L-Buthionine-S-R-sulfoximine-L-Buthionine-sulfoximine-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEL-Buthionine-(S,R)-sulfoximineCat.No.:HY-106376ACASNo.:83730-53-4Synonyms:L-Buthioninesulfoximine;L-BSO分⼦式:C₈H₁₈N₂O₃S分⼦量:222.31作⽤靶点:Ferroptosis作⽤通路:Apoptosis储存⽅式:-20°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性数据体外实验H2O:18.33mg/mL(82.45mM;Needultrasonic)DMSO:<1mg/mL(ultrasonic)(insolubleorslightlysoluble)MassSolvent1mg5mg10mgConcentration制备储备液1mM4.4982mL22.4911mL44.9822mL5mM0.8996mL4.4982mL8.9964mL10mM0.4498mL2.2491mL4.4982mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：PBS1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:100mg/mL(449.82mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY⽣物活性L-Buthionine-(S,R)-sulfoximine⼀种具有细胞渗透性的、有效的、快速起效的、不可逆的G-⾕氨酸半胱氨酸合成酶(γ-glutamylcysteinesynthetase)抑制剂，可降低细胞内⾕胱⽢肽的⽔平，其对⿊⾊素瘤、乳腺卵巢癌标本的IC50值分别为1.9μM、8.6μM和29μM。IC50&Targetγ-glutamylcysteinesynthetase[1].体外研究L-Buthionine-(S,R)-sulfoximine(BSO:50μM)treatmentfor48hrresultsina95%decreaseinZAZandM14melanomacelllineGSHlevels,anda60%decreaseinGSTenzymeactivity.GST-πproteinandmRNAlevelsaresignificantlyreducedinbothcelllines[1].L-Buthionine-(S,R)-sulfoximine(BSO)inducesoxidativestressinacellbyirreversiblyinhibitingg-glutamylcysteinesynthetase,anessentialenzymeforthesynthesisofglutathione(GSH)[2].L-Buthionine-(S,R)-sulfoximine(BSO)inducesferroptosisincancercells[3].体内研究BSOcausesanelevatedfrequencyofDNAdeletionsduringmousedevelopment.BSOtreatmentreducedGSHconcentrationinmousefetusesby55%and70%at2mMand20mMBSOdoses,respectively,comparedtountreatedmice.Co-treatmentwith2mMBSOand20mMNACdepletedGSHtoasimilarextentas2mMBSO,consistentwiththefunctionofBSOtoinhibittheg-GCSenzymeindispensableforGSHsynthesis.LikeGSH,cysteinelevelsdroppedfollowingBSOtreatment[2].户使⽤本产品发表的科研⽂献•NatCommun.2022Jul11;13(1):4007.•ActaPharmSinB.21October2021.•JColloidInterfaceSci.11August2022.•OxidMedCellLongev.2022Jan17;2022:8038857.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].FruehaufJP,etal.SelectiveandsynergisticactivityofL-S,R-buthioninesulfoximineonmalignantmelanomaisaccompaniedbydecreasedexpressionofglutathione-S-transferase.PigmentCellRes.1997Aug;10(4):236-49.[2].RelieneR,etal.GlutathionedepletionbybuthioninesulfoximineinducesDNAdeletionsinmice.Carcinogenesis.2006Feb;27(2):240-4.[3].SatoruNishizawa,etal.Lowtumorglutathionelevelasasensitivitymarkerforglutamate-cysteineligaseinhibitors.OncolLett.2018Jun;15(6):8735-8743.McePdfHeightCaution:Producthasnotbeenfullyvalid