捏脊疗法对免疫抑制模型大鼠T淋巴细胞亚群及T-bet-GATA-3、IFN-γ-IL-4 mRNA的影响

捏脊疗法对免疫抑制模型大鼠T淋巴细胞亚群及T-bet-GATA-3、IFN-γ-IL-4mRNA的影响摘要：本研究旨在探讨捏脊疗法对免疫抑制模型大鼠T淋巴细胞亚群及T-bet/GATA-3、IFN-γ/IL-4mRNA的影响。将45只大鼠随机分为正常组、模型组和捏脊疗法组，模型组和捏脊疗法组先注射环磷酰胺制备免疫抑制模型。对捏脊疗法组进行22天应用捏脊疗法治疗，通过流式细胞术、实时荧光定量PCR技术检测T淋巴细胞亚群及T-bet/GATA-3、IFN-γ/IL-4mRNA的水平。结果显示：与正常组相比，模型组T淋巴细胞比例下降，CD4+细胞、Th1和Th2细胞比例减少，T-bet和IFN-γmRNA表达下降，GATA-3和IL-4mRNA表达升高；治疗后，捏脊疗法组T淋巴细胞比例、CD4+细胞比例、Th1和Th2细胞比例均高于模型组，T-bet和IFN-γmRNA表达升高，GATA-3和IL-4mRNA表达下降。说明捏脊疗法可以增强免疫功能，促进Th1/Th2细胞平衡，从而对免疫抑制模型大鼠的治疗产生积极作用。

关键词：捏脊疗法；免疫抑制模型；T淋巴细胞亚群；T-bet/GATA-3；IFN-γ/IL-4mRNA

Introduction

捏脊疗法是传统中医经过长期实践发展而成的一项治疗技术，通过按摩、推拿等手法调节身体疏松、缓解疼痛的方法。近年来，随着人们对健康的关注不断提升，捏脊疗法作为一种安全、经济、易行的治疗方式，在临床上得到广泛应用。同时，由于现代社会中环境污染等因素的影响，人们普遍存在着免疫力下降的问题，捏脊疗法作为一种潜在的免疫调节手段备受研究者关注。本研究旨在探究捏脊疗法对免疫抑制模型大鼠T淋巴细胞亚群及T-bet/GATA-3、IFN-γ/IL-4mRNA的影响，为其临床应用提供一定的实验依据。

Materialsandmethods

动物模型：将45只雄性SpragueDawley大鼠随机分为正常组、模型组和捏脊疗法组，模型组和捏脊疗法组先注射环磷酰胺制备免疫抑制模型。

捏脊疗法：治疗期为22天，每天进行1次捏脊疗法操作，每次20分钟。

流式细胞术：采用流式细胞术检测T淋巴细胞亚群比例。

实时荧光定量PCR技术：采用实时荧光定量PCR技术检测T-bet、GATA-3、IFN-γ、IL-4mRNA表达水平。

Results

与正常组相比，模型组T淋巴细胞比例下降，CD4+细胞、Th1和Th2细胞比例减少，T-bet和IFN-γmRNA表达下降，GATA-3和IL-4mRNA表达升高。治疗后，捏脊疗法组T淋巴细胞比例、CD4+细胞比例、Th1和Th2细胞比例均高于模型组，T-bet和IFN-γmRNA表达升高，GATA-3和IL-4mRNA表达下降。

Conclusion

捏脊疗法可以增强免疫功能，促进Th1/Th2细胞平衡，从而对免疫抑制模型大鼠的治疗产生积极作用Discussion

Thepresentstudyinvestigatedtheeffectsofspinalmanipulationtherapyontheimmunefunctionofratswithanimmunesuppressionmodel.TheresultsdemonstratedthatspinalmanipulationtherapycouldenhancetheimmunefunctionofratsandimprovethebalancebetweenTh1andTh2cells.

Immunesuppression,definedasadecreasedimmuneresponsetoantigens,canincreasesusceptibilitytoinfectionsandtumors.Inthepresentstudy,immunesuppressionwasinducedinratsbytheadministrationofcyclophosphamide.ThereducedTlymphocyteproportionandTh1/Th2balancechangesobservedintheimmunesuppressionmodelgroupwereindicativeofimmunesuppression.However,thespinalmanipulationtherapygroupshowedanincreaseinTlymphocyteproportion,CD4+cellproportion,andTh1/Th2balance,leadingtoanenhancedimmuneresponse.

T-betandGATA-3aretranscriptionfactorsthatplayacriticalroleinthedifferentiationofTh1andTh2cells,respectively.T-betpromotesthedifferentiationofTh1cellsandtheproductionofIFN-γ,whileGATA-3promotesthedifferentiationofTh2cellsandtheproductionofIL-4.ThepresentstudyshowedthattheexpressionofT-betandIFN-γmRNAintheimmunesuppressionmodelgroupwassignificantlylowerthanthatinthenormalgroup,whiletheexpressionofGATA-3andIL-4mRNAwassignificantlyhigher.ThissuggeststhatimmunesuppressionleadstoashifttowardaTh2phenotype.However,theexpressionofT-betandIFN-γmRNAinthespinalmanipulationtherapygroupwassignificantlyhigherthanthatintheimmunesuppressionmodelgroup,whiletheexpressionofGATA-3andIL-4mRNAwassignificantlylower.ThissuggeststhatspinalmanipulationtherapycanpromotethedifferentiationofTh1cellsandtheproductionofTh1cytokineswhilesuppressingTh2responses.

IL-4andIFN-γareimportantcytokinesproducedbyTh2andTh1cells,respectively.ThepresentstudyshowedthattheexpressionofIL-4mRNAwassignificantlyincreasedintheimmunesuppressionmodelgroup,whiletheexpressionofIFN-γmRNAwassignificantlydecreased.However,theexpressionofIFN-γmRNAwassignificantlyincreasedinthespinalmanipulationtherapygroup,whiletheexpressionofIL-4mRNAwassignificantlydecreased.TheseresultssuggestthatspinalmanipulationtherapycanpromotetheproductionofIFN-γandsuppresstheproductionofIL-4,leadingtoashifttowardaTh1phenotype.

Insummary,thepresentstudydemonstratedthatspinalmanipulationtherapycanenhancetheimmunefunctionofratsbypromotingthedifferentiationofTh1cellsandsuppressingthedifferentiationofTh2cells.Thesefindingsprovideexperimentalevidencefortheclinicalapplicationofspinalmanipulationtherapyinthetreatmentofimmune-relateddiseases.However,furtherstudiesarerequiredtoinvestigatetheunderlyingmechanismsofspinalmanipulationtherapyontheimmunesystemInadditiontotheimmunesystem,spinalmanipulationtherapyhasbeenreportedtohavebeneficialeffectsonvariousothersystemsandconditions.Forexample,ithasbeenshowntobeeffectiveinthemanagementoflowbackpain,neckpain,headaches,andothermusculoskeletaldisorders.Moreover,spinalmanipulationtherapyhasbeensuggestedtohaveapositiveimpactonpsychologicalhealth,suchasreducingstressandanxietylevels.

Severalstudieshaveinvestigatedtheunderlyingmechanismsofspinalmanipulationtherapy,includingitseffectsonpainprocessing,sensoryprocessing,andneuromuscularfunction.However,moreresearchisstillneededtoelucidatethemechanismsbywhichspinalmanipulationtherapyexertsitseffectsondifferentphysiologicalsystems.

Onepotentialmechanismisthroughthemodulationoftheautonomicnervoussystem,whichisresponsibleforregulatingmanybodilyfunctions,includingheartrate,bloodpressure,digestion,andstressresponse.Spinalmanipulationtherapyhasbeenshowntohaveacalmingeffectonthesympatheticnervoussystem,whichisresponsibleforthefight-or-flightresponse,andtoenhancetheactivityoftheparasympatheticnervoussystem,whichisresponsiblefortherest-and-digestresponse.Thiscouldexplainwhyspinalmanipulationtherapyhasbeenshowntoreducestressandanxietylevels,enhancerelaxation,andimprovesleepquality.

Inaddition,spinalmanipulationtherapyhasbeensuggestedtomodulatethemovementpatternsandmotorcontrolofthemusculoskeletalsystem,leadingtoimprovedfunctionandreducedpain.Thisisthoughttooccurthroughtheenhancementofproprioception,whichistheabilitytosensetheposition,movement,andforceofthebodypartswithoutrelyingonvisualcues.Proprioceptionisessentialforcoordinatedmovement,balance,andposture,andisoftenimpairedinindividualswithmusculoskeletalpainorinjury.Spinalmanipulationtherapyhasbeenshowntoenhanceproprioceptionbystimulatingthesensoryreceptorsinthejoints,muscles,andligamentsofthespine,leadingtoimprovedmotorcontrolandfunctionalperformance.

Inconclusion,spinalmanipulationtherapyhasbeenshowntohavebeneficialeffectsonvariousphysiologicalsystems,includingtheimmunesystem,musculoskeletalsystem,andautonomicnervoussystem.Althoughthemechanismsofactionarenotfullyunderstood,evidencesuggeststhatspinalmanipulationtherapymaymodulatethesensoryandmotorsystemsofthebody,leadingtoimprovedfunctionandhealthoutcomes.FurtherresearchisneededtobetterunderstandtheunderlyingmechanismsandtooptimizetheclinicalapplicationofspinalmanipulationtherapyfordifferentconditionsSpinalmanipulationtherapy(SMT)isawidelyacceptedtreatmentforspinaldisorders,includinglowbackpain,neckpain,andheadaches.TheefficacyofSMThasbeendemonstratedthroughalargenumberofclinicaltrials,systematicreviews,andmeta-analyses.However,theunderlyingmechanismsbywhichSMTproducesitstherapeuticeffectsarenotfullyunderstood.

OnepossiblemechanismbywhichSMTworksisthroughtheactivationoftheimmunesystem.Theimmunesystemplaysacriticalroleinthepathophysiologyofmanyspinaldisorders,includingdiscdegeneration,discherniation,andvertebralfracture.AgrowingbodyofevidencesuggeststhatSMTcanstimulatetheimmunesystem,leadingtoanincreaseintheproductionofcytokinesandchemokinesthatpromotetissuerepairandregeneration.

AnothermechanismbywhichSMTmayworkisthroughitseffectsonthemusculoskeletalsystem.SMThasbeenshowntoimprovejointmobility,reducemuscletension,andincreasemusclestrength.Theseeffectsmaybemediatedbytheactivationofmechanoreceptorsinthemusculoskeletalsystem,leadingtoanimprovementinproprioceptionandmotorcontrol.

Inaddition,SMTmaymodulatetheautonomicnervoussystem,leadingtoareductioninsympatheticactivityandanincreaseinparasympatheticactivity.ThismayexplaintheobservedreductioninpainandstresslevelsfollowingSMT.

AlthoughthemechanismsofactionofSMTarenotfullyunderstood,thereisgrowingevidencethatSMTcanproducesignificanttherapeuticbenefitsforawiderangeofspinaldisorders.However,furtherresearchisneededtooptimizetheclinicalapplicationofSMT,includingtheidentificationofpatientsubpopulationsthataremostlik