D-Galactose-D-plus-Galactose-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemED-GalactoseCat.No.:HY-N0210CASNo.:59-23-4Synonyms:D-(+)-Galactose分⼦式:C₆H₁₂O₆分⼦量:180.16作⽤靶点:EndogenousMetabolite作⽤通路:MetabolicEnzyme/Protease储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验H2O:62.5mg/mL(346.91mM;ultrasonicandwarmingandheatto60°C)DMSO:50mg/mL(277.53mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM5.5506mL27.7531mL55.5062mL5mM1.1101mL5.5506mL11.1012mL10mM0.5551mL2.7753mL5.5506mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(13.88mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(13.88mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(13.88mM);Clearsolution4.请依序添加每种溶剂：PBSSolubility:100mg/mL(555.06mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY⽣物活性D-Galactose天然的⼰糖，葡萄糖的C-4差向异构体。IC50&TargetHumanEndogenousMetabolite体外研究Galactoseisimportantforthesurvivalandvirulenceofbacteria.InEscherichiacoligalactoseisutilizedbytheLeloirpathway.Twoanomersofd-galactoseareusedfordifferentpurposes,α-d-galactoseasacarbonsourceandβ-d-galactoseforinductionofUDP-galactosesynthesisforbiosyntheticglycosylation[1].体内研究ChronicD-galactoseexposureinducesneurodegenerationbyenhancingcaspase-mediatedapoptosisandinhibitingneurogenesisandneuronmigrationinmice,aswellasincreasingoxidativedamage.Inaddition,D-galactose-inducedtoxicityinmiceisausefulmodelforstudyingthemechanismsofneurodegenerationandneuroprotectivedrugsandagents[2].D-galactosegivenbyoralroutecausescognitiveimpairmentsinratswhichareaccompaniedbyoxidativedamage.Cognitiveimpairmentsisobservedintheopen-fieldtestinthe4thand6thweeksafterd-galadministration,aswellasanimpairmentinspatialmemoryintheradialmazetestafterthe6thweekofd-galadministration[3].PROTOCOLAnimalRats:D-galactoseisdissolvedinwaterforadministrationatthedoseof100mg/kgofbodyweight,andgivenAdministration[2][3]byoralgavage,onceaday,overaperiodof1,2,4,6or8weeks.Animalsarerandomizedintotwogroups:controlanimals(receivingwaterbyoralgavage)ord-galanimals(receivingD-galactosebyoralgavage).Thebehavioraltestsandbiochemicalanalysisareundertakenonthe1st,2nd,4th,6thand8thweeksafterthelastadministrationofd-gal[3].Mice:MaleadultC57BL/6micearerandomlydividedintothreegroups(control,D-galactose,andD-galactoseplusα-LA).D-galactose(100mg/kg)isinjectedsubcutaneously(s.c.)dailyintomicefor7weeks.α-LA(100mg/kgbodyweight)isinjectedperitoneally(i.p.)dailyconcomitantlyfor7weeks.Allcontrolanimalsaregivensaline[2].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE•SignalTransductTargetTher.2022Sep1;7(1):303.•PLoSBiol.2018Oct18;16(10):e2006483.•Cells.2022,11(20),3270.•IntJMolSci.2022Sep2;23(17):10045.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].CsiszovszkiZ,etal.StructureandfunctionoftheD-galactosenetworkinenterobacteria.MBio.2011Jun28;2(4):e00053-11.[2].CuiX,etal.ChronicsystemicD-galactoseexposureinducesmemoryloss,neurodegeneration,andoxidativedamageinmice:protectiveeffectsofR-alpha-lipoicacid.JNeurosciRes.2006Aug15;84(3):647-54.[3].BudniJ,etal.Oraladministrationofd-galactoseinducescognitiveimpairmentsandoxidativedamageinrats.BehavBrainRes.2016Apr1;302:35-43.M