Amygdalin-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEAmygdalinCat.No.:HY-N0190CASNo.:29883-15-6分⼦式:C₂₀H₂₇NO₁₁分⼦量:457.43作⽤靶点:Others作⽤通路:Others储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:≥75mg/mL(163.96mM)H2O:50mg/mL(109.31mM;Needultrasonic)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM2.1861mL10.9306mL21.8613mL5mM0.4372mL2.1861mL4.3723mL10mM0.2186mL1.0931mL2.1861mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(5.47mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性扁桃素从蔷薇果实的果核中分离的植物葡萄糖苷，例如杏，桃，杏仁，樱桃和李⼦。体外研究Amygdalinhasantitumoractivity.Someadvanceshadbeenmadeontheantitumormechanismofamygdalin[1].Amygdalindownregulatesespeciallygenesbelongingtocellcyclecategory:exonuclease1,ATP-bindingcassette,sub-familyF,member2,MRE11meioticrecombination11homologA,topoisomerase(DNA)I,andFK506bindingprotein12-rapamycin-associatedprotein1.RT-PCRanalysisrevealsthatmRNAlevelsofthesegenesarealsodecreasedbyamygdalintreatmentinSNU-C4humancoloncancercells[2].体内研究Amygdaliniseffectiveatalleviatinginflammatorypainandthatitcanbeusedasananalgesicwithanti-nociceptiveandanti-inflammatoryactivities.Theintramuscularinjectionofamygdalinsignificantlyreducedtheformalin-inducedtonicpaininbothearly(theinitial10minafterformalininjection)andlatephases(10-30minfollowingtheinitialformalininjection).Duringthelatephase,amygdalinreducestheformalin-inducedpaininadose-dependentmannerinadoserangelessthan1mg/kg[3].PROTOCOLCellAssay[2]CellviabilityisdeterminedbyMTTassay.Cellsareseededintriplicateataconcentrationof1×105cells/wellona96-wellplate.SNU-C4cellsaretreatedwithamygdalinatconcentrationsof0.25,0.5,2.5,and5mg/mLfor24h.AfterMTTisaddedtoeachgroup,thecellsareincubatedfor4h.Then,theyarefurtherincubatedfor1h,includingthesolutioninwhichMTTisdissolved[2].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.AnimalRats:Theamygdalinpowderisdissolvedinsalineanddilutedwithappropriatemedium.MaleAdministration[3]Sprague–Dawleyratsweighing230-250gareusedforthisexperiment.50mLof5%formalinareinjectedtoproducefomalin-inducedpainintherats.Thirtyminutesbeforetheformalininjectiontoinducepain,theratsaregivenanintramuscularinjectionofamygdalinsolution(0.1,0.5,1.0,10mg/kg),orsalineasavehiclecontrol[3].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•BiochemBiophysResCommun.5March2022.•EvidBasedComplementAlternatMed.2020May23;2020:8607931.Seemorecustomervalidationsonwww.MedChemEREFERENCES2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[1].SongZ,etal.Advancedresearchonanti-tumoreffectsofamygdalin.JCancerResTher.2014Aug;10Suppl1:3-7.[2].ParkHJ,etal.AmygdalininhibitsgenesrelatedtocellcycleinSNU-C4humancoloncancercells.WorldJGastroenterol.2005Sep7;11(33):5156-61.[3].HwangHJ,etal.AntinociceptiveeffectofamygdalinisolatedfromPrunusarmeniacaonformalin-inducedpaininrats.BiolPharmBull.2008Aug;31(8):1559-64.McePdfHeightCaution