt/vol)TCAtoeachwelldirectlytomediumsupernatant,andincubatetheplatesat4°Cfor1h.Mixingisnotrequired,asthiscouldleadtosomecellsdetachingfromthebottomofthewell.Washtheplatesfourtimesbysubmergingtheplateinatubwithslow-runningtapwaterandremoveexcesswaterbygentlytappingtheplateintoapapertowel.Afterthelastwashallowtheplatetoair-dryatroomtemperature.Add50μL(96-wellformat)or20μL(384-wellformat)of0.04%(wt/vol)SRBsolutiontoeachwell.Leaveatroomtemperaturefor1handthenquicklyrinsetheplatesfourtimeswith1%(vol/vol)aceticacid(200μLfor96-wellformator30μLfor384-wellformat)toremoveunbounddye.Allowtheplatetoair-dryatroomtemperature[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•AdvMater.2022May;34(21):e2200517.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].OrellanaEA,etal.SulforhodamineB(SRB)AssayinCellCulturetoInvestigateCellProliferation.BioProtoc.2016Nov5;6(21).pii:e1984.McePdfHeightCaution:Producthasnotbeenful