医学微生物学 细菌培养(英文)

BacterialCultivationprincipalToobtaininformationofbacterialbiologiccharacteristics,itisnecessarytoobservebacteriainculture.Inordertocultivatethemsuccessfully,wemustprovidethemwithsuitablenutrients:protein,carbohydrates,vitamins,andsoon.Culturemedium:Isthemixtureofvariousnutrientsthatissuitableforthegrowthofmicroorganisms.2TypesofculturemediaBasedonthe

function

andchemicalcomponentsBasedonthephysicalstate3BasedonthefunctionandchemicalcomponentsBasicmediumEnrichedmediumSelectivemediumDifferentialmedium4BasedonthephysicalstateLiquidmediumWithoutagarFortheproliferationofbacteriaSolidmedium1.5-2.5%agarFortheisolationandidentificationofbacteria,preservationofbacteriaE.g..SlantandplatesSemisolidmedium0.3-0.5%agarFortheobservationofbacterialmotility5Inoculationandtransfertechniques

methods:Streak-platetechniqueSlantinoculationLiquidmediuminoculationSemisolidmediuminoculation61.streak-platetechnique7Materials(streakplate)

CulturemediumAgarplateBacterialstrains

AmixtureofS.aureus&E.coli(金大混合菌)Inoculateloop,alcohollamp,markingpencil,andsoon8Inoculationloopisheateduntilitisred-hotTubeenclosureisremovedmouthoftubeisflamedAloopfuloforganismisremovedfromtubeLoopisremovedfromthecultureandtubeisflamedTubeenclosureisreturnedtube9Four-areastreakplatetechniquewithgentlypressure,don’tscratchthesurfaceoftheagar10BacterialgrowthpatternsInagarplatemediumConfluentgrowth/smear;Colony:Aclusterofmicroorganismsgrowingonasolidmedium.Itisdirectlyvisibleandarisesfromasinglecell.Fortheisolationofbacteria

11ConfluentgrowthatbeginningofstreakIsolatedofcoloniesattheendofstreakAtleasttwotypesofcoloniesDescribethesize,shape,pigment,margin,andsoon122.Slantinoculation13MaterialsCulturemediumslantmediumBacterialstrains

E.coli(大),Shigella(痢),

S.aureus(金),

anyoneInoculateloop,alcohollamp,markingpencil,andsoon14SlantinoculationStreakastraightlineonthesurfaceoftheslant,thenstreakSlineonthesurfaceofslantwithgentlypressure15BacterialgrowthpatternsInslantmdiumUniformlawnForpreservationofbacteria163.Liquidmediuminoculation17MaterialsCulturemediumliquidmediumBacterialstrains

E.coli(大),Shigella(痢),

S.aureus(金),

anyone

Inoculateloop,alcohollamp,markingpencil,andsoon18LiquidmediuminoculationGentlyrubtheloopagainstthewallofthetube19BacterialgrowthpatternsInliquidmedium:SuperficialgrowthTurbidity/diffusePrecipitategrowingFortheproliferationofbacteria204.Semisolidmediuminoculation21MaterialsCulturemediumsemisolidmediumBacterialstrains

E.coli(大),Shigella(痢),anyoneinoculateneedle,alcohollamp,markingpencil,andsoon22SemisolidmediuminoculationStabtheneedleintothesemisolidmedium,Thenretracethewayback23Bacterialgrowthpatterns•Insemisolidmedium:–Onlygrowalongthelineofinoculation–GrowdiffuselyForidentificationofthemotility241.Heatloop(needle)for5seconds2.Collectaloopfuloforganisms&removeloop.3.Replacecapandplacetubeinrack.4.Transfertobrothtube,agarslant,agarplate.5.Heatloop252students/groupInoculationmethodCulturemediaBacterialstrainsStreakplate2plates(1plate/student)Amixedliquidof

S.aureus&E.coliSlantinoculation2slants(1slant/student)

E.coli

Liquidmedium1liquidmediumE.coli

Inoculation1liquidmediumS.aureus

Semisolidmedium1semisolidmediumE.coli

Inoculation1semisolidmediumshigella

NOTEMark

yourfreshlyinoculatedplate/tubesShakeupthemixedliquidofbacterialstrainsbeforeusingit

2627Atthetimeof