AkaLumine-hydrochloride-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEAkaLuminehydrochlorideCat.No.:HY-112641ACASNo.:2558205-28-8分⼦式:C₁₆H₁₉ClN₂O₂S分⼦量:338.85作⽤靶点:Others作⽤通路:Others储存⽅式:-20°C,storedundernitrogen*Insolvent:-80°C,6months;-20°C,1month(storedunder

nitrogen)溶解性数据体外实验H2O:50mg/mL(147.56mM;Needultrasonic)DMSO:26.67mg/mL(78.71mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.9512mL14.7558mL29.5116mL5mM0.5902mL2.9512mL5.9023mL10mM0.2951mL1.4756mL2.9512mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(storedundernitrogen)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥4mg/mL(11.80mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥4mg/mL(11.80mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性AkaLuminehydrochloride荧光素的⼀个类似物，其对重组荧光蛋⽩的Km值为2.06μM。IC50&TargetKm:2.06μM(recombinantFlucprotein)[1].体外研究InbothLLC/lucandMDA-MB-231/luccellstreatedwithAkaLuminehydrochloride,thesignalismaximalatlowerconcentrations(by2.5μM),whereasthebioluminescencegeneratedbyD-luciferinandCycLuc1increaseasconcentrationsincreasefurther,notappearingtoreachamaximalsignalevenat250μM[1].体内研究AkaLuminehydrochloridedisplaysgreatlyenhancedsignalsemanatingfrommetastasisinthelung,asdemonstratedby8.1-foldhigherwithAkaLuminehydrochlorideascomparedwithD-luciferinadministration.ToevaluatetheadvantageofAkaLuminehydrochlorideoverCycLuc1indetectingtargetsindeeptissue,bioluminescenceimagesarecomparedbetweenAkaLuminehydrochloride-andCycLuc1-treatedmice15minafterintravenousinjectionofLLC/luccellsatthedoseof5mM,whichisthemaximumconcentrationofCycLuc1duetoitslowwatersolubility.AkaLuminehydrochlorideexhibits3.3-foldincreaseindetectionsensitivityofdisseminatedcancercellsinthelungcomparedwithCycLuc1.TheadvantageofAkaLuminehydrochlorideisfurtherconfirmedbyimagingthesamemicebearinglungmetastasisafterintraperitonealinjectionof5mMsubstratesinorderofCycLuc1andAkaLuminehydrochloride,andintheinverseorderatan8-hinterval.AkaLuminehydrochloridedisplaysaboutfourfoldincreaseinsignalsfromlungmetastasiscomparedwithCycLuc1[1].户使⽤本产品发表的科研⽂献•StemCellRes.October2021,102532.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].KuchimaruT,etal.Aluciferinanaloguegeneratingnear-infraredbioluminescenceachieveshighlysensitivedeep-tissueimaging.NatCommun.2016Jun14;7:11856.McePdfHeightCaution:Producthasnotbeenfullyvalidatedfor