DC-LC3in-D5-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEDC-LC3in-D5Cat.No.:HY-141882分⼦式:C₁₉H₂₂Cl₂N₂O₃分⼦量:397.3作⽤靶点:Autophagy作⽤通路:Autophagy储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:35.71mg/mL(89.88mM;ultrasonicandwarmingandheatto60°C)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.5170mL12.5849mL25.1699mL5mM0.5034mL2.5170mL5.0340mL10mM0.2517mL1.2585mL2.5170mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。BIOLOGICALACTIVITY⽣物活性DC-LC3in-D5通过减弱LC3B脂化作⽤⽽作为⾃噬(autophagy)抑制剂。DC-LC3in-D5与LC3B结合。DC-LC3in-D5破坏LC3B-LBP2相互作⽤，IC50为200nM。DC-LC3in-D5可能通过抑制⾃噬有助于抗HCV或肿瘤的联合治疗[1]。体外研究DC-LC3in-D5demonstrateshighselectivitytoLC3A/Bintheproteome.DC-LC3in-D5exhibitsapotentcovalentreactivityandselectivitytoLC3A/BinHeLacells[1].1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemETreatmentofHeLacellswithDC-LC3in-D5(3-30μM)resultsindisruptionofLC3Blipidation,inhibitionofautophagicvesicleformation,andaccumulationofp62[1].WesternBlotAnalysis[1]CellLine:HeLacellsConcentration:3,10,30μMIncubationTime:16hoursResult:Pre-treatedaccumulatedsignificantmorep62thanDMSO-treatedcontrolsamples.AttenuatedLC3-I/IIlipidationincellsexposedtoautophagyinducingconditions.REFERENCES[1].ShijieFan,etal.InhibitionofAutophagybyaSmallMoleculethroughCovalentModificationoftheLC3Protein.AngewChemIntEdEngl.2021Dec6;60(50):26105-26114.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792;021-5