r型对映体精甲霜灵的分离测定

r型对映体精甲霜灵的分离测定

1u2004范围金属传感器-会议（r）-与颗粒形成一个整体的圆形颗粒。m-向心分离特征。大多数微芯片结构（r）-22-n-（2，6-官方消息）-meth石雕鱼饵官[范]。Fortheresidueanalysisofmetalaxyl-Minplantmaterials,amixedelutingsolutionofn-hexaneanddiethyletherarealwaysusedtoelutethechemicalextractsthroughanaluminachromatographiccolumn.Butdiethyletherisapungentsolventandharmtotheworkersonoperation,soitisnecessarytofindoutasubstitutetoensuresafety.Themodificationmethodwasintroducedasfollows.2杏仁杏仁2.1iiiityvophict液Litchifleshwasusedastheplantmaterial,andothermaterialsincludeacidicaluminawithactivityV(85%activitygradeI:15%water(w/w))aschromatographiccolumn,andmetalaxyl-Masreferencesubstanceforstandardwiththeconcentrationof1.5×10-5g/mlinn-hexaneforrecoverytest.ElutingsolutionA:n-hexane:diethylether=1:1(v/v);B:n-hexane:dichloromethane=1:1(v/v).2.2我2.2.1案例5:美国as对于shpondingjar的介绍Samplesof20guncontaminatedlitchifleshwerehomogenizedandtransferredintoa500mlwidemouthjar,and80mlmethanolwasaddedandthenshakenthejarfortwohours.ThesuspensionwasfilteredinsuctionthroughBuchnerfunnel,thejarwasrinsedwith50mlmethanolandthefiltrateswerecombined.2.2.2阶段roxy风险最适生长细胞最适比例的确定A1000mlseparatoryfunnelwaspreparedwith25mlsaturatedaqueousNaClsolution.Thefiltratewasaddedandshakenwith50mldichloromethanefor2mineachtimeandwaitedforphasestoseparate.Thelowerorganicphasewasputintoroundflask,andthesupernatantwasseparatedwith40mldichloromethanefortwotimesandalltheorganicextractswerecombined.Themixedextractwasthenevaporatedwitharotatingevaporatortoleaveabout1mlatwaterbathat45℃,andrinsedwithdichloromethane,andadjustedto5ml.Onemlofmetalaxyl-Mstandardsolution(1.5×10-5g/ml)wasaddedintothelastadjustedsolution(fortificationlevel0.75mg/kg),andunfortifiedmetalaxyl-Mlitchifleshwasusedascontrol.2.2.3so阶段so实施so阶段Na2SO43cm,acidicalumina(gradeV)5gandNa2SO43cmwerefilledintochromatographicglasscolumnwithlength21cmandinnerdiameter8mminturn.Thecolumnwasprewashedby20mlsolutionAandsolutionB,respectively.Thentheextractsolutionfortifiedwithmetalaxyl-MwastransferredtothecolumnwhenliquidsurfacewenttothetopofNa2SO4.ThecolumnwaselutedwithsolutionAandsolutionB40×30×30ml,respectively.TheelutingsolutionwascollectedandevaporatedtoneartodrynessinK-DConcentratorinwaterbathat45℃.Theresiduewasdissolvedandadjustedtoanappropriatevolumewithisooctane,andtheresultedsolutionwasreadyforthechromatographicdetermination.2.2.4column相关参数TheresultedsolutionwasdeterminedbyShimadzuGC-17AGasChromatographwithnitrogen-phosphorusdetector(NPD).Theconditionsforthegaschromatographwereasfollows:Column:DB-17capillarycolumn,15mlength,0.53mmi.d.,1μmfilmthickness(J&WScientificLtd.USA).Carriergas:nitrogen(99.999%),10ml/min.Detectorgases:air,145ml/min;hydrogen(99.99%),4.5ml/min;auxiliarygas,20ml/min.Temperature:oven,190℃;injector,250℃;detector,280℃.Attenuation:0Injectionvolume:1μlRetentiontime:6.084min.3通过国际习惯法检测coefficien,方法标准,即so岩与n-hex-methen费用,soinSamplesforrecoveryexperimentswereconducted,andtheresultsshowedthattheaveragerecoverypercentagebythemethodelutedwithsolutionA(n-hexaneanddiethylether)was97.96%withstandarddeviation2.94andcoefficientofvariation3.00%;thatwithsolutionB(n-hexaneanddichloromethane)was95.39%withstandarddeviation4.10andcoefficientofvariation4.30%(Table1).ThedetermineddatawereanalyzedbytheSRSsystem,andthedifferenceofaveragerecoverypercentagebetweensolutionAandsolutionBwasnotsignificant.Itshowedthatdichloromethanehadnodifferencefromdiethyletherinelutingmetalaxyl-M,soitcouldbeagoodsubstituteofdiethyletherindeterminingthemetalaxyl-M.Moreover,n-hexaneanddichloromethanemixedsolutioncouldbemoreeffectiveinelutingtheimpurityfromthelitchifleshthann-hexaneanddiethylethermixedsolution.Theminimalresolution(R)ofmetalaxyl-ManditsimpuritypeakfortifiedinlitchifleshwhichelutedwithsolutionAwas2.0,andthatwithsolutionBwas1.668,whichsuggestedthatthemetalaxyl-Mcouldcompletelyseparatedfromtheimpurityinthelitchiflesh(R>1.5).Smalllettermeansthesignificantdifferenceatlevel0.05.4u3000dichilusing、sochilusing、sochilusing、eth-案例性别不清非织造材料的保护Atthesamechromatographyconditions,usingtwoelutingsolutionA(n-hexaneanddiethylether)andsolutionB(n-hexaneanddichloromethane)producedthesameresultinelutingandpurifyingmetalaxyl-Minlitchifleshthroughacidicaluminach