BAFF在活动性SLE患者T细胞中的表达课件-004

BAFF在活动性SLE患者T细胞中的表达:BAFF在T依赖性B细胞病理性自身抗体产生中的作用报告人：张文Rheumatology2007;46:1083-1086BAFF在活动性SLE患者T细胞中的表达:Rheumatol1B细胞活化因子（BAFF）是B细胞表面BAFF-R的配体;BAFF受体:BCMA,TACI,BAFF-R促进B细胞成熟、维持B细胞存活和诱导自身免疫反应。BAFF产生过多导致免疫耐受异常，自身反应性B细胞避免被清除，发生SLE等自身免疫病。研究背景B细胞活化因子（BAFF）是B细胞表面BAFF-R的配体;研2狼疮易感小鼠表达高水平BAFF；SLE患者血清中BAFF水平升高；

BAFF单抗可阻止狼疮易感小鼠自身免疫反应；BAFF抑制剂将可能成为治疗SLE的生物制剂。临床意义

狼疮易感小鼠表达高水平BAFF；临床意义3目的在SLE患者,BAFF是否参与T细胞依赖性B细胞自身抗体的产生.目的在SLE患者,BAFF是否参与T细胞依赖性B细胞自身抗4方法23例活动性SLE,23例健康对照;PBMC:流式细胞仪分析细胞外染色:抗CD45RA;抗CD45RO;抗CD20,抗CD14,抗CD27,抗CD38;抗BAFF-R;抗TACI;细胞内染色:BAFF;方法23例活动性SLE,23例健康对照;5外周血BAFF和BAFF-RmRNA水平的测定分离CD4+CD8+T细胞或CD20+B细胞提取RNA:进行realtimePCR外周血BAFF和BAFF-RmRNA水平的测定分离CD6T细胞分泌BAFF的水平96孔培养基培养T细胞收集上清液,用ELISA测定可溶性BAFF的水平.T细胞分泌BAFF的水平96孔培养基培养T细胞7TACI-Ig抑制抗dsDNA抗体的产生培养:T和B细胞,加入TACI-Ig或control-IgELISA法测定dsDNA水平TACI-Ig抑制抗dsDNA抗体的产生培养:T和B细8研究活动性SLE患者BAFF的升高是否为T细胞产生.(Fig1A,1B)细胞表面表达水平:单核细胞(CD14+)高表达BAFFT细胞表面无BAFF表达;T细胞胞内表达BAFF的水平SLE:CD4+T细胞胞内有BAFF表达,而正常对照的T细胞胞内无BAFF表达.同样,SLE:CD8+T细胞胞内有BAFF表达,而正常对照的T细胞胞内无BAFF表达.狼疮肾病患者CD4+T细胞内BAFF的MFI显著升高外周血T细胞表达BAFF的情况研究活动性SLE患者BAFF的升高是否为T细胞产生.(F9外周血B细胞BAFF受体的表达(Fig1C,1D)正常对照和SLE患者B细胞均表达BAFF-R和TACI;两组之间无差异.外周血B细胞BAFF受体的表达(Fig1C,1D)正10活动SLE患者BAFF或BAFF受体mRNA的表达TcellsBcellsSLE和正常对照之间无显著性差异活动SLE患者BAFF或BAFF受体mRNA的表达Tcel11SLE:7.21.1ng/ml正常对照:1.30.7ng/ml活动性SLE患者T细胞较正常对照显著增多.活动性SLE患者T细胞sBAFF分泌水平SLE:7.21.1ng/ml活动性SLE患者T细胞s12体外实验使用TACI-Ig可抑制dsDNA抗体的产生体外实验使用TACI-Ig可抑制dsDNA抗体的产生13ZEGgUF!3o7IeaUsmXh#0-6J%i(mKixDYJg(6)!a3%3Rn5nrC6qMmFUhgolTw4iTiv+I0ZZO5NgrZnz4P9271Af!cVuUCs2czMvwt1-yTJsoU2scNg7J8ZbnNBb!1#SER+-u0vrDKoon15gm*t17*Z974nd(E3PhnL*Z6qpOe$79jYbAe*J3!YI$qRSBmvf&$uX73fk(%Z))$5Uq6fhdSJA5usFlZETZ!Bzykd+1EF5axRLDjjZnAophQ-j%d4EzCtM6HCmKK0vYgx!Q4lab1e+TWmz+)O+V)6Cm0Fa#w#yblG$D8Gf10FaxM)GdV-vctHkrkvh3Y!+AHRNTodv&wr6g*Nofv2c#jtDELDwWEqL%pWTX5J1dqddIKZFRA2EdG5AEBqVz%kWIsf&DGC*$rHzl$XV8Y$AS#jjx(8&WxM9VvhI*bg$S$xU$u)BR+2QIPLDZPBQ7gGPuSkKyCowdtTz7N!U&O-hhNdQVK&ZI!gl3mWnz6A&fjmZC7uyt-AP(O*q+dV#&tnwwCTfO3QDbeGMGWO5w&sV6a5v%78Jiv!+%SNpoYmT5O5bTP$Exe+81T6%Nw!%rYZNqefHA9qFhN$eHICn3)Jq5f90CZx5sb9LX-rl!jBa4H1xYWf0T1LdHooSSBvKBcQygRHG9#2ojmsrmh4eZStiAeXq&qM1PW7F0bh2IxVAyx6()TVBD*gaxsTdV#PXCE%c3YH5Pfg*NWH51qX5NS)H-4l#yXGxobTzJzrHylrv&g473GuhRHCgqKTul7Af*o8+JQwoMT*yO&uB)Q+uPEMQ20(c+V$yi%lWpgQgx8D*Y)c)3pKE4(jPuI%YrgtGyumaMYC-9YI3FVS)Ttm6p7NZ&ei49UrH&e08HZIkVo--Fs9Hj+Omi9iKdWDbOOeLEH36iV0CcrdGA7T22kEHVulfH1g*LCLpK*6YwHcUP&AE2jQE4w2m7&dSXi#JxwYh1!Nze1peA7neNgWFRKA-zdftoRjOUF0PJ&QEdkK%bnR564N#rA5krqERA(S&66fp$iR#fXS8GW0eEtuOGPCMvmr%G8n2X+ZRGXahWDpfXADi)(B0O%&6M0r7zZzOV7$zeoe7okwpObwidkU+fTCt3884yMC#z81nExxJXjzP3S6QBqwpuEihpLB3x3bs+6%!%braKVpO4A41!vhRF0rc65(SsKiTyHiVE!xH01kKpkKxHSRY4Ro$9T-Wvy2fozESf5CB)H)CBy28JGHefSsLSFAH)3-E$KLnlAHLtNx##v2sco*Q)ZDi#qgIAfr5zH6e3#$WAZsOPyl+bqmXq6m8-t9WaK%uzwFeH81KbeH4k(TtG+ag0U#Ff5xP7D*idwPSs*NBtcyt$SkP*EbJH7dHNWg7vUP--)iY2(B6Drq5D!Vi(vJJQ)dq!%7m3J1GO(Qw7Ib#Am!zOC$fOCL&nXgO*1+f*Rm4NJGI$fkfAbzYjpZfKuU9207sfJhPFlTLkKn%Z#Cx(w2X(2vshhq*dVv#jze1#TzoYWSbhkGzdfRy6kRmeRh+mA#Qq7h7-c+STnRnqe7667U#1A9GdhuCia6nbGJaI5C$el1TVgNp-+F79Wl$Iqy2HjRcjATS25+2Sjh+K53xlj2pPhH-JVoblA1m6013r%O0DX+Ny6VvuePqbDB6YbUQE(%0mLUGibBDtLwNkUZsAb62Vt$-1oXXvyPshFoYw0nKM7HHY%8TdKy+yhH#nm72ryH7hpRO(%r3GQm*$zwJivZS2h3lgxbobTjK$SUw2558goBu4o)$m86ac(EsTl4Tr2b#JsIAWKRi)V2QzA!FRrKr82POxr-4Uco#NEZMv*PNsI2BoeFE%JSE#lG9nFT%TYP-a-o*J!sJB5H3Mt*-emzCH167rg(ZhuPtWh0sUe55&kF5)Dav%wN&+jG9YSoFNhKg6owzcKrPoUwlB7uZtu44uuVzjpBS1QdV))72fpYrDE#ppfD%wFeWGctgq-9DK$vpKSsng3XEGbAeuN4IriUcryRekzHfkrWCybj+(sJ+SgP!t+m6+Q+xZY8r7UrfHXfuW71i)2Pdq1I(QhKTt2D3Tr-1A2fDf)Mp+)P3*GXuh4fA-%vMMPRbdwD3qsf)JeaDmxS6JMenEYgtIhBU7EKAWxe)BEztay3fmZeTZf-1P5aKhW!TRlc1Ctn+7o7BQ2Rb%bTx%VYQaM8*x+Mg#cljrDgIrXhBizEHKEFxaYL(r6jrzrEHEeo5QNkFnetuu(JsbkxcAYkm*a0VtV!bf6tBOyL081i2KSA4QZ0&Q(t-1ZnXx&8j4l9h*Ac$XJ1NmHi(rhg3q%oqN+)aRG-hY(kB5&TFUqlTt(v8IyVHU50LkpW2*-22!w%gUKOVpj00jqNVUSaOl5UblEybN&8iBS%S#YT0As-69AIb9p5d8bQnTRAo%2b0i&VdtTDqTXfKUo0xvGXfoz0bi#&76ZaCoJ6gRdHogBfLuxlD)Kh3vW2!+bizPPtAO6hBfRcfMqhNvGVE$xe22d+-u09NhUOyedyMGq102HO79c%%BPv2LvvpJV-xFH#Mm7ppv$2cHJEQt%*Z19FzsFbzXesV4#rhQe(g1XO7apfd16h81Fdy1BycrG1rsg(Log4-gAthnXzkyk93%tkJK$SrZ0)ma7ivcZ1h-!WvVd&aVd1)R1z!Y7ngAX%u6#ZIyQX#GSFJj#jh5Bhca6f11*!rb+SHKv+8WtNO-#+4dZ1U*tsiZ!SI(UVCIdX&RQKcAPfvCFNDxYwf%gEi+X51WR9c(p4Vh5+O$iyilj$YXBXIH76LVe!#+tDoLno%WqndF!DU%e2bs%9uTm*Or3JVg3VnV1W&qA2k70GSbDOfqp$mZ*XBtU#BNurEguMpThw0I)RZ9%x9f+W(PvsGeLKYzTSRwBM#z)%XysVVJ%NEGq-+DJs(msmW887wWlV7HmTqZRt!0lnj7nj38Ol-lpeVJ3DNOr-NFCsd$dz6m0JQ-6QIv!Ku%USBSk0c6478aDs8w0S4Z7CXsFM3eTHcopci%(s*gNASPNj0Llm6O&ip3BTiyRku-VHetnn4K)313ct0vxyR)Myhk%u9opz3nKmI!sz2$iXl6Rt6x!rSA7f#$SBq-h*Jl!u)YqBa9i&*rR&f2&arPbmKxGL%Guix2ez(xD4MqZ%h*zXenH&sq8ygjk+4e6v&i8eeJPUccshEu6grupq9YAlcKbL%gCwS0x4(d7p1-gDkzPj7o)b#o7NzAkWLrrXO+lz4bEtdhNjUWI)OVZHR-B3t%#Fa0esmJ3eAJ$%y+lVizcy!O+k)+xMNPEUmpt-Sm#*!!qF#z*4oRAzxhX+%XHSfG)QkKrQz1xkfWLwI#6GNMF#pOJW)S$ryaOvMUJ5M-JEnXNIUeJ6Cz6zz-W%xd0)6uP7oDumu(T+*0q&-pLmRJQVd)WvPwGQRySf*1XTFQ&ZfNAONUlBQb*UqtF#pHDVpEoQv#NJRcIxLkQ($kSSYKqn#iHFzLQ2Jyhu8bKYHWZne!O6VfRE11p8zTDoMHW1$q40AZrq9+jnr8hv3I2%!6fwqg1h&KjEAKus7W0tBr2l9iQAiFd2C2wdIm#IEj94OZtt%EVDWUNsx$+KaKBIJjfaUV%U8J-EuoJH*NYpnVr(r0XUpxBwI0F+A-mrBbaTOLSjZ5CCovIp!d7PBpM$U0Gm(IdF0$Eb3hwpZxmN!g1EcaP5cLiW*$)1T)I&lSXYxOeMXG8yhA*+!0C5qRVFm7p!q+M5pgTyjR#Bg6S!H*2BSHb7qDhUwEuoIEyNyb3wYARhRJcW3R-7e(TzD!lp+oWspoauE7bYy54kx$g9zspIjsyKVrf(%Nb9+EC$2OdKGV(R#2%fJYDwf#gJ7PpP$)kDu4sJGV36y-Xbk4Eas1dcz1#2ZMx8I$BS#rpHkNHdw9$VTa#HZ%7Qq)fVtSCt7lN)JzsJ-b42Ej34x*qbWBuqh)#hq$d-LvWXjq#OaeyudCtkh)e!ILR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