镇痛药的镇痛作用

镇痛药的镇痛作用班级姓名实验要求：观察镇痛药的镇痛作用并联系临床应用。材料：恒温浴槽、0.4%哌替啶注射液。复方氨基比林注射液、生理盐水，小白鼠、1ml注射器、天平。实验操作：调节恒温浴槽的温度为55℃±0.5℃本组内分工，一人看表发令，一人拿鼠及观察，一人保持水温及记录结果。当看表人发令时，立即将一鼠放入恒温浴槽的热板上。以小鼠舔后脚趾为痛觉指标，计算从放入至有痛觉为止所需时间为痛阈值。用此法反应在40秒内小白鼠3只。依次测定每只小白鼠正常痛阈值，二次的平均值为给药前的痛阈值。按下述药物依次给药，每鼠每10分钟测定一次痛阈值，共60分钟。小白鼠药物用量用药方式甲鼠0.4%哌替啶溶液0.1ml/10g腹腔注射乙鼠复方氨基比林0.1ml/10g腹腔注射丙鼠生理盐水0.1mi/10g腹腔注射将结果记录于统计表上，并以全班各组痛阈值为计算依据。按下述公式计算痛阈值提高的百分率。 痛阈值提高百分率=（用药后痛阈值-用药前平均痛阈值）/用药前平均痛阈值*100%【说明】 如60秒内无反应，应把鼠取出，并按60为计算依据。【结果】实验数据：小组哌替啶复方氨基比林生理盐水t-检验:双样本异方差假设117.4614.009.50212.5018.0023.01变量1变量2313.1010.0012.36平均13.96511.85318182412.406.7019.50方差18.18491511.46530136516.508.009.50观测值1111612.5012.007.00假设平均差079.5016.007.91df1985.6610.8512.00tStat1.28629012915.0013.526.00P(T<=t)单尾0.1068979441021.0012.0019.50t单尾临界1.7291327921118.009.3220.18P(T<=t)双尾0.213795888t双尾临界2.0930240510min后小组哌替啶复方氨基比林生理盐水t-检验:双样本异方差假设12418.62602152012变量1变量23191764平均32.73727273222915.246.27方差355.4477218196.9088218524196观测值11116602015.24假设平均差07256012df18810.826.617tStat1.4965066439391411P(T<=t)单尾0.07592525710603312t单尾临界1.73406359211602010P(T<=t)双尾0.151850515t双尾临界2.10092203720min后小组哌替啶复方氨基比林生理盐水t-检验:双样本异方差假设134.822014.72260328变量1变量23232213.7平均37.1918181822.7436363643320.0520方差336.6311764190.8344455560608观测值111166015.029.25假设平均差072496df19823.292212.83tStat2.08647152191110.4938P(T<=t)单尾0.025325917105618.6210t单尾临界1.72913279211242160P(T<=t)双尾0.050651834t双尾临界2.0930240530min后小组哌替啶复方氨基比林生理盐水t-检验:双样本异方差假设12113.4110.442361860变量1变量231614.614平均32.1290909123.00727273421.791319方差162.5283691218.23544185281860观测值1111654.2514.6254.25假设平均差07464010df2082129.4550.72tStat1.550421998938.91725P(T<=t)单尾0.0683602611046607t单尾临界1.7247182181124.481560P(T<=t)双尾0.136720521t双尾临界2.08596344140min后小组哌替啶复方氨基比林生理盐水t-检验:双样本异方差假设1161360219327变量1变量23181912平均30.4081818219.0163636446016.4513.5方差253.840776485.507405455303710观测值1111615.7813.567.82假设平均差07332427df168601019tStat2.051004551926.610.222.5P(T<=t)单尾0.02850714510332427t单尾临界1.7458836691123.119.9713.7P(T<=t)双尾0.05701429t双尾临界2.11990528550min后小组哌替啶复方氨基比林生理盐水t-检验:双样本异方差假设115.1710.3820.282252814变量1变量2311.617.933.4平均28.4590909124.431818184601120方差302.8368891238.25441645252814观测值1111637.2813.4710.69假设平均差0717206df208606021.28tStat0.5742117919121510P(T<=t)单尾0.28611339510304510t单尾临界1.72471821811202060P(T<=t)双尾0.572226791t双尾临界2.085963441数据分析：哌替啶痛阈值提高百分率复方氨基比林痛阈值提高百分率生理盐水痛阈值提高百分率0分钟00010分钟131.74